Annotation Details for Experiment:
Optimization of C11-BODIPY581/591 staining for lipid peroxidation in C. reinhardtii
Repository ID: FR-FCM-ZZ72
Experiment Overview
| Repository ID: | FR-FCM-ZZ72 | Experiment name: | Optimization of C11-BODIPY581/591 staining for lipid peroxidation in C. reinhardtii | MIFlowCyt score: | 46.40% |
| Primary researcher: | Giulia Cheloni | PI/manager: | Giulia Cheloni | Uploaded by: | Giulia Cheloni |
| Experiment dates: | 2012-04-02 - 2012-06-29 | Dataset uploaded: | Wed Jul 10 07:26:25 -0400 2013 | Last updated: | Thu Oct 24 16:35:03 -0400 2013 |
| Keywords: | [C11-BODIPY581/591; oxidative stress; lipid peroxidation; green algae; Chlamydomonas reinhardtii;] | Manuscripts: | [23943236] |
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| Organizations: |
Institut F.-A. Forel, University of Geneva, Versoix, (Switzerland)
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| Purpose: | The aim of this set of experiments was to optimize the C11-BODIPY581/591 staining conditions and to explore the capability of the dye to stain Chlamydomonas reinhardtii cells under various cell and dye concentrations and different staining procedures. | ||||
| Conclusion: | The dye resulted to be applicable to a wide range of staining procedures thanks to its high stability inside the cellular membrane and its specificity of reaction with oxyl-radicals, moreover its low interference with cell autofluorescence makes it a reliable dye for algal staining. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Accuri FCM calibration was periodically inspected following the manufacturer instructions during the entire work. Algal axenicity was repeatedly checked during the period of experiments. All experiments were performed at least three times as independent replicates | ||||
Flow Sample/Specimen Details
A1 C.reihardtii not exposed control t=0.fcs :
| Description | Chlamydomonas reinhardtii CPCC11 wild type, mid exponential growth phase, grown in 150 mL glass flasks containing 50 mL of TAPx4 medium and incubated under rotary shaking of 100 rpm, temperature of 20°C and illumination of 110 µmol phot m-2 s-1 |
| Sample source: | [ biological ] Chlamydomonas reinhardtii CPCC11 wild type, mid exponential growth phase - Chlamydomonas reinhardtii - 0 - N/A - N/A - N/A - grown in 150 mL glass flasks containing 50 mL of TAPx4 medium and incubated under rotary shaking of 100 rpm, temperature of 20°C and illumination of 110 µmol phot m-2 s-1 |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | sample was centrifuged and resuspended in exposure medium MES 10mM pH 7.0 + macronutrients |
A10 C.reihardtii exposed H2O2 5mM t=60min+C11-BODIPY581_591 30 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to H2O2 5mM 60 minutes and stained with 2.5 microM C11-BODIPY581/591 for 30 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A11 C.reihardtii exposed H2O2 5mM t=60min+C11-BODIPY581_591 60 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to H2O2 5mM for 60 minutes and stained with 2.5 microM C11-BODIPY581/591 for 60 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A12 C.reihardtii exposed H2O2 5mM t=48h.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to H2O2 5mM for 48 hours, |
| Sample source: | |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | none |
A13 C.reihardtii exposed H2O2 5mM t=48h+C11-BODIPY581_591 30 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to H2O2 5mM for 48 hours and stained with 2.5 microM C11-BODIPY581/591 for 30 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A14 C.reihardtii exposed H2O2 5mM t=48h+C11-BODIPY581_591 60 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to H2O2 5mM for 48 hours and stained with 2.5 microM C11-BODIPY581/591 for 60 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A15 C.reihardtii exposed visible+UV radiation t=0.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to visible+UV radiation |
| Sample source: | |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | none |
A16 C.reihardtii exposed visible+UV radiation t=60min.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to visible+UV irradiation for 60 min |
| Sample source: | |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | none |
A17 C.reihardtii exposed visible+UV radiation t=60min+C11-BODIPY581_591 30 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to visible+UV irradiation for 60 min and stained | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A18 C.reihardtii exposed visible+UV radiation t=60min+C11-BODIPY581_591 60 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to visible+UV irradiation for 60 min and stained with C11-BODIPY for 60 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A19 C.reihardtii exposed visible+UV radiation t=48h.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to visible+UV irradiation for 48h |
