Experiment Overview
| Repository ID: | FR-FCM-ZY9M | Experiment name: | CyTOF analysis of paired blood, tumor and non-involved lung T cells from NSCLC patients (gated CD3+ cells) | MIFlowCyt score: | 40.86% |
| Primary researcher: | Adeeb Rahman | PI/manager: | Adeeb Rahman | Uploaded by: | Adeeb Rahman |
| Experiment dates: | 2015-03-04 - 2016-03-11 | Dataset uploaded: | Aug 2017 | Last updated: | Aug 2017 |
| Keywords: | [mass cytometry] [CyTOF] [NSCLC] [adenocarcinoma] [human; IL-17A; Streptococcus pneumoniae; CD4 T cell; macrophage; Lung] | Manuscripts: | [28475900] | ||
| Organizations: |
Icahn School of Medicine at Mt. Sinai, New York, NY (10029)
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| Purpose: | To perform a detailed characterization of the T cell populations from paired blood, tumor and non-involved lung tissue from NSCLC patients | ||||
| Conclusion: | None | ||||
| Comments: | CD45-barcoded populations were deconvolved by Boolean gating. FCS files represent manually gated DNA+CD45+CD3+ from each tissue | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Single cell suspensions from paired blood, tumor and non-involved lung were barcoded with CD45 antibodies conjugated to distinct isotopes and pooled prior to staining and acquisition to minimize experimental variation between paired samples | ||||
Experiment variables
| Individuals | |
|---|---|
| · 236 | 236_Tcell_blood_Tcells-cd3.fcs · 236_Tcell_lung_Tcells-cd3.fcs · 236_Tcell_tumor_Tcells-cd3.fcs |
| · 237 | 237_Tcell_blood_Tcells-cd3.fcs · 237_Tcell_lung_Tcells-cd3.fcs · 237_Tcell_tumor_Tcells-cd3.fcs |
| · 245 | 245_Tcell_blood_Tcells-cd3.fcs · 245_Tcell_lung_Tcells-cd3.fcs · 245_Tcell_tumor_Tcells-cd3.fcs |
| · 246 | 246_Tcell_blood_Tcells-cd3.fcs · 246_Tcell_lung_Tcells-cd3.fcs · 246_Tcell_tumor_Tcells-cd3.fcs |
| · 249 | 249_Tcell_blood_Tcells-cd3.fcs · 249_Tcell_lung_Tcells-cd3.fcs · 249_Tcell_tumor_Tcells-cd3.fcs |
| · 250 | 250_Tcell_blood_Tcells-cd3.fcs · 250_Tcell_lung_Tcells-cd3.fcs · 250_Tcell_tumor_Tcells-cd3.fcs |
| · 251 | 251_Tcell_blood_Tcells-cd3.fcs · 251_Tcell_lung_Tcells-cd3.fcs · 251_Tcell_tumor_Tcells-cd3.fcs |
| · 253 | 253_Tcell_blood_Tcells-cd3.fcs · 253_Tcell_lung_Tcells-cd3.fcs · 253_Tcell_tumor_Tcells-cd3.fcs |
| · 255 | 255_Tcell_blood_Tcells-cd3.fcs · 255_Tcell_lung_Tcells-cd3.fcs · 255_Tcell_tumor_Tcells-cd3.fcs |
| · 256 | 256_Tcell_blood_Tcells-cd3.fcs · 256_Tcell_lung_Tcells-cd3.fcs · 256_Tcell_tumor_Tcells-cd3.fcs |
| · 257 | 257_Tcell_blood_Tcells-cd3.fcs · 257_Tcell_lung_Tcells-cd3.fcs · 257_Tcell_tumor_Tcells-cd3.fcs |
| · 258 | 258_Tcell_blood_Tcells-cd3.fcs · 258_Tcell_lung_Tcells-cd3.fcs · 258_Tcell_tumor_Tcells-cd3.fcs |
| · 259 | 259_Tcell_blood_Tcells-cd3.fcs · 259_Tcell_lung_Tcells-cd3.fcs · 259_Tcell_tumor_Tcells-cd3.fcs |
| · 260 | 260_Tcell_blood_Tcells-cd3.fcs · 260_Tcell_lung_Tcells-cd3.fcs · 260_Tcell_tumor_Tcells-cd3.fcs |
| · 261 | 261_Tcell_blood_Tcells-cd3.fcs · 261_Tcell_lung_Tcells-cd3.fcs · 261_Tcell_tumor_Tcells-cd3.fcs |
