Experiment Overview
| Repository ID: | FR-FCM-ZZAF | Experiment name: | Comparison of CA and FICZ | MIFlowCyt score: | 75.20% |
| Primary researcher: | Margaret Lowe | PI/manager: | Margaret Lowe | Uploaded by: | Margaret Lowe |
| Experiment dates: | 2012-07-06 - 2012-08-10 | Dataset uploaded: | Mar 2014 | Last updated: | Aug 2014 |
| Keywords: | [mouse T cells] [IL-22] [IL-17] [CA] [FICZ] | Manuscripts: | [24498387] [PMC3912126] |
|
|
| Organizations: |
University of California, San Francisco, San Francisco, CA (United States)
|
||||
| Purpose: | To compare effects of CA versus FICZ on IL-17 and IL-22 production on mouse naive CD4 T cells differentiated under polarizing conditions in vitro | ||||
| Conclusion: | CA has equivalent effects to FICZ on IL-22 production. | ||||
| Comments: | See Figure 8E in Lowe et al PLoS One 2014 | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Doses | |
|---|---|
| · 10 µM | Day 5_CA 10.fcs · E1_CA 10.fcs · E2_CA 10.fcs · no IFNg stain_CA 10.fcs · no gamma stain_CA 10.fcs · no gamma_CA 10.fcs |
| · 25 µM | Day 5_CA 25.fcs · E1_CA 25.fcs · E2_CA 25.fcs · no IFNg stain_CA 25.fcs · no gamma stain_CA 25.fcs · no gamma_CA 25.fcs |
| · 250 nM | Day 5_FICZ 250 nM.fcs · E1_FICZ.fcs · E2_FICZ.fcs · no IFNg stain_FICZ.fcs · no gamma stain_FICZ 250.fcs · no gamma_FICZ 250 nM.fcs |
| · vehicle | Day 5_DMSO.fcs · E1_DMSO.fcs · E2_DMSO.fcs · no IFNg stain_DMSO.fcs · no gamma stain_DMSO.fcs · no gamma_DMSO.fcs |
| Conditions | |
|---|---|
| · DMSO | Day 5_DMSO.fcs · E1_DMSO.fcs · E2_DMSO.fcs · no IFNg stain_DMSO.fcs · no gamma stain_DMSO.fcs · no gamma_DMSO.fcs |
| · CA | Day 5_CA 10.fcs · Day 5_CA 25.fcs · E1_CA 10.fcs · E1_CA 25.fcs · E2_CA 10.fcs · E2_CA 25.fcs · no IFNg stain_CA 10.fcs · no IFNg stain_CA 25.fcs · no gamma stain_CA 10.fcs · no gamma stain_CA 25.fcs · no gamma_CA 10.fcs · no gamma_CA 25.fcs |
| · FICZ | Day 5_FICZ 250 nM.fcs · E1_FICZ.fcs · E2_FICZ.fcs · no IFNg stain_FICZ.fcs · no gamma stain_FICZ 250.fcs · no gamma_FICZ 250 nM.fcs |
