Experiment Overview
| Repository ID: | FR-FCM-ZZ3M | Experiment name: | EMBO Timecourse of IL-4 and IL-7 in PBMC B and T cells | MIFlowCyt score: | 47.00% |
| Primary researcher: | Josef Spidlen | PI/manager: | Josef Spidlen | Uploaded by: | Josef Spidlen |
| Experiment dates: | 2010-03-01 - 2010-05-31 | Dataset uploaded: | Oct 2012 | Last updated: | Apr 2016 |
| Keywords: | [B cells] [T cell] [PBMC] [EMBO] [CD5] [CD20] | Manuscripts: | |||
| Organizations: |
BC Cancer Agency, Terry Fox Lab, Vancouver, BC (Canada)
Cytobank Inc., Mountain View, California (USA) |
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| Purpose: | Stimulate human peripheral blood mononuclear cells (PBMCs) with two cytokines, IL-4 and IL-7, and compare signaling over time in B cells and T cells. | ||||
| Conclusion: | B cells were identified as CD20+ CD5- (B2 B cells) and T cells were identified as CD5+ CD20-. Other PBMCs are also present within the tube (CD5- CD20-). | ||||
| Comments: | Cytobank experiment number 336 | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Conditions | |
|---|---|
| · IL-4 | EMBO Group1 Signaling timecourse_1-01 IL-4 Unstimulated 0 min.fcs · EMBO Group1 Signaling timecourse_1-02 IL-4 1 min.fcs · EMBO Group1 Signaling timecourse_1-03 IL-4 2 min.fcs · EMBO Group1 Signaling timecourse_1-04 IL-4 4 min.fcs · EMBO Group1 Signaling timecourse_1-05 IL-4 8 min.fcs · EMBO Group1 Signaling timecourse_1-06 IL-4 12 min.fcs · EMBO Group1 Signaling timecourse_1-07 IL-4 16 min.fcs |
| · IL-7 | EMBO Group1 Signaling timecourse_1-08 IL-7 Unstimulated 0 min.fcs · EMBO Group1 Signaling timecourse_1-09 IL-7 1 min.fcs · EMBO Group1 Signaling timecourse_1-10 IL-7 2 min.fcs · EMBO Group1 Signaling timecourse_1-11 IL-7 4 min.fcs · EMBO Group1 Signaling timecourse_1-12 IL-7 8 min.fcs · EMBO Group1 Signaling timecourse_1-13 IL-7 12 min.fcs · EMBO Group1 Signaling timecourse_1-14 IL-7 16 min.fcs |
| Timepoints | |
|---|---|
| · Unstim | EMBO Group1 Signaling timecourse_1-01 IL-4 Unstimulated 0 min.fcs · EMBO Group1 Signaling timecourse_1-08 IL-7 Unstimulated 0 min.fcs |
| · 1 min | EMBO Group1 Signaling timecourse_1-02 IL-4 1 min.fcs · EMBO Group1 Signaling timecourse_1-09 IL-7 1 min.fcs |
| · 2 min | EMBO Group1 Signaling timecourse_1-03 IL-4 2 min.fcs · EMBO Group1 Signaling timecourse_1-10 IL-7 2 min.fcs |
| · 4 min | EMBO Group1 Signaling timecourse_1-04 IL-4 4 min.fcs · EMBO Group1 Signaling timecourse_1-11 IL-7 4 min.fcs |
| · 8 min | EMBO Group1 Signaling timecourse_1-05 IL-4 8 min.fcs · EMBO Group1 Signaling timecourse_1-12 IL-7 8 min.fcs |
| · 12 min | EMBO Group1 Signaling timecourse_1-06 IL-4 12 min.fcs · EMBO Group1 Signaling timecourse_1-13 IL-7 12 min.fcs |
| · 16 min | EMBO Group1 Signaling timecourse_1-07 IL-4 16 min.fcs · EMBO Group1 Signaling timecourse_1-14 IL-7 16 min.fcs |