| · 262 | 262_Tcell_blood_Tcells-cd3.fcs · 262_Tcell_lung_Tcells-cd3.fcs · 262_Tcell_tumor_Tcells-cd3.fcs |
| · 263 | 263_Tcell_blood_Tcells-cd3.fcs · 263_Tcell_lung_Tcells-cd3.fcs · 263_Tcell_tumor_Tcells-cd3.fcs |
| · 266 | 266_Tcell_blood_Tcells-cd3.fcs · 266_Tcell_lung_Tcells-cd3.fcs · 266_Tcell_tumor_Tcells-cd3.fcs |
| · 267 | 267_Tcell_blood_Tcells-cd3.fcs · 267_Tcell_lung_Tcells-cd3.fcs · 267_Tcell_tumor_Tcells-cd3.fcs |
| · 291 | 291_Tcell_blood_Tcells-cd3.fcs · 291_Tcell_lung_Tcells-cd3.fcs · 291_Tcell_tumor_Tcells-cd3.fcs |
| · 296 | 296_Tcell_blood_Tcells-cd3.fcs · 296_Tcell_lung_Tcells-cd3.fcs · 296_Tcell_tumor_Tcells-cd3.fcs |
| · 308 | 308_Tcell_blood_cd3.fcs · 308_Tcell_lung_cd3.fcs · 308_Tcell_tumor_cd3.fcs |
| Sample Type | |
|---|---|
| · blood | 236_Tcell_blood_Tcells-cd3.fcs · 237_Tcell_blood_Tcells-cd3.fcs · 245_Tcell_blood_Tcells-cd3.fcs · 246_Tcell_blood_Tcells-cd3.fcs · 249_Tcell_blood_Tcells-cd3.fcs · 250_Tcell_blood_Tcells-cd3.fcs · 251_Tcell_blood_Tcells-cd3.fcs · 253_Tcell_blood_Tcells-cd3.fcs · 255_Tcell_blood_Tcells-cd3.fcs · 256_Tcell_blood_Tcells-cd3.fcs · 257_Tcell_blood_Tcells-cd3.fcs · 258_Tcell_blood_Tcells-cd3.fcs · 259_Tcell_blood_Tcells-cd3.fcs · 260_Tcell_blood_Tcells-cd3.fcs · 261_Tcell_blood_Tcells-cd3.fcs · 262_Tcell_blood_Tcells-cd3.fcs · 263_Tcell_blood_Tcells-cd3.fcs · 266_Tcell_blood_Tcells-cd3.fcs · 267_Tcell_blood_Tcells-cd3.fcs · 291_Tcell_blood_Tcells-cd3.fcs · 296_Tcell_blood_Tcells-cd3.fcs · 308_Tcell_blood_cd3.fcs |
| · lung | 236_Tcell_lung_Tcells-cd3.fcs · 237_Tcell_lung_Tcells-cd3.fcs · 245_Tcell_lung_Tcells-cd3.fcs · 246_Tcell_lung_Tcells-cd3.fcs · 249_Tcell_lung_Tcells-cd3.fcs · 250_Tcell_lung_Tcells-cd3.fcs · 251_Tcell_lung_Tcells-cd3.fcs · 253_Tcell_lung_Tcells-cd3.fcs · 255_Tcell_lung_Tcells-cd3.fcs · 256_Tcell_lung_Tcells-cd3.fcs · 257_Tcell_lung_Tcells-cd3.fcs · 258_Tcell_lung_Tcells-cd3.fcs · 259_Tcell_lung_Tcells-cd3.fcs · 260_Tcell_lung_Tcells-cd3.fcs · 261_Tcell_lung_Tcells-cd3.fcs · 262_Tcell_lung_Tcells-cd3.fcs · 263_Tcell_lung_Tcells-cd3.fcs · 266_Tcell_lung_Tcells-cd3.fcs · 267_Tcell_lung_Tcells-cd3.fcs · 291_Tcell_lung_Tcells-cd3.fcs · 296_Tcell_lung_Tcells-cd3.fcs · 308_Tcell_lung_cd3.fcs |
| · tumor | 236_Tcell_tumor_Tcells-cd3.fcs · 237_Tcell_tumor_Tcells-cd3.fcs · 245_Tcell_tumor_Tcells-cd3.fcs · 246_Tcell_tumor_Tcells-cd3.fcs · 249_Tcell_tumor_Tcells-cd3.fcs · 250_Tcell_tumor_Tcells-cd3.fcs · 251_Tcell_tumor_Tcells-cd3.fcs · 253_Tcell_tumor_Tcells-cd3.fcs · 255_Tcell_tumor_Tcells-cd3.fcs · 256_Tcell_tumor_Tcells-cd3.fcs · 257_Tcell_tumor_Tcells-cd3.fcs · 258_Tcell_tumor_Tcells-cd3.fcs · 259_Tcell_tumor_Tcells-cd3.fcs · 260_Tcell_tumor_Tcells-cd3.fcs · 261_Tcell_tumor_Tcells-cd3.fcs · 262_Tcell_tumor_Tcells-cd3.fcs · 263_Tcell_tumor_Tcells-cd3.fcs · 266_Tcell_tumor_Tcells-cd3.fcs · 267_Tcell_tumor_Tcells-cd3.fcs · 291_Tcell_tumor_Tcells-cd3.fcs · 296_Tcell_tumor_Tcells-cd3.fcs · 308_Tcell_tumor_cd3.fcs |