| Sample source: | |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | none |
A2 C.reihardtii not exposed control t=60min.fcs :
| Description | Chlamydomonas reinhardtii in experimental medium after 60 min incubation |
| Sample source: | |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | none |
A20 C.reihardtii exposed visible+UV radiation t=48h+C11-BODIPY581_591 30 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to visible+UV irradiation for 48 h and stained with 2.5 microM C11-BODIPY581/591 for 30 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A21 C.reihardtii exposed visible+UV radiation t=48h+C11-BODIPY581_591 60 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to visible+UV irradiation for 48 h and stained with 2.5 microM C11-BODIPY581/591 for 60 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A22 C.reihardtii exposed DMSO 0.25% t=0.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to DMSO 0.25% |
| Sample source: | |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | none |
A23 C.reihardtii exposed DMSO 0.25% t=60min.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to DMSO 0.25% for 60 minutes |
| Sample source: | |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | none |
A24 C.reihardtii exposed DMSO0.25% t=60min +C11-BODIPY581_591 30 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to DMSO 0.25% for 60 minutes and stained with 2.5 microM C11-BODIPY581/591 for 30 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A25 C.reihardtii exposed DMSO0.25% t=60min +C11-BODIPY581_591 60 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to DMSO 0.25% for 60 min and stained with 2.5 microM C11-BODIPY581/591 for 60 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A26 C.reihardtii exposed DMSO 0.25% t=48h.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to DMSO 0.25% for 48 hours |
| Sample source: | |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | none |
A27 C.reihardtii exposed DMSO0.25% t=48h +C11-BODIPY581_591 30 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium, exposed to DMSO 0.25% for 48 hours and stained with 2.5 microM C11-BODIPY581/591 for 30 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A28 C.reihardtii exposed DMSO0.25% t=48h +C11-BODIPY581_591 60 min staining.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium, exposed to DMSO 0.25% for 48 hours and stained with 2.5 microM C11-BODIPY581/591 for 60 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A3 C.reihardtii not exposed control t=60min +C11-BODIPY581_591 30 min staining.fcs :
| Description | Chlamydomonas reinhardtii in experimental medium after 60 min incubation stained with 2.5 microM C11-BODIPY581/591 for 30 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A4 C.reihardtii not exposed control t=60min +C11-BODIPY581_591 60 min staining.fcs :
| Description | Chlamydomonas reinhardtii in experimental medium after 60 min incubation stained with 2.5 microM C11-BODIPY581/591 for 60 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A5 C.reihardtii not exposed control t=48h +C11-BODIPY581_591 30 min staining.fcs :
| Description | Chlamydomonas reinhardtii in experimental medium after 48h incubation and stained with 2.5 microM C11-BODIPY581/591 for 30 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A6 C.reihardtii not exposed control t=48h +C11-BODIPY581_591 60 min staining.fcs :
| Description | Chlamydomonas reinhardtii in experimental medium after 48h incubation and stained with 2.5 microM C11-BODIPY581/591 for 60 minutes | ||||||||||||||||
| Sample source: | |||||||||||||||||
| Sample characteristic: | Chlamydomonas cells | ||||||||||||||||
| Sample treatment: | Single stain | ||||||||||||||||
| Staining: |
Individual reagents
|
A7 C.reihardtii not exposed control t=48h.fcs :
| Description | Chlamydomonas reinhardtii in experimental medium after 48 hour incubation |
| Sample source: | |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | none |
A8 C.reihardtii exposed H2O2 5mM t=0.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to H2O2 |
| Sample source: | |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | none |
A9 C.reihardtii exposed H2O2 5mM t=60min.fcs :
| Description | the culture in mid exponential growth phase was washed, resuspended in exposure medium and exposed to H2O2 5mM for 60 min |
| Sample source: | |
| Sample characteristic: | Chlamydomonas cells |
| Sample treatment: | none |
B1 C.reinhardtii H2O2 5mM not stained.fcs :
No sample annotation exists for this FCS file.
