Experiment Overview
| Repository ID: | FR-FCM-ZZC9 | Experiment name: | Temporal IFNg and CD4 mRNA and IFNg protein expression kinetics in stimulated PBMCs | MIFlowCyt score: | 96.57% |
| Primary researcher: | Chip Lomas | PI/manager: | Emily Park | Uploaded by: | Chip Lomas |
| Experiment dates: | 2014-01-20 - 2014-01-21 | Dataset uploaded: | May 2014 | Last updated: | Aug 2014 |
| Keywords: | [PBMC] [CD4] [Gene expression] [RNA Flow cytometry] | Manuscripts: | [25123411] |
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| Organizations: |
BD Biosciences, Research and Development, San Jose, CA (USA)
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| Purpose: | The purpose of the experiment was to explore the kinetics of CD4 mRNA, IFNg mRNA, and IFNg protein in PMA/Ionomycin stimulated PBMCs over 5 hours. | ||||
| Conclusion: | IFNg mRNA positive cells were observed at 30 min, reaching steady state by 120 min. INFg protein expression was first observed at 60 min, increasing at 120 min. Decline in CD4 mRNA expression became apparent after 120 min. | ||||
| Comments: | Figure 3 A,B and supplemental Figure 3 A,B: Simultaneous flow cytometric analysis of IFN-g and CD4 mRNA and protein expression kinetics in human peripheral blood mononuclear cells during activation | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Instrument was set up using CST in FACSDiva software. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · 0 min | RNA_T0-NTC_005.fcs · RNA_T0_006.fcs |
| · 10 min | RNA_T10-PMA+Ion_007.fcs |
| · 30 min | RNA_T30-PMA+Ion_008.fcs |
| · 60 min | RNA_T60-PMA+Ion_009.fcs |
| · 120 min | RNA_T120-PMA+Ion_010.fcs |
| · 300 min | RNA_T5-PMA+Ion-NTC_014.fcs · RNA_T5-PMA+Ion_011.fcs · RNA_T5-unstim-NTC_013.fcs · RNA_T5-unstim_012.fcs |
| Conditions | |
|---|---|
| · PMA/Ionomycin Stimulation | RNA_T10-PMA+Ion_007.fcs · RNA_T120-PMA+Ion_010.fcs · RNA_T30-PMA+Ion_008.fcs · RNA_T5-PMA+Ion-NTC_014.fcs · RNA_T5-PMA+Ion_011.fcs · RNA_T60-PMA+Ion_009.fcs |
| · Rested | RNA_T0-NTC_005.fcs · RNA_T0_006.fcs · RNA_T5-unstim-NTC_013.fcs · RNA_T5-unstim_012.fcs |
| Sample Type | |
|---|---|
| · Experimental Sample | RNA_T0_006.fcs · RNA_T10-PMA+Ion_007.fcs · RNA_T120-PMA+Ion_010.fcs · RNA_T30-PMA+Ion_008.fcs · RNA_T5-PMA+Ion_011.fcs · RNA_T5-unstim_012.fcs · RNA_T60-PMA+Ion_009.fcs |
| · No Target Probe added-negative sample | RNA_T0-NTC_005.fcs · RNA_T5-PMA+Ion-NTC_014.fcs · RNA_T5-unstim-NTC_013.fcs |
