Experiment Overview

Repository ID: FR-FCM-ZZZ3 Experiment name: Identification of B cells through negative gating MIFlowCyt score: 89.83%
Primary researcher: Karin Breuer PI/manager: Karin Breuer Uploaded by: Karin Breuer
Experiment dates: 2007-05-30 - 2007-08-21 Dataset uploaded: Mon Apr 18 17:36:25 -0400 2011 Last updated: Tue Jan 15 12:54:42 -0500 2013
Keywords: [Innate Immune Response] [Toll-like receptors] [Activation markers] [B cells] [MIFlowCyt] Manuscripts: [20131398] [PMC2878765] Cytalogo
Organizations: Child & Family Research Institute, Department of Pediatrics, Vancouver, BC (Canada)
University of Washington Medical Center, Department of Immunology, Seattle, Washington (USA)
Purpose: The purpose of the experiment presented here was to test whether human B cells can be identified through a negative-gating strategy.
Conclusion: B cells can be identified through a negative-gating strategy. Specifically, B lymphocytes are the MHC II+, CD14neg, CD11cneg, and CD123neg cells. Only on the extreme ends of a response spectrum (i.e., either none of the B cells respond to a given innate stimulation or all/most B cells respond but not monocyte, mDC or pDC) would this B cell negative-gating strategy supply acceptable data. In other words, our negative-gating strategy would potentially be difficult to interpret if both B cell and non-B cell populations responded in low frequency.
Comments: We set out to compare a negative-gating strategy to identify B cells with the usual positive identification of these cells using the canonical B cell surface marker, CD19. We also wanted to assess the functional response of these B cells to Toll-like receptor stimulation using the following TLR ligands: PAM3CSK4, LPS, and CpG-A ODN 2336. The blood draws, PBMC isolation, and TLR stimulations were performed between 30 May and 5 June 2007. The flow cytometry analyses were performed between 8 June and 21 August 2007.
Funding: Not disclosed
Quality control: To standardize voltage settings across samples acquired on different days, single stained controls were included. Voltages were adjusted such that fluorescence intensity was identical for each antibody, regardless of date of acquisition.


Experiment variables

Individuals
- a2006 a2006_O1T2pb05i_A1_A01.fcs - a2006_O1T2pb05i_A2_A02.fcs - a2006_O1T2pb05i_A3_A03.fcs - a2006_O1T2pb05i_A4_A04.fcs - a2006_O1T2pb05i_A5_A05.fcs - a2006_O1T2pb05i_A6_A06.fcs - a2006_O1T2pb05i_A7_A07.fcs - a2006_O1T2pb05i_A8_A08.fcs - a2006_O1T2pb05i_B1_B01.fcs - a2006_O1T2pb05i_B2_B02.fcs - a2006_O1T2pb05i_B3_B03.fcs - a2006_O1T2pb05i_B4_B04.fcs - a2006_O1T2pb05i_B5_B05.fcs - a2006_O1T2pb05i_B6_B06.fcs - a2006_O1T2pb05i_B7_B07.fcs - a2006_O1T2pb05i_B8_B08.fcs - a2006_O1T2pb05i_C1_C01.fcs - a2006_O1T2pb05i_C2_C02.fcs - a2006_O1T2pb05i_C3_C03.fcs - a2006_O1T2pb05i_C4_C04.fcs - a2006_O1T2pb05i_C5_C05.fcs - a2006_O1T2pb05i_C6_C06.fcs - a2006_O1T2pb05i_C7_C07.fcs - a2006_O1T2pb05i_C8_C08.fcs - a2006_O1T2pb05i_D1_D01.fcs - a2006_O1T2pb05i_D2_D02.fcs - a2006_O1T2pb05i_D3_D03.fcs - a2006_O1T2pb05i_D4_D04.fcs - a2006_O1T2pb05i_D5_D05.fcs - a2006_O1T2pb05i_D6_D06.fcs - a2006_O1T2pb05i_D7_D07.fcs - a2006_O1T2pb05i_D8_D08.fcs - a2006_O1T2pb05i_E1_E01.fcs - a2006_O1T2pb05i_E2_E02.fcs - a2006_O1T2pb05i_E3_E03.fcs - a2006_O1T2pb05i_E4_E04.fcs - a2006_O1T2pb05i_E5_E05.fcs - a2006_O1T2pb05i_E6_E06.fcs - a2006_O1T2pb05i_E7_E07.fcs - a2006_O1T2pb05i_E8_E08.fcs - a2006_O1T2pb05i_F1_F01.fcs - a2006_O1T2pb05i_F2_F02.fcs - a2006_O1T2pb05i_F3_F03.fcs - a2006_O1T2pb05i_F4_F04.fcs - a2006_O1T2pb05i_controls_G10_G10.fcs - a2006_O1T2pb05i_controls_G11_G11.fcs - a2006_O1T2pb05i_controls_G12_G12.fcs - a2006_O1T2pb05i_controls_G1_G01.fcs - a2006_O1T2pb05i_controls_G2_G02.fcs - a2006_O1T2pb05i_controls_G3_G03.fcs - a2006_O1T2pb05i_controls_G4_G04.fcs - a2006_O1T2pb05i_controls_G5_G05.fcs - a2006_O1T2pb05i_controls_G6_G06.fcs - a2006_O1T2pb05i_controls_G7_G07.fcs - a2006_O1T2pb05i_controls_G8_G08.fcs - a2006_O1T2pb05i_controls_G9_G09.fcs - a2006_O1T2pb05i_controls_H1_H01.fcs - a2006_O1T2pb05i_controls_H2_H02.fcs - a2006_O1T2pb05i_controls_H3_H03.fcs
