Experiment Overview
| Repository ID: | FR-FCM-ZZAG | Experiment name: | Effects of CA on Mouse Treg Differentiation | MIFlowCyt score: | 81.11% |
| Primary researcher: | Margaret Lowe | PI/manager: | Margaret Lowe | Uploaded by: | Margaret Lowe |
| Experiment dates: | 2013-02-08 - 2013-03-06 | Dataset uploaded: | Mar 2014 | Last updated: | Aug 2014 |
| Keywords: | [FoxP3] [mouse T cells] [CA] [Treg] [TGFb] | Manuscripts: | [24498387] [PMC3912126] |
|
|
| Organizations: |
University of California, San Francisco, San Francisco, CA (United States)
|
||||
| Purpose: | To determine the effects of CA on FOXP3 expression in mouse naive T cells stimulated with or without TGFbeta | ||||
| Conclusion: | CA does not increase mouse Treg differentiation. | ||||
| Comments: | See figure 6D of Lowe et al Plos One 2014 | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Doses | |
|---|---|
| · 10 µM | Treg_CA10 b0.fcs · Treg_CA10 b0.fcs-(1) · Treg_CA10 b10.fcs · Treg_CA10 b10.fcs-(1) · Treg_CA10 b5.fcs · Treg_CA10 b5.fcs-(1) · beta 0_CA 10.fcs · beta 0_CA 10.fcs-(1) · beta 10_CA 10.fcs · beta 10_CA 10.fcs-(1) · beta 5_CA 10.fcs · beta 5_CA 10.fcs-(1) |
| · 25 µM | Treg_CA 25 b0.fcs · Treg_CA 25 b0.fcs-(1) · Treg_CA 25 b10.fcs · Treg_CA 25 b10.fcs-(1) · Treg_CA 25 b5.fcs · Treg_CA 25 b5.fcs-(1) · beta 0_CA 25.fcs · beta 0_CA 25.fcs-(1) · beta 10_CA 25.fcs · beta 10_CA 25.fcs-(1) · beta 5_CA 25.fcs · beta 5_CA 25.fcs-(1) |
| · vehicle | Treg_D B0.fcs · Treg_D B0.fcs-(1) · Treg_D B10.fcs · Treg_D B10.fcs-(1) · Treg_D B5.fcs · Treg_D B5.fcs-(1) · beta 0_DMSO.fcs · beta 0_DMSO.fcs-(1) · beta 10_DMSO.fcs · beta 10_DMSO.fcs-(1) · beta 5_DMSO.fcs · beta 5_DMSO.fcs-(1) |
| Sample Type | |
|---|---|
| · CA | Treg_CA 25 b0.fcs · Treg_CA 25 b0.fcs-(1) · Treg_CA 25 b10.fcs · Treg_CA 25 b10.fcs-(1) · Treg_CA 25 b5.fcs · Treg_CA 25 b5.fcs-(1) · Treg_CA10 b0.fcs · Treg_CA10 b0.fcs-(1) · Treg_CA10 b10.fcs · Treg_CA10 b10.fcs-(1) · Treg_CA10 b5.fcs · Treg_CA10 b5.fcs-(1) · beta 0_CA 10.fcs · beta 0_CA 10.fcs-(1) · beta 0_CA 25.fcs · beta 0_CA 25.fcs-(1) · beta 10_CA 10.fcs · beta 10_CA 10.fcs-(1) · beta 10_CA 25.fcs · beta 10_CA 25.fcs-(1) · beta 5_CA 10.fcs · beta 5_CA 10.fcs-(1) · beta 5_CA 25.fcs · beta 5_CA 25.fcs-(1) |
| · DMSO | Treg_D B0.fcs · Treg_D B0.fcs-(1) · Treg_D B10.fcs · Treg_D B10.fcs-(1) · Treg_D B5.fcs · Treg_D B5.fcs-(1) · beta 0_DMSO.fcs · beta 0_DMSO.fcs-(1) · beta 10_DMSO.fcs · beta 10_DMSO.fcs-(1) · beta 5_DMSO.fcs · beta 5_DMSO.fcs-(1) |
| Conditions | |
|---|---|
| · No TGFβ | Treg_CA 25 b0.fcs · Treg_CA 25 b0.fcs-(1) · Treg_CA10 b0.fcs · Treg_CA10 b0.fcs-(1) · Treg_D B0.fcs · Treg_D B0.fcs-(1) · beta 0_CA 10.fcs · beta 0_CA 10.fcs-(1) · beta 0_CA 25.fcs · beta 0_CA 25.fcs-(1) · beta 0_DMSO.fcs · beta 0_DMSO.fcs-(1) |
| · 5 ng/mL TGFβ | Treg_CA 25 b5.fcs · Treg_CA 25 b5.fcs-(1) · Treg_CA10 b5.fcs · Treg_CA10 b5.fcs-(1) · Treg_D B5.fcs · Treg_D B5.fcs-(1) · beta 5_CA 10.fcs · beta 5_CA 10.fcs-(1) · beta 5_CA 25.fcs · beta 5_CA 25.fcs-(1) · beta 5_DMSO.fcs · beta 5_DMSO.fcs-(1) |
| · 10 ng/mL TGFβ | Treg_CA 25 b10.fcs · Treg_CA 25 b10.fcs-(1) · Treg_CA10 b10.fcs · Treg_CA10 b10.fcs-(1) · Treg_D B10.fcs · Treg_D B10.fcs-(1) · beta 10_CA 10.fcs · beta 10_CA 10.fcs-(1) · beta 10_CA 25.fcs · beta 10_CA 25.fcs-(1) · beta 10_DMSO.fcs · beta 10_DMSO.fcs-(1) |
