Experiment Overview
| Repository ID: | FR-FCM-ZZCD | Experiment name: | RNA Flow Cytometry: Evaluation of RNA staining in suspension cells | MIFlowCyt score: | 95.75% |
| Primary researcher: | Chip Lomas | PI/manager: | Emily Park | Uploaded by: | Chip Lomas |
| Experiment dates: | 2012-12-12 - 2012-12-12 | Dataset uploaded: | May 2014 | Last updated: | May 2014 |
| Keywords: | [Gene expression] [RNA Amplification] [RNA hybridization] [RNA Flow cytometry] | Manuscripts: | [23441230] [PMC3575505] |
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| Organizations: |
BD Biosciences, Research and Development, San Jose, CA (USA)
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| Purpose: | The purpose of this experiment was to demonstrate that RNA staining could be achieved in suspension cells, with comparable specificity and sensitivity to previously established slide based staining method. | ||||
| Conclusion: | HIV+ cell populations were identified with expected frequencies using both suspension and slide staining methods. | ||||
| Comments: | Detection of Low Abundance RNA Molecules in Individual Cells by Flow Cytometry Figure 2a | ||||
| Funding: | Not disclosed | ||||
| Quality control: | instrument was setup with CST in FACSDiva software. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · No Target Probe Negative Control | 121212_IIIB NTC_001.fcs |
| · Stained Sample | 121212_IIIB_002.fcs |