- a2007 a2007_O1T2pb05i_A1_A01.fcs - a2007_O1T2pb05i_A2_A02.fcs - a2007_O1T2pb05i_A3_A03.fcs - a2007_O1T2pb05i_A4_A04.fcs - a2007_O1T2pb05i_A5_A05.fcs - a2007_O1T2pb05i_A6_A06.fcs - a2007_O1T2pb05i_A7_A07.fcs - a2007_O1T2pb05i_A8_A08.fcs - a2007_O1T2pb05i_B1_B01.fcs - a2007_O1T2pb05i_B2_B02.fcs - a2007_O1T2pb05i_B3_B03.fcs - a2007_O1T2pb05i_B4_B04.fcs - a2007_O1T2pb05i_B5_B05.fcs - a2007_O1T2pb05i_B6_B06.fcs - a2007_O1T2pb05i_B7_B07.fcs - a2007_O1T2pb05i_B8_B08.fcs - a2007_O1T2pb05i_C1_C01.fcs - a2007_O1T2pb05i_C2_C02.fcs - a2007_O1T2pb05i_C3_C03.fcs - a2007_O1T2pb05i_C4_C04.fcs - a2007_O1T2pb05i_C5_C05.fcs - a2007_O1T2pb05i_C6_C06.fcs - a2007_O1T2pb05i_C7_C07.fcs - a2007_O1T2pb05i_C8_C08.fcs - a2007_O1T2pb05i_D1_D01.fcs - a2007_O1T2pb05i_D2_D02.fcs - a2007_O1T2pb05i_D3_D03.fcs - a2007_O1T2pb05i_D4_D04.fcs - a2007_O1T2pb05i_D5_D05.fcs - a2007_O1T2pb05i_D6_D06.fcs - a2007_O1T2pb05i_D7_D07.fcs - a2007_O1T2pb05i_D8_D08.fcs - a2007_O1T2pb05i_E1_E01.fcs - a2007_O1T2pb05i_E2_E02.fcs - a2007_O1T2pb05i_E3_E03.fcs - a2007_O1T2pb05i_E4_E04.fcs - a2007_O1T2pb05i_E5_E05.fcs - a2007_O1T2pb05i_E6_E06.fcs - a2007_O1T2pb05i_E7_E07.fcs - a2007_O1T2pb05i_E8_E08.fcs - a2007_O1T2pb05i_F1_F01.fcs - a2007_O1T2pb05i_F2_F02.fcs - a2007_O1T2pb05i_controls_G10_G10.fcs - a2007_O1T2pb05i_controls_G11_G11.fcs - a2007_O1T2pb05i_controls_G12_G12.fcs - a2007_O1T2pb05i_controls_G1_G01.fcs - a2007_O1T2pb05i_controls_G2_G02.fcs - a2007_O1T2pb05i_controls_G3_G03.fcs - a2007_O1T2pb05i_controls_G4_G04.fcs - a2007_O1T2pb05i_controls_G5_G05.fcs - a2007_O1T2pb05i_controls_G6_G06.fcs - a2007_O1T2pb05i_controls_G7_G07.fcs - a2007_O1T2pb05i_controls_G8_G08.fcs - a2007_O1T2pb05i_controls_G9_G09.fcs - a2007_O1T2pb05i_controls_H1_H01.fcs - a2007_O1T2pb05i_controls_H2_H02.fcs - a2007_O1T2pb05i_controls_H3_H03.fcs
- a2008 a2008_O1T2pb05i_A1_A01.fcs - a2008_O1T2pb05i_A2_A02.fcs - a2008_O1T2pb05i_A3_A03.fcs - a2008_O1T2pb05i_A4_A04.fcs - a2008_O1T2pb05i_A5_A05.fcs - a2008_O1T2pb05i_A6_A06.fcs - a2008_O1T2pb05i_A7_A07.fcs - a2008_O1T2pb05i_A8_A08.fcs - a2008_O1T2pb05i_B1_B01.fcs - a2008_O1T2pb05i_B2_B02.fcs - a2008_O1T2pb05i_B3_B03.fcs - a2008_O1T2pb05i_B4_B04.fcs - a2008_O1T2pb05i_B5_B05.fcs - a2008_O1T2pb05i_B6_B06.fcs - a2008_O1T2pb05i_B7_B07.fcs - a2008_O1T2pb05i_B8_B08.fcs - a2008_O1T2pb05i_C1_C01.fcs - a2008_O1T2pb05i_C2_C02.fcs - a2008_O1T2pb05i_C3_C03.fcs - a2008_O1T2pb05i_C4_C04.fcs - a2008_O1T2pb05i_C5_C05.fcs - a2008_O1T2pb05i_C6_C06.fcs - a2008_O1T2pb05i_C7_C07.fcs - a2008_O1T2pb05i_C8_C08.fcs - a2008_O1T2pb05i_D1_D01.fcs - a2008_O1T2pb05i_D2_D02.fcs - a2008_O1T2pb05i_D3_D03.fcs - a2008_O1T2pb05i_D4_D04.fcs - a2008_O1T2pb05i_D5_D05.fcs - a2008_O1T2pb05i_D6_D06.fcs - a2008_O1T2pb05i_D7_D07.fcs - a2008_O1T2pb05i_D8_D08.fcs - a2008_O1T2pb05i_E1_E01.fcs - a2008_O1T2pb05i_E2_E02.fcs - a2008_O1T2pb05i_E3_E03.fcs - a2008_O1T2pb05i_E4_E04.fcs - a2008_O1T2pb05i_E5_E05.fcs - a2008_O1T2pb05i_E6_E06.fcs - a2008_O1T2pb05i_E7_E07.fcs - a2008_O1T2pb05i_E8_E08.fcs - a2008_O1T2pb05i_F1_F01.fcs - a2008_O1T2pb05i_F2_F02.fcs - a2008_O1T2pb05i_controls_G10_G10.fcs - a2008_O1T2pb05i_controls_G11_G11.fcs - a2008_O1T2pb05i_controls_G12_G12.fcs - a2008_O1T2pb05i_controls_G1_G01.fcs - a2008_O1T2pb05i_controls_G2_G02.fcs - a2008_O1T2pb05i_controls_G3_G03.fcs - a2008_O1T2pb05i_controls_G4_G04.fcs - a2008_O1T2pb05i_controls_G5_G05.fcs - a2008_O1T2pb05i_controls_G6_G06.fcs - a2008_O1T2pb05i_controls_G7_G07.fcs - a2008_O1T2pb05i_controls_G8_G08.fcs - a2008_O1T2pb05i_controls_G9_G09.fcs - a2008_O1T2pb05i_controls_H1_H01.fcs - a2008_O1T2pb05i_controls_H2_H02.fcs - a2008_O1T2pb05i_controls_H3_H03.fcs
- a2009 a2009_O1T2pb05i_A1_A01.fcs - a2009_O1T2pb05i_A2_A02.fcs - a2009_O1T2pb05i_A3_A03.fcs - a2009_O1T2pb05i_A4_A04.fcs - a2009_O1T2pb05i_A5_A05.fcs - a2009_O1T2pb05i_A6_A06.fcs - a2009_O1T2pb05i_A7_A07.fcs - a2009_O1T2pb05i_A8_A08.fcs - a2009_O1T2pb05i_B1_B01.fcs - a2009_O1T2pb05i_B2_B02.fcs - a2009_O1T2pb05i_B3_B03.fcs - a2009_O1T2pb05i_B4_B04.fcs - a2009_O1T2pb05i_B5_B05.fcs - a2009_O1T2pb05i_B6_B06.fcs - a2009_O1T2pb05i_B7_B07.fcs - a2009_O1T2pb05i_B8_B08.fcs - a2009_O1T2pb05i_C1_C01.fcs - a2009_O1T2pb05i_C2_C02.fcs - a2009_O1T2pb05i_C3_C03.fcs - a2009_O1T2pb05i_C4_C04.fcs - a2009_O1T2pb05i_C5_C05.fcs - a2009_O1T2pb05i_C6_C06.fcs - a2009_O1T2pb05i_C7_C07.fcs - a2009_O1T2pb05i_C8_C08.fcs - a2009_O1T2pb05i_D1_D01.fcs - a2009_O1T2pb05i_D2_D02.fcs - a2009_O1T2pb05i_D3_D03.fcs - a2009_O1T2pb05i_D4_D04.fcs - a2009_O1T2pb05i_D5_D05.fcs - a2009_O1T2pb05i_D6_D06.fcs - a2009_O1T2pb05i_D7_D07.fcs - a2009_O1T2pb05i_D8_D08.fcs - a2009_O1T2pb05i_E1_E01.fcs - a2009_O1T2pb05i_E2_E02.fcs - a2009_O1T2pb05i_E3_E03.fcs - a2009_O1T2pb05i_E4_E04.fcs - a2009_O1T2pb05i_E5_E05.fcs - a2009_O1T2pb05i_E6_E06.fcs - a2009_O1T2pb05i_E7_E07.fcs - a2009_O1T2pb05i_E8_E08.fcs - a2009_O1T2pb05i_F1_F01.fcs - a2009_O1T2pb05i_F2_F02.fcs - a2009_O1T2pb05i_controls_G10_G10.fcs - a2009_O1T2pb05i_controls_G11_G11.fcs - a2009_O1T2pb05i_controls_G12_G12.fcs - a2009_O1T2pb05i_controls_G1_G01.fcs - a2009_O1T2pb05i_controls_G2_G02.fcs - a2009_O1T2pb05i_controls_G3_G03.fcs - a2009_O1T2pb05i_controls_G4_G04.fcs - a2009_O1T2pb05i_controls_G5_G05.fcs - a2009_O1T2pb05i_controls_G6_G06.fcs - a2009_O1T2pb05i_controls_G7_G07.fcs - a2009_O1T2pb05i_controls_G8_G08.fcs - a2009_O1T2pb05i_controls_G9_G09.fcs - a2009_O1T2pb05i_controls_H1_H01.fcs - a2009_O1T2pb05i_controls_H2_H02.fcs - a2009_O1T2pb05i_controls_H3_H03.fcs
