Annotation Details for Experiment:
Human early preterm and full term cord blood study
Repository ID: FR-FCM-ZZZA
Experiment Overview
| Repository ID: | FR-FCM-ZZZA | Experiment name: | Human early preterm and full term cord blood study | MIFlowCyt score: | 70.50% |
| Primary researcher: | Francesca D'Alessio | PI/manager: | Francesca D'Alessio | Uploaded by: | Francesca D'Alessio |
| Experiment dates: | 2007-03-01 - 2010-07-26 | Dataset uploaded: | Thu Aug 04 09:52:48 -0400 2011 | Last updated: | Sat Jan 05 14:09:21 -0500 2013 |
| Keywords: | [Human early preterm cord blood] [CD34] [hematopoietic stem cells] | Pubmed IDs: | [21182179] |
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| Organizations: |
Università degli Studi di Napoli Federico II, Napoli, N/A (Italy)
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| Purpose: | The aim of our study was to compare the immunophenotype of human early preterm cord blood samples with these of human full term cord blood specimens. The first step was the detection and anlysis of the most immature CD34+CD38- population detected in two aforementioned kind of samples. Therefore we analyzed the frequency of the newly identified CD34+CD243+ subset in both early preterm and full term cord blood. In conclusion, we studied the maturation pattern of B-cells in the two kind of samples of our interest. | ||||
| Conclusion: | As to CD38, we observed that early preterm samples were richer in CD34+CD45dimCD38- immature cells compared to fullterm specimen. Our data showed that CD34+CD45dim cells from early preterm cord blood samples displayed higher percentage of CD133+CD38- undifferentiated cells respect tp full term counterparts. We therefore found a higher percentage of pro-B and pre-B cells in early preterm samples than in full term cord blood samples. | ||||
| Comments: | These results evidence the hematopoietic peculiarity of early preterm cord blood samples, potentially useful for developing novel strategies of stem cell-based therapy. | ||||
| Quality control: | None | ||||
Flow Sample/Specimen Details
B-cells maturation in human Early Preterm Cord Blood.fcs :
| Description | Cord blood |
| Sample source: | [ biological ] Cord blood - Homo sapiens - 21 - 8 years - unknown - unknown - Ficoll-Paque gradient |
| Sample characteristic: | Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution. |
| Sample treatment: | none |
B-cells maturation in human Term Cord Blood.fcs :
| Description | Cord blood |
| Sample source: | [ biological ] Cord blood - Homo sapiens - 21 - 8 years - unknown - unknown - Ficoll-Paque gradient |
| Sample characteristic: | Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution. |
| Sample treatment: | Ficoll-Paque density gradient |
CD133+CD38- population in human Early Preterm Cord Blood.fcs :
| Description | Cord blood |
| Sample source: | [ biological ] Cord blood - Homo sapiens - 21 - 8 years - unknown - unknown - Ficoll-Paque gradient |
| Sample characteristic: | Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution. |
| Sample treatment: | Ficoll-Paque density gradient |
CD133+CD38- population in human term Cord Blood.fcs :
| Description | Cord blood |
| Sample source: | [ biological ] Cord blood - Homo sapiens - 21 - 8 years - unknown - unknown - Ficoll-Paque gradient |
| Sample characteristic: | Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution. |
| Sample treatment: | Ficoll-Paque density gradient |
CD34+CD38- and CD34+CD243+ populations in human Early Preterm Cord Blood.fcs :
| Description | Cord blood |
| Sample source: | [ biological ] Cord blood - Homo sapiens - 21 - 8 years - unknown - unknown - Ficoll-Paque gradient |
| Sample characteristic: | Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution. |
| Sample treatment: | Ficoll-Paque density gradient |
CD34+CD38- and CD34+CD243+ populations in human Term Cord Blood.fcs :
| Description | Cord blood |
| Sample source: | [ biological ] Cord blood - Homo sapiens - 21 - 8 years - unknown - unknown - Ficoll-Paque gradient |
| Sample characteristic: | Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution. |
| Sample treatment: | Ficoll-Paque density gradient |
Flow Cytometer Details
BD FACSCanto II, Becton Dickinson (BD Biosciences)
used for all 6 FCS files.
B-cells maturation in human Early Preterm Cord Blood.fcs
B-cells maturation in human Term Cord Blood.fcs
CD133+CD38- population in human Early Preterm Cord Blood.fcs
CD133+CD38- population in human term Cord Blood.fcs
CD34+CD38- and CD34+CD243+ populations in human Early Preterm Cord Blood.fcs
CD34+CD38- and CD34+CD243+ populations in human Term Cord Blood.fcs
B-cells maturation in human Early Preterm Cord Blood.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | BD FACSCanto II | |
| Optical filter installation dates |
B-cells maturation in human Term Cord Blood.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | BD FACSCanto II | |
| Optical filter installation dates |
CD133+CD38- population in human Early Preterm Cord Blood.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | BD FACSCanto II | |
| Optical filter installation dates |
CD133+CD38- population in human term Cord Blood.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | BD FACSCanto II | |
| Optical filter installation dates |
CD34+CD38- and CD34+CD243+ populations in human Early Preterm Cord Blood.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | BD FACSCanto II | |
| Optical filter installation dates |
CD34+CD38- and CD34+CD243+ populations in human Term Cord Blood.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | BD FACSCanto II | |
| Optical filter installation dates |
Data Analysis Details
No illustrations provided.