Experiment Overview
| Repository ID: | FR-FCM-ZZAA | Experiment name: | Effect of CA on Human IL-22 in Cord Blood | MIFlowCyt score: | 93.62% |
| Primary researcher: | Margaret Lowe | PI/manager: | Margaret Lowe | Uploaded by: | Margaret Lowe |
| Experiment dates: | 2013-08-01 - 2013-10-04 | Dataset uploaded: | Mar 2014 | Last updated: | Apr 2014 |
| Keywords: | [human] [IL-22] [IL-17] [cord blood] [polarizing conditions] | Manuscripts: | [24498387] [PMC3912126] |
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| Organizations: |
University of California, San Francisco, San Francisco, CA (United States)
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| Purpose: | To determine the effect of CA on IL-22 production by naive CD4+ T cells sort-purified from human cord blood and stimulated under polarizing conditions | ||||
| Conclusion: | CA is capable of stimulating IL-22 production from naive CD4+ T cells from human cord blood; the effect is dose-dependent. | ||||
| Comments: | See Figures 8C and 8D of Lowe et al PLoS One 2014. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Doses | |
|---|---|
| · 0.5 µM | cb_CA 0-5.fcs · cb c_ca 0-5.fcs · cb1_CA 0-5.fcs · cb12_CA 0-5.fcs · cb53_CA 0-5.fcs · cb56_CA 0-5.fcs |
| · 1 µM | cb_CA 1.fcs · cb c_ca 1.fcs · cb1_CA 1.fcs · cb12_CA 1.fcs · cb53_CA 1.fcs · cb56_CA 1.fcs |
| · 5 µM | cb_CA 5.fcs · cb c_ca 5.fcs · cb1_CA 5.fcs · cb12_CA 5.fcs · cb53_CA 5.fcs · cb56_CA 5.fcs |
| · 10 µM | CB47_CA 10.fcs · CB47_KYA 10.fcs · CB47_LKYN 10.fcs · cb_CA 10.fcs · cb_KYA 10.fcs · cb_LKYN 10.fcs · cb8_CA 10.fcs · cb8_KYA 10.fcs · cb8_LKYN 10.fcs · cb_CA 10.fcs-(1) · cb_KYA 10.fcs-(1) · cb_LKYN 10.fcs-(1) · cb 8 sfgh_CA 10.fcs · cb 8 sfgh_KYA 10.fcs · cb 8 sfgh_LKYN 10.fcs · cb c_CA 10.fcs · cb9_CA 10.fcs · cb9_DMSO 10.fcs · cb9_KYA 10.fcs · cb9_LKYN 10.fcs · CB 52_CA 10.fcs · CB 52_KYA 10.fcs · CB 52_LKYN 10.fcs · CB 53_CA 10.fcs · CB 53_KYA 10.fcs · CB 53_LKYN 10.fcs · cb1_CA 10.fcs · cb1_kya 10.fcs · cb1_kyn 10.fcs · cb12_CA 10.fcs · cb53_CA 10.fcs · cb56_CA 10.fcs |
| · 25 µM | cb_KYA 25.fcs · cb_KYN 25.fcs · cb8_CA 25.fcs · cb8_KYA 25.fcs · cb8_KYN 25.fcs · cb_KYA 25.fcs-(1) · cb_KYN 25.fcs-(1) · CB 53_KYA 25.fcs · CB 53_LKYN 25.fcs · cb1_kya 25.fcs · cb1_kyn25.fcs |
| · Vehicle | CB47_DMSO.fcs · cb_DMSO 25.fcs · cb_DMSO.fcs · cb8_DMSO 25.fcs · cb8_DMSO.fcs · cb_DMSO 25.fcs-(1) · cb_DMSO.fcs-(1) · cb 8 sfgh_DMSO.fcs · cb c_dmso.fcs · CB 52_DMSO.fcs · CB 53_DMSO 10.fcs · CB 53_DMSO 25.fcs · cb1_DMSO.fcs · cb12_DMSO.fcs · cb53_DMSO.fcs · cb56_DMSO.fcs |
| · 0.25 µM | cb1_ca 0-25.fcs |
| Conditions | |
|---|---|
| · DMSO | CB47_DMSO.fcs · cb_DMSO 25.fcs · cb_DMSO.fcs · cb8_DMSO 25.fcs · cb8_DMSO.fcs · cb_DMSO 25.fcs-(1) · cb_DMSO.fcs-(1) · cb 8 sfgh_DMSO.fcs · cb c_dmso.fcs · cb9_DMSO 10.fcs · CB 52_DMSO.fcs · CB 53_DMSO 10.fcs · CB 53_DMSO 25.fcs · cb1_DMSO.fcs · cb12_DMSO.fcs · cb53_DMSO.fcs · cb56_DMSO.fcs |
| · CA | CB47_CA 10.fcs · cb_CA 10.fcs · cb8_CA 10.fcs · cb8_CA 25.fcs · cb_CA 0-5.fcs · cb_CA 1.fcs · cb_CA 10.fcs-(1) · cb_CA 5.fcs · cb 8 sfgh_CA 10.fcs · cb c_CA 10.fcs · cb c_ca 0-5.fcs · cb c_ca 1.fcs · cb c_ca 5.fcs · cb9_CA 10.fcs · CB 52_CA 10.fcs · CB 53_CA 10.fcs · cb1_CA 0-5.fcs · cb1_CA 1.fcs · cb1_CA 10.fcs · cb1_CA 5.fcs · cb1_ca 0-25.fcs · cb12_CA 0-5.fcs · cb12_CA 1.fcs · cb12_CA 10.fcs · cb12_CA 5.fcs · cb53_CA 0-5.fcs · cb53_CA 1.fcs · cb53_CA 10.fcs · cb53_CA 5.fcs · cb56_CA 0-5.fcs · cb56_CA 1.fcs · cb56_CA 10.fcs · cb56_CA 5.fcs |
| · KYA | CB47_KYA 10.fcs · cb_KYA 10.fcs · cb_KYA 25.fcs · cb8_KYA 10.fcs · cb8_KYA 25.fcs · cb_KYA 10.fcs-(1) · cb_KYA 25.fcs-(1) · cb 8 sfgh_KYA 10.fcs · cb9_KYA 10.fcs · CB 52_KYA 10.fcs · CB 53_KYA 10.fcs · CB 53_KYA 25.fcs · cb1_kya 10.fcs · cb1_kya 25.fcs |
| · L-KYN | CB47_LKYN 10.fcs · cb_KYN 25.fcs · cb_LKYN 10.fcs · cb8_KYN 25.fcs · cb8_LKYN 10.fcs · cb_KYN 25.fcs-(1) · cb_LKYN 10.fcs-(1) · cb 8 sfgh_LKYN 10.fcs · cb9_LKYN 10.fcs · CB 52_LKYN 10.fcs · CB 53_LKYN 10.fcs · CB 53_LKYN 25.fcs · cb1_kyn 10.fcs · cb1_kyn25.fcs |