- a2010 a2010_O1T2pb05i_A1_A01.fcs - a2010_O1T2pb05i_A2_A02.fcs - a2010_O1T2pb05i_A3_A03.fcs - a2010_O1T2pb05i_A4_A04.fcs - a2010_O1T2pb05i_A8_A08.fcs - a2010_O1T2pb05i_B1_B01.fcs - a2010_O1T2pb05i_B2_B02.fcs - a2010_O1T2pb05i_B3_B03.fcs - a2010_O1T2pb05i_B4_B04.fcs - a2010_O1T2pb05i_B5_B05.fcs - a2010_O1T2pb05i_B6_B06.fcs - a2010_O1T2pb05i_B7_B07.fcs - a2010_O1T2pb05i_B8_B08.fcs - a2010_O1T2pb05i_C1_C01.fcs - a2010_O1T2pb05i_C2_C02.fcs - a2010_O1T2pb05i_C3_C03.fcs - a2010_O1T2pb05i_C4_C04.fcs - a2010_O1T2pb05i_C5_C05.fcs - a2010_O1T2pb05i_C6_C06.fcs - a2010_O1T2pb05i_C7_C07.fcs - a2010_O1T2pb05i_C8_C08.fcs - a2010_O1T2pb05i_D1_D01.fcs - a2010_O1T2pb05i_D2_D02.fcs - a2010_O1T2pb05i_D3_D03.fcs - a2010_O1T2pb05i_D4_D04.fcs - a2010_O1T2pb05i_D5_D05.fcs - a2010_O1T2pb05i_D6_D06.fcs - a2010_O1T2pb05i_D7_D07.fcs - a2010_O1T2pb05i_D8_D08.fcs - a2010_O1T2pb05i_E1_E01.fcs - a2010_O1T2pb05i_E2_E02.fcs - a2010_O1T2pb05i_E3_E03.fcs - a2010_O1T2pb05i_E4_E04.fcs - a2010_O1T2pb05i_E5_E05.fcs - a2010_O1T2pb05i_E6_E06.fcs - a2010_O1T2pb05i_E7_E07.fcs - a2010_O1T2pb05i_E8_E08.fcs - a2010_O1T2pb05i_F1_F01.fcs - a2010_O1T2pb05i_F2_F02.fcs - a2010_O1T2pb05i_controls_G10_G10.fcs - a2010_O1T2pb05i_controls_G11_G11.fcs - a2010_O1T2pb05i_controls_G12_G12.fcs - a2010_O1T2pb05i_controls_G1_G01.fcs - a2010_O1T2pb05i_controls_G2_G02.fcs - a2010_O1T2pb05i_controls_G3_G03.fcs - a2010_O1T2pb05i_controls_G4_G04.fcs - a2010_O1T2pb05i_controls_G5_G05.fcs - a2010_O1T2pb05i_controls_G6_G06.fcs - a2010_O1T2pb05i_controls_G7_G07.fcs - a2010_O1T2pb05i_controls_G8_G08.fcs - a2010_O1T2pb05i_controls_G9_G09.fcs - a2010_O1T2pb05i_controls_H1_H01.fcs - a2010_O1T2pb05i_controls_H2_H02.fcs - a2010_O1T2pb05i_controls_H3_H03.fcs



Flow Sample/Specimen Details


a2006_O1T2pb05i_A1_A01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_A2_A02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_A3_A03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_A4_A04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_A5_A05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_A6_A06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_A7_A07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_A8_A08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_B1_B01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_B2_B02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_B3_B03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_B4_B04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_B5_B05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_B6_B06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_B7_B07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_B8_B08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_C1_C01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_C2_C02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_C3_C03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_C4_C04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_C5_C05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_C6_C06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_C7_C07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_C8_C08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_D1_D01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_D2_D02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_D3_D03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_D4_D04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_D5_D05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_D6_D06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_D7_D07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_D8_D08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_E1_E01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_E2_E02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_E3_E03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_E4_E04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_E5_E05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_E6_E06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_E7_E07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_E8_E08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_F1_F01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_F2_F02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_F3_F03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_F4_F04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2006_O1T2pb05i_controls_G10_G10.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_G11_G11.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_G12_G12.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_G1_G01.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_G2_G02.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_G3_G03.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_G4_G04.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_G5_G05.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_G6_G06.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_G7_G07.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_G8_G08.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_G9_G09.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_H1_H01.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_H2_H02.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2006_O1T2pb05i_controls_H3_H03.fcs :

