Experiment Overview
| Repository ID: | FR-FCM-ZZ3L | Experiment name: | Nile Red fluorescent nanoparticles | MIFlowCyt score: | 73.33% |
| Primary researcher: | John Nolan | PI/manager: | John Nolan | Uploaded by: | John Nolan |
| Experiment dates: | 2011-02-23 - 2011-02-23 | Dataset uploaded: | Oct 2012 | Last updated: | Jan 2013 |
| Keywords: | [nanoparticle] [trigger] [fluorescence] [light scatter] | Manuscripts: | [23335161] [PMC3578978] |
|
|
| Organizations: |
La Jolla Bioengineering Institute, San Diego, California (USA)
|
||||
| Purpose: | To thest the ability to detect and measure fluorescent nanoparticles using light scatter or fluorescecne-based triggering. | ||||
| Conclusion: | Choice of trigger channel can affect results | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | 8 pk Rainbow beads (Spherotech) cross calibrated against PE Quantibrite beads (BD Biosciences) were measured and used to calculate PE-MESF values. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · 0.53 um NR beads | 022311ss.037 · 022311ss.038 · 022311ss.039 · 022311ss.040 · 022311ss.041 · 022311ss.042 · 022311ss.043 · 022311ss.044 · 022311ss.045 · 022311ss.046 · 022311ss.047 · 022311ss.048 · 022311ss.049 · 022311ss.050 · 022311ss.051 · 022311ss.052 · 022311ss.053 · 022311ss.054 |
| · 0.11 um NR beads | 022311ss.055 · 022311ss.056 · 022311ss.057 · 022311ss.058 · 022311ss.059 · 022311ss.060 · 022311ss.061 · 022311ss.062 · 022311ss.063 · 022311ss.064 · 022311ss.065 · 022311ss.066 · 022311ss.067 · 022311ss.068 · 022311ss.069 · 022311ss.070 · 022311ss.071 · 022311ss.072 |
| Conditions | |
|---|---|
| · 1:2 | 022311ss.037 · 022311ss.046 · 022311ss.055 · 022311ss.064 |
| · 1:4 | 022311ss.038 · 022311ss.047 · 022311ss.056 · 022311ss.065 |
| · 1:8 | 022311ss.039 · 022311ss.048 · 022311ss.057 · 022311ss.066 |
| · 1:16 | 022311ss.040 · 022311ss.049 · 022311ss.058 · 022311ss.067 |
| · 1:32 | 022311ss.041 · 022311ss.050 · 022311ss.059 · 022311ss.068 |
| · 1: 64 | 022311ss.042 · 022311ss.051 · 022311ss.060 · 022311ss.069 |
| · 1:128 | 022311ss.043 · 022311ss.052 · 022311ss.061 · 022311ss.070 |
| · 1:256 | 022311ss.044 · 022311ss.053 · 022311ss.062 · 022311ss.071 |
| · buffer only | 022311ss.045 · 022311ss.054 · 022311ss.063 · 022311ss.072 |
| Individuals | |
|---|---|
| · FSC/SSC Trigger | 022311ss.037 · 022311ss.038 · 022311ss.039 · 022311ss.040 · 022311ss.041 · 022311ss.042 · 022311ss.043 · 022311ss.044 · 022311ss.045 · 022311ss.055 · 022311ss.056 · 022311ss.057 · 022311ss.058 · 022311ss.059 · 022311ss.060 · 022311ss.061 · 022311ss.062 · 022311ss.063 |
| · FL Trigger | 022311ss.046 · 022311ss.047 · 022311ss.048 · 022311ss.049 · 022311ss.050 · 022311ss.051 · 022311ss.052 · 022311ss.053 · 022311ss.054 · 022311ss.064 · 022311ss.065 · 022311ss.066 · 022311ss.067 · 022311ss.068 · 022311ss.069 · 022311ss.070 · 022311ss.071 · 022311ss.072 |
