Experiment Overview
| Repository ID: | FR-FCM-ZY6C | Experiment name: | Human melanoma mass cytometry analysis | MIFlowCyt score: | 49.25% |
| Primary researcher: | Spencer Wei | PI/manager: | Spencer Wei | Uploaded by: | Spencer Wei |
| Experiment dates: | 2017-05-02 - | Dataset uploaded: | May 2017 | Last updated: | Aug 2017 |
| Keywords: | [T cell] [mass cytometry] [CyTOF] [melanoma] [PD-1] [TIL] [CTLA-4] [checkpoint blockade] | Manuscripts: | |||
| Organizations: |
The University of Texas MD Anderson Cancer Center, Immunology, Houston, Texas (USA)
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| Purpose: | To comprehensively profile human melanoma tumor infiltrating T cell populations and identify T cell subsets responsive to checkpoint blockade using mass cytometry. | ||||
| Conclusion: | None | ||||
| Comments: | Analysis of on-therapy frozen TIL digests. Samples from patient 120 and 170 were analyzed in multiple staining reactions (individually barcoded). All other samples were analyzed as a single staining reactions. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Samples thawed, barcoded, and analyzed together. | ||||
Experiment variables
| Conditions | |
|---|---|
| · Normal donor PBMC | export_ND1_CD19- CD3+.fcs · export_ND2_CD19- CD3+.fcs · export_ND3_CD19- CD3+.fcs · c01_HuTILs_all.fcs · c02_HuTILs_all.fcs · c03_HuTILs_all.fcs |
| · Ipilimumab | export_120A5 a_CD19- CD3+.fcs · export_120A5 b_CD19- CD3+.fcs · export_120A5 c_CD19- CD3+.fcs · c04_HuTILs_all.fcs · c05_HuTILs_all.fcs · c06_HuTILs_all.fcs |
| · anti-PD-1 | export_224C_CD19- CD3+.fcs · export_251-3L_CD19- CD3+.fcs · export_267_CD19- CD3+.fcs · c07_HuTILs_all.fcs · c08_HuTILs_all.fcs · c09_HuTILs_all.fcs |
| · Ipilimumab + Nivolumab | export_170 a_CD19- CD3+.fcs · export_170 b_CD19- CD3+.fcs · export_170 c_CD19- CD3+.fcs · export_193_CD19- CD3+.fcs · export_227_CD19- CD3+.fcs · c10_HuTILs_all.fcs · c11_HuTILs_all.fcs · c12_HuTILs_all.fcs · c13_HuTILs_all.fcs · c14_HuTILs_all.fcs |
| Individuals | |
|---|---|
| · ND1 | export_ND1_CD19- CD3+.fcs · c01_HuTILs_all.fcs |
| · ND2 | export_ND2_CD19- CD3+.fcs · c02_HuTILs_all.fcs |
| · ND3 | export_ND3_CD19- CD3+.fcs · c03_HuTILs_all.fcs |
| · 120 | export_120A5 a_CD19- CD3+.fcs · export_120A5 b_CD19- CD3+.fcs · export_120A5 c_CD19- CD3+.fcs · c04_HuTILs_all.fcs · c05_HuTILs_all.fcs · c06_HuTILs_all.fcs |
| · 267 | export_267_CD19- CD3+.fcs · c07_HuTILs_all.fcs |
| · 224 | export_224C_CD19- CD3+.fcs · c08_HuTILs_all.fcs |
| · 251 | export_251-3L_CD19- CD3+.fcs · c09_HuTILs_all.fcs |
| · 227 | export_227_CD19- CD3+.fcs · c10_HuTILs_all.fcs |
| · 170 | export_170 a_CD19- CD3+.fcs · export_170 b_CD19- CD3+.fcs · export_170 c_CD19- CD3+.fcs · c11_HuTILs_all.fcs · c12_HuTILs_all.fcs · c13_HuTILs_all.fcs |
| · 193 | export_193_CD19- CD3+.fcs · c14_HuTILs_all.fcs |
| Sample Type | |
|---|---|
| · Bead normalized and debarcoded un-gated data | c01_HuTILs_all.fcs · c02_HuTILs_all.fcs · c03_HuTILs_all.fcs · c04_HuTILs_all.fcs · c05_HuTILs_all.fcs · c06_HuTILs_all.fcs · c07_HuTILs_all.fcs · c08_HuTILs_all.fcs · c09_HuTILs_all.fcs · c10_HuTILs_all.fcs · c11_HuTILs_all.fcs · c12_HuTILs_all.fcs · c13_HuTILs_all.fcs · c14_HuTILs_all.fcs |
| · T cell gated data with PhenoGraph and t-SNE parameters | export_120A5 a_CD19- CD3+.fcs · export_120A5 b_CD19- CD3+.fcs · export_120A5 c_CD19- CD3+.fcs · export_170 a_CD19- CD3+.fcs · export_170 b_CD19- CD3+.fcs · export_170 c_CD19- CD3+.fcs · export_193_CD19- CD3+.fcs · export_224C_CD19- CD3+.fcs · export_227_CD19- CD3+.fcs · export_251-3L_CD19- CD3+.fcs · export_267_CD19- CD3+.fcs · export_ND1_CD19- CD3+.fcs · export_ND2_CD19- CD3+.fcs · export_ND3_CD19- CD3+.fcs |
