Experiment Overview

Repository ID: FR-FCM-ZZBN Experiment name: Selection on noise constrains variation in a yeast promoter MIFlowCyt score: 49.25%
Primary researcher: Brian Metzger PI/manager: Patricia Wittkopp Uploaded by: Brian Metzger
Experiment dates: 2013-01-01 - 2013-03-31 Dataset uploaded: Apr 2014 Last updated: Apr 2015
Keywords: [Ancestral state reconstruction] [Mutation] [Evolution] Manuscripts: [25778704] Naturelogo
Organizations: University of Michigan, Ann Arbor, MI (USA)
Purpose: Quantify expression of Saccharomyces cerevisiae TDH3 promoter variants.
Conclusion: Natural selection on gene expression noise has had a greater impact on TDH3 natural variation than selection on mean expression level.
Comments: All samples were revived from glycerol stocks on the same day to control for age related effects on expression. Samples were grown in YFP and run in SC-R liquid media.
Funding: March of Dimes (5-FY07-181) Alfred P. Sloan Research Foundation National Science Foundation (MCB-1021398), Genome Sciences training grant (T32 HG000040) National Institutes of Health Genetics training grant (T32 GM007544) National Institutes of Health NRSA postdoctoral fellowship (1 F32 GM083513-0) EMBO postdoctoral fellowship (EMBO ALTF 1114-2012)
Quality control: Each plate contains 20 genetically identical samples at fixed positions used to correct for batch effects.
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