Description control
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_A1_A01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_A2_A02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_A3_A03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_A4_A04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_A5_A05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_A6_A06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_A7_A07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_A8_A08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_B1_B01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_B2_B02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_B3_B03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_B4_B04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_B5_B05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_B6_B06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_B7_B07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_B8_B08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_C1_C01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_C2_C02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_C3_C03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_C4_C04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_C5_C05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_C6_C06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_C7_C07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_C8_C08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_D1_D01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_D2_D02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_D3_D03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_D4_D04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_D5_D05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_D6_D06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_D7_D07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_D8_D08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_E1_E01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_E2_E02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_E3_E03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_E4_E04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_E5_E05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_E6_E06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_E7_E07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_E8_E08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_F1_F01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_F2_F02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2007_O1T2pb05i_controls_G10_G10.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_G11_G11.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_G12_G12.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_G1_G01.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_G2_G02.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_G3_G03.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_G4_G04.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_G5_G05.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_G6_G06.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_G7_G07.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_G8_G08.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_G9_G09.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_H1_H01.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_H2_H02.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2007_O1T2pb05i_controls_H3_H03.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_A1_A01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_A2_A02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_A3_A03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_A4_A04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_A5_A05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_A6_A06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_A7_A07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_A8_A08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_B1_B01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_B2_B02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_B3_B03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_B4_B04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_B5_B05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_B6_B06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_B7_B07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_B8_B08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_C1_C01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_C2_C02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_C3_C03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_C4_C04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_C5_C05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_C6_C06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_C7_C07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_C8_C08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_D1_D01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_D2_D02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_D3_D03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_D4_D04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_D5_D05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_D6_D06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_D7_D07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_D8_D08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_E1_E01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_E2_E02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_E3_E03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_E4_E04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_E5_E05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_E6_E06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_E7_E07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_E8_E08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_F1_F01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_F2_F02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2008_O1T2pb05i_controls_G10_G10.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_G11_G11.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_G12_G12.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_G1_G01.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_G2_G02.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_G3_G03.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_G4_G04.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_G5_G05.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_G6_G06.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_G7_G07.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_G8_G08.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_G9_G09.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_H1_H01.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_H2_H02.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2008_O1T2pb05i_controls_H3_H03.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_A1_A01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_A2_A02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_A3_A03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_A4_A04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_A5_A05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_A6_A06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_A7_A07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_A8_A08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_B1_B01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_B2_B02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_B3_B03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_B4_B04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_B5_B05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_B6_B06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_B7_B07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_B8_B08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_C1_C01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_C2_C02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_C3_C03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_C4_C04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_C5_C05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_C6_C06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_C7_C07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_C8_C08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_D1_D01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_D2_D02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_D3_D03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_D4_D04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_D5_D05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_D6_D06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_D7_D07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_D8_D08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_E1_E01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_E2_E02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_E3_E03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_E4_E04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_E5_E05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_E6_E06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_E7_E07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_E8_E08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_F1_F01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_F2_F02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2009_O1T2pb05i_controls_G10_G10.