B2 C.reinhardtii H2O2 5mM_washed before staining 2.5 microM C11-BODIPY581_591.fcs :
No sample annotation exists for this FCS file.
B3 C.reinhardtii H2O2 5mM_ stained 2.5 microM C11-BODIPY581_591 .fcs :
No sample annotation exists for this FCS file.
C1 C.reinhardtii H2O2 not stained cell density 10^4 .fcs :
No sample annotation exists for this FCS file.
C10 C.reinhardtii H2O2 1.25 microM C11-BODIPY581_591 cell density 10^6 .fcs :
No sample annotation exists for this FCS file.
C11 C.reinhardtii H2O2 2.5 microM C11-BODIPY581_591 cell density 10^6 .fcs :
No sample annotation exists for this FCS file.
C12 C.reinhardtii H2O2 +5 microM C11-BODIPY581_591 cell density 10^6 .fcs :
No sample annotation exists for this FCS file.
C2 C.reinhardtii H2O2 1.25 microM C11-BODIPY581_591 cell density 10^4.fcs :
No sample annotation exists for this FCS file.
C3 C.reinhardtii H2O2 2.5 microM C11-BODIPY581_591 cell density 10^4.fcs :
No sample annotation exists for this FCS file.
C4 C.reinhardtii H2O2 5 microM C11-BODIPY581_591 cell density 10^4.fcs :
No sample annotation exists for this FCS file.
C5C.reinhardtii H2O2 not stained cell density 10^5 .fcs :
No sample annotation exists for this FCS file.
C6 C.reinhardtii H2O2 1.25 microM C11-BODIPY581_591 cell density 10^5.fcs :
No sample annotation exists for this FCS file.
C7 C.reinhardtii H2O2 2.5 microM C11-BODIPY581_591 cell density 10^5.fcs :
No sample annotation exists for this FCS file.
C8 C.reinhardtii H2O2 5 microM C11-BODIPY581_591 cell density 10^5.fcs :
No sample annotation exists for this FCS file.
C9 C.reinhardtii H2O2 not stained cell density 10^6 .fcs :
No sample annotation exists for this FCS file.
D1 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=0.fcs :
No sample annotation exists for this FCS file.
D10 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=0.fcs :
No sample annotation exists for this FCS file.
D11 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=5.fcs :
No sample annotation exists for this FCS file.
D12 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=10.fcs :
No sample annotation exists for this FCS file.
D13C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=15.fcs :
No sample annotation exists for this FCS file.
D14 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=20.fcs :
No sample annotation exists for this FCS file.
D15 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=25.fcs :
No sample annotation exists for this FCS file.
D16 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=30.fcs :
No sample annotation exists for this FCS file.
D17 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=45.fcs :
No sample annotation exists for this FCS file.
D18 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=60.fcs :
No sample annotation exists for this FCS file.
D19 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=0.fcs :
No sample annotation exists for this FCS file.
D2 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=5.fcs :
No sample annotation exists for this FCS file.
D20 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=5.fcs :
No sample annotation exists for this FCS file.
D21 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=10.fcs :
No sample annotation exists for this FCS file.
D22 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=15.fcs :
No sample annotation exists for this FCS file.
D23 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=20.fcs :
No sample annotation exists for this FCS file.
D24 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=25.fcs :
No sample annotation exists for this FCS file.
D25 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=30.fcs :
No sample annotation exists for this FCS file.
