Experiment Overview
| Repository ID: | FR-FCM-ZZBN | Experiment name: | Selection on noise constrains variation in a yeast promoter | MIFlowCyt score: | 49.25% |
| Primary researcher: | Brian Metzger | PI/manager: | Patricia Wittkopp | Uploaded by: | Brian Metzger |
| Experiment dates: | 2013-01-01 - 2013-03-31 | Dataset uploaded: | Apr 2014 | Last updated: | Apr 2015 |
| Keywords: | [Ancestral state reconstruction] [Mutation] [Evolution] | Manuscripts: | [25778704] |
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| Organizations: |
University of Michigan, Ann Arbor, MI (USA)
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| Purpose: | Quantify expression of Saccharomyces cerevisiae TDH3 promoter variants. | ||||
| Conclusion: | Natural selection on gene expression noise has had a greater impact on TDH3 natural variation than selection on mean expression level. | ||||
| Comments: | All samples were revived from glycerol stocks on the same day to control for age related effects on expression. Samples were grown in YFP and run in SC-R liquid media. | ||||
| Funding: | March of Dimes (5-FY07-181) Alfred P. Sloan Research Foundation National Science Foundation (MCB-1021398), Genome Sciences training grant (T32 HG000040) National Institutes of Health Genetics training grant (T32 GM007544) National Institutes of Health NRSA postdoctoral fellowship (1 F32 GM083513-0) EMBO postdoctoral fellowship (EMBO ALTF 1114-2012) | ||||
| Quality control: | Each plate contains 20 genetically identical samples at fixed positions used to correct for batch effects. | ||||
