Experiment Overview
| Repository ID: | FR-FCM-ZZC4 | Experiment name: | RNA Flow Cytometry: single RNA stained samples vs multiplexed | MIFlowCyt score: | 96.14% |
| Primary researcher: | Chip Lomas | PI/manager: | Emily Park | Uploaded by: | Chip Lomas |
| Experiment dates: | 2012-07-06 - 2012-07-06 | Dataset uploaded: | May 2014 | Last updated: | May 2014 |
| Keywords: | [Gene expression] [RNA Amplification] [RNA hybridization] [RNA Flow cytometry] | Manuscripts: | [23441230] [PMC3575505] |
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| Organizations: |
BD Biosciences, Research and Development, San Jose, CA (USA)
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| Purpose: | The purpose of this experiment was to determine the effects of staining with multiple RNA probes vs single probes on MFIs in RNA flow cytometry. | ||||
| Conclusion: | There was no difference in MFI observed when multiplexing probes, compared to single probes. | ||||
| Comments: | Detection of Low Abundance RNA Molecules in Individual Cells by Flow Cytometry Figure 4c | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Cytometer was setup using BD Cytometer Setup and Tracking (CST) in FACSDiva software. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · K562 cell line | K562_3 color-1.fcs · K562_3 color-2.fcs · K562_18s-1.fcs · K562_18s-2.fcs · K562_ABL-1.fcs · K562_ABL-2.fcs · K562_BCR-1.fcs · K562_BCR-2.fcs · K562_NTC-1.fcs · K562_NTC-2.fcs |
| Conditions | |
|---|---|
| · single stained-18s | K562_18s-1.fcs · K562_18s-2.fcs |
| · single stained-bcr | K562_BCR-1.fcs · K562_BCR-2.fcs |
| · single stained-abl | K562_ABL-1.fcs · K562_ABL-2.fcs |
| · 3-color multiplexed | K562_3 color-1.fcs · K562_3 color-2.fcs |
| · No Target Probe Control | K562_NTC-1.fcs · K562_NTC-2.fcs |