D26 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=45.fcs :
No sample annotation exists for this FCS file.
D27 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=60.fcs :
No sample annotation exists for this FCS file.
D28 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=0.fcs :
No sample annotation exists for this FCS file.
D29 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=5.fcs :
No sample annotation exists for this FCS file.
D3 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=10.fcs :
No sample annotation exists for this FCS file.
D30 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=10.fcs :
No sample annotation exists for this FCS file.
D31 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=15.fcs :
No sample annotation exists for this FCS file.
D32 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=20.fcs :
No sample annotation exists for this FCS file.
D33 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=25.fcs :
No sample annotation exists for this FCS file.
D34 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=30.fcs :
No sample annotation exists for this FCS file.
D34 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=45.fcs :
No sample annotation exists for this FCS file.
D35 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=60.fcs :
No sample annotation exists for this FCS file.
D4 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=15.fcs :
No sample annotation exists for this FCS file.
D5 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=20.fcs :
No sample annotation exists for this FCS file.
D6 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=25.fcs :
No sample annotation exists for this FCS file.
D7 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=30.fcs :
No sample annotation exists for this FCS file.
D8 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=45.fcs :
No sample annotation exists for this FCS file.
D9 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=60.fcs :
No sample annotation exists for this FCS file.
Flow Cytometer Details
Accuri C6, Becton Dickinson (BD Biosciences)
used for all 79 FCS files.
A1 C.reihardtii not exposed control t=0.fcs
A10 C.reihardtii exposed H2O2 5mM t=60min+C11-BODIPY581_591 30 min staining.fcs
A11 C.reihardtii exposed H2O2 5mM t=60min+C11-BODIPY581_591 60 min staining.fcs
A12 C.reihardtii exposed H2O2 5mM t=48h.fcs
A13 C.reihardtii exposed H2O2 5mM t=48h+C11-BODIPY581_591 30 min staining.fcs
A14 C.reihardtii exposed H2O2 5mM t=48h+C11-BODIPY581_591 60 min staining.fcs
A15 C.reihardtii exposed visible+UV radiation t=0.fcs
A16 C.reihardtii exposed visible+UV radiation t=60min.fcs
A17 C.reihardtii exposed visible+UV radiation t=60min+C11-BODIPY581_591 30 min staining.fcs
A18 C.reihardtii exposed visible+UV radiation t=60min+C11-BODIPY581_591 60 min staining.fcs
A19 C.reihardtii exposed visible+UV radiation t=48h.fcs
A2 C.reihardtii not exposed control t=60min.fcs
A20 C.reihardtii exposed visible+UV radiation t=48h+C11-BODIPY581_591 30 min staining.fcs
A21 C.reihardtii exposed visible+UV radiation t=48h+C11-BODIPY581_591 60 min staining.fcs
A22 C.reihardtii exposed DMSO 0.25% t=0.fcs
A23 C.reihardtii exposed DMSO 0.25% t=60min.fcs
A24 C.reihardtii exposed DMSO0.25% t=60min +C11-BODIPY581_591 30 min staining.fcs
A25 C.reihardtii exposed DMSO0.25% t=60min +C11-BODIPY581_591 60 min staining.fcs
A26 C.reihardtii exposed DMSO 0.25% t=48h.fcs
A27 C.reihardtii exposed DMSO0.25% t=48h +C11-BODIPY581_591 30 min staining.fcs
A28 C.reihardtii exposed DMSO0.25% t=48h +C11-BODIPY581_591 60 min staining.fcs
A3 C.reihardtii not exposed control t=60min +C11-BODIPY581_591 30 min staining.fcs
A4 C.reihardtii not exposed control t=60min +C11-BODIPY581_591 60 min staining.fcs
A5 C.reihardtii not exposed control t=48h +C11-BODIPY581_591 30 min staining.fcs
A6 C.reihardtii not exposed control t=48h +C11-BODIPY581_591 60 min staining.fcs
A7 C.reihardtii not exposed control t=48h.fcs
A8 C.reihardtii exposed H2O2 5mM t=0.fcs
A9 C.reihardtii exposed H2O2 5mM t=60min.fcs
B1 C.reinhardtii H2O2 5mM not stained.fcs
B2 C.reinhardtii H2O2 5mM_washed before staining 2.5 microM C11-BODIPY581_591.fcs
B3 C.reinhardtii H2O2 5mM_ stained 2.5 microM C11-BODIPY581_591 .fcs
C1 C.reinhardtii H2O2 not stained cell density 10^4 .fcs
C10 C.reinhardtii H2O2 1.25 microM C11-BODIPY581_591 cell density 10^6 .fcs
C11 C.reinhardtii H2O2 2.5 microM C11-BODIPY581_591 cell density 10^6 .fcs
C12 C.reinhardtii H2O2 +5 microM C11-BODIPY581_591 cell density 10^6 .fcs
C2 C.reinhardtii H2O2 1.25 microM C11-BODIPY581_591 cell density 10^4.fcs
C3 C.reinhardtii H2O2 2.5 microM C11-BODIPY581_591 cell density 10^4.fcs
C4 C.reinhardtii H2O2 5 microM C11-BODIPY581_591 cell density 10^4.fcs
C5C.reinhardtii H2O2 not stained cell density 10^5 .fcs
C6 C.reinhardtii H2O2 1.25 microM C11-BODIPY581_591 cell density 10^5.fcs
C7 C.reinhardtii H2O2 2.5 microM C11-BODIPY581_591 cell density 10^5.fcs
C8 C.reinhardtii H2O2 5 microM C11-BODIPY581_591 cell density 10^5.fcs
C9 C.reinhardtii H2O2 not stained cell density 10^6 .fcs
D1 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=0.fcs
D10 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=0.fcs
D11 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=5.fcs
D12 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=10.fcs
D13C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=15.fcs
D14 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=20.fcs
D15 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=25.fcs
