Experiment Overview
| Repository ID: | FR-FCM-ZZ64 | Experiment name: | Non-specific intracellular staining of plasma cells by PE | MIFlowCyt score: | 25.50% |
| Primary researcher: | MS Kim | PI/manager: | MS Kim | Uploaded by: | MS Kim |
| Experiment dates: | 2011-01-01 - 2012-09-30 | Dataset uploaded: | Feb 2013 | Last updated: | Mar 2013 |
| Keywords: | [phycoerythrin; non-specific binding; plasma cells; intracellular staining] | Manuscripts: | [23463627] |
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| Organizations: |
Korea University, Seoul, (Republic of Korea)
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| Purpose: | To identify the cell type which was non-specifically stained by PE-conjugated antibody and show that PE-conjugated antibodies are not proper for intracellular staining of immune cells. | ||||
| Conclusion: | PE-conjugated antibodies are inappropriate for intracellular staining of murine plasma cells and activated B cells. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Sample Type | |
|---|---|
| · mouse small intestinal lamina propria cells | F1A CD4F FOXPE 138A.fcs.001 · F1A CD4F ISO-PE 138A.fcs · F1B INTRA-IgAFI FOXPE LY6CAPC.fcs · F1B IgAFI FOXPE LY6CAPC.fcs · F1D B220.fcs · F1D CD138.fcs · F1D Ly6c.fcs · F1D cd11b.fcs · F1D cd11c.fcs · F1D cd4.fcs · F1D cd8.fcs · F1D f480.fcs · F2A BALBC.fcs · F2A b6.fcs · F2A c3h.fcs · F2C LP hCD123F CD138A.fcs · F2C LP hCD123PE CD138A.fcs · F5C 0WK IgAF PE 138A.fcs · F5C 1WK IgAF PE 138A.fcs · F5C 2WK IgAF PE 138A.fcs · F5C 3WK IgAF PE 138A.fcs · FS1A CD4FI FOXPE LY6CAPC.fcs · FS1B AXV-FI CD25APC FOXPE.fcs.001 · FS2A PE.fcs · FS2B IgAF FOXP3PE 138A.fcs · FS2B IgAF ISOPE 138A.fcs · FS3 CD4F FOXP 138A SURFACE.fcs · FS4B IgAF PEintra 138A.fcs · FS4B IgAF PEsurface 138A.fcs · FS5A BLOCK10MIN CONPE CD138A.fcs · FS5A BLOCK30MIN CONPE CD138A.fcs · FS5A BLOCK60MIN CONPE CD138A.fcs · FS5A STANDARD CONPE CD138A.fcs.001 · FS5B 0-1 TX100 CD4F ISOPE 138A.fcs · FS5B 0-3 TX100 CD4F ISOPE 138A.fcs · FS5B 0-5 TX100 CD4F ISOPE 138A.fcs · FS5B STD WASH CD4F ISOPE 138A.fcs.001 · FS6 IFNPE 1-100 CD4F CD138.fcs · FS6 IFNPE 1-1600 CD4F CD138.fcs · FS6 IFNPE 1-200 CD4F CD138.fcs · FS6 IFNPE 1-3200 CD4F CD138.fcs · FS6 IFNPE 1-400 CD4F CD138.fcs · FS6 IFNPE 1-6400 CD4F CD138.fcs · FS6 IFNPE 1-800 CD4F CD138.fcs |
| · mouse splenocytes | F3A sp ISO-PE CD138A.fcs · F4A 0d CD19APC PE.fcs · F4A 1d CD19APC PE.fcs · F4A 2d CD19APC PE.fcs · F4A 3d CD19APC PE.fcs · FS7 - SP IgAF PE 138A.fcs · FS7 SP IgAF PE 138A.fcs |
| · mouse bone marrow cells | F3A BM ISO-PE CD138A.fcs · FS7 - BM IgAF PE 138A.fcs · FS7 BM IgAF PE 138A.fcs |
| · mouse mesenteric lymph node cells | F3A MLN ISO-PE CD138A.fcs · FS7 - MLN IgAF PE 138A.fcs · FS7 MLN IgAF PE 138A.fcs |
| · mouse peyer's patch cells | FS7 - PP IgAF PE 138A.fcs · FS7 PP IgAF PE 138A.fcs |
| · mouse peripheral lymph node cells | FS7 - LN IgAF PE 138A.fcs · FS7 LN IgAF PE 138A.fcs |
