Experiment Overview
| Repository ID: | FR-FCM-ZZ85 | Experiment name: | Detection of CFTR protein in leukocytes by flow cytometry | MIFlowCyt score: | 90.75% |
| Primary researcher: | Jan Johansson | PI/manager: | Jan Johansson | Uploaded by: | Jan Johansson |
| Experiment dates: | 2013-06-25 - 2013-06-28 | Dataset uploaded: | Oct 2013 | Last updated: | Feb 2014 |
| Keywords: | [cystic fibrosis CFTR monocytes blocking peptide CD14] | Manuscripts: | [24532172] |
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| Organizations: |
University of Verona, Italy, Dept of Pathology, Verona, (Italy)
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| Purpose: | Test CFTR expression in human monocytes and specificity of anti-CFTR antibody | ||||
| Conclusion: | A specific signal of CFTR expression was observed in monocytes | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Instrument calibration with beads was performed | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Anti-CFTR antibody preincubated with blocking peptide | CF+blocking peptide.fcs · nonCF+blocking peptide.fcs |
| · Anti-CFTR antibody not preincubated with blocking peptide | CF.fcs · nonCF.fcs |
| Individuals | |
|---|---|
| · Individual carrying nonsense CFTR mutation on both CFTR alleles | CF+blocking peptide.fcs · CF.fcs |
| · Individual without cystic fibrosis phenotype | nonCF+blocking peptide.fcs · nonCF.fcs |