D16 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=30.fcs
D17 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=45.fcs
D18 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=60.fcs
D19 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=0.fcs
D2 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=5.fcs
D20 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=5.fcs
D21 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=10.fcs
D22 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=15.fcs
D23 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=20.fcs
D24 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=25.fcs
D25 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=30.fcs
D26 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=45.fcs
D27 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=60.fcs
D28 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=0.fcs
D29 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=5.fcs
D3 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=10.fcs
D30 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=10.fcs
D31 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=15.fcs
D32 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=20.fcs
D33 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=25.fcs
D34 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=30.fcs
D34 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=45.fcs
D35 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=60.fcs
D4 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=15.fcs
D5 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=20.fcs
D6 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=25.fcs
D7 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=30.fcs
D8 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=45.fcs
D9 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=60.fcs
A1 C.reihardtii not exposed control t=0.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A10 C.reihardtii exposed H2O2 5mM t=60min+C11-BODIPY581_591 30 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A11 C.reihardtii exposed H2O2 5mM t=60min+C11-BODIPY581_591 60 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A12 C.reihardtii exposed H2O2 5mM t=48h.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A13 C.reihardtii exposed H2O2 5mM t=48h+C11-BODIPY581_591 30 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A14 C.reihardtii exposed H2O2 5mM t=48h+C11-BODIPY581_591 60 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A15 C.reihardtii exposed visible+UV radiation t=0.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A16 C.reihardtii exposed visible+UV radiation t=60min.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A17 C.reihardtii exposed visible+UV radiation t=60min+C11-BODIPY581_591 30 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A18 C.reihardtii exposed visible+UV radiation t=60min+C11-BODIPY581_591 60 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A19 C.reihardtii exposed visible+UV radiation t=48h.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A2 C.reihardtii not exposed control t=60min.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A20 C.reihardtii exposed visible+UV radiation t=48h+C11-BODIPY581_591 30 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A21 C.reihardtii exposed visible+UV radiation t=48h+C11-BODIPY581_591 60 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A22 C.reihardtii exposed DMSO 0.25% t=0.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A23 C.reihardtii exposed DMSO 0.25% t=60min.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A24 C.reihardtii exposed DMSO0.25% t=60min +C11-BODIPY581_591 30 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A25 C.reihardtii exposed DMSO0.25% t=60min +C11-BODIPY581_591 60 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A26 C.reihardtii exposed DMSO 0.25% t=48h.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A27 C.reihardtii exposed DMSO0.25% t=48h +C11-BODIPY581_591 30 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A28 C.reihardtii exposed DMSO0.25% t=48h +C11-BODIPY581_591 60 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A3 C.reihardtii not exposed control t=60min +C11-BODIPY581_591 30 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A4 C.reihardtii not exposed control t=60min +C11-BODIPY581_591 60 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A5 C.reihardtii not exposed control t=48h +C11-BODIPY581_591 30 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A6 C.reihardtii not exposed control t=48h +C11-BODIPY581_591 60 min staining.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A7 C.reihardtii not exposed control t=48h.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A8 C.reihardtii exposed H2O2 5mM t=0.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
A9 C.reihardtii exposed H2O2 5mM t=60min.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
B1 C.reinhardtii H2O2 5mM not stained.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
B2 C.reinhardtii H2O2 5mM_washed before staining 2.5 microM C11-BODIPY581_591.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
B3 C.reinhardtii H2O2 5mM_ stained 2.5 microM C11-BODIPY581_591 .fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C1 C.reinhardtii H2O2 not stained cell density 10^4 .fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C10 C.reinhardtii H2O2 1.25 microM C11-BODIPY581_591 cell density 10^6 .fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C11 C.reinhardtii H2O2 2.5 microM C11-BODIPY581_591 cell density 10^6 .fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C12 C.reinhardtii H2O2 +5 microM C11-BODIPY581_591 cell density 10^6 .fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C2 C.reinhardtii H2O2 1.25 microM C11-BODIPY581_591 cell density 10^4.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C3 C.reinhardtii H2O2 2.5 microM C11-BODIPY581_591 cell density 10^4.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C4 C.reinhardtii H2O2 5 microM C11-BODIPY581_591 cell density 10^4.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C5C.reinhardtii H2O2 not stained cell density 10^5 .fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C6 C.reinhardtii H2O2 1.25 microM C11-BODIPY581_591 cell density 10^5.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C7 C.reinhardtii H2O2 2.5 microM C11-BODIPY581_591 cell density 10^5.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C8 C.reinhardtii H2O2 5 microM C11-BODIPY581_591 cell density 10^5.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
C9 C.reinhardtii H2O2 not stained cell density 10^6 .fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D1 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=0.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D10 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=0.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D11 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=5.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D12 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=10.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D13C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=15.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D14 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=20.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D15 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=25.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D16 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=30.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D17 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=45.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D18 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 1 mM t=60.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D19 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=0.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D2 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=5.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D20 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=5.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D21 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=10.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D22 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=15.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D23 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=20.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D24 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=25.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D25 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=30.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D26 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=45.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D27 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 5 mM t=60.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D28 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=0.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D29 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=5.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D3 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=10.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D30 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=10.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D31 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=15.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D32 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=20.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D33 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=25.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D34 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=30.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D34 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=45.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D35 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591+H2O2 10 mM t=60.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D4 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=15.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D5 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=20.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D6 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=25.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D7 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=30.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D8 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=45.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
D9 C.reinhardtii preloaded 2.5 microM C11-BODIPY581_591 t=60.fcs
| Manufacturer | Becton Dickinson (BD Biosciences) | Default instrument settings used. |
|---|---|---|
| Model | Accuri C6 | |
| Optical filter installation dates |
Data Analysis Details
No illustrations provided.