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_G11_G11.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_G12_G12.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_G1_G01.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_G2_G02.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_G3_G03.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_G4_G04.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_G5_G05.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_G6_G06.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_G7_G07.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_G8_G08.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_G9_G09.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_H1_H01.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_H2_H02.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2009_O1T2pb05i_controls_H3_H03.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_A1_A01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_A2_A02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_A3_A03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_A4_A04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_A8_A08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_B1_B01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_B2_B02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_B3_B03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_B4_B04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_B5_B05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_B6_B06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_B7_B07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_B8_B08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_C1_C01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_C2_C02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_C3_C03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_C4_C04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_C5_C05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_C6_C06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_C7_C07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_C8_C08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_D1_D01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_D2_D02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_D3_D03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_D4_D04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_D5_D05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_D6_D06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_D7_D07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_D8_D08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_E1_E01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_E2_E02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_E3_E03.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_E4_E04.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_E5_E05.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_E6_E06.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_E7_E07.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_E8_E08.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_F1_F01.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_F2_F02.fcs :

Description Blood was drawn via sterile venipuncture into vacutainers containing 143 USP units of sodium-heparin (Becton Dickinson (BD); catalog no. 8019839). Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation as described in Jansen et al., J Immunol Methods 2008; 336(2): 183-192.
Sample source: [ biological ] Human peripheral blood - Homo sapiens - 22 - years - unknown - healthy - None
Sample characteristic: Expected/analyzed types of cells: monocytes, myeloid dendritic cells, plasmacytoid dendritic cells, B lymphocytes. T lymphocytes will also be present but not analyzed. Red blood cells are not present as they are lysed when samples are frozen in FACSLyse solution.
Sample treatment: see attached file 'plate map'
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
Cell surface protein CD11c anti-CD11c APC 5HCL3 BD Biosciences BD#340714
Intracellular Protein TNFa anti-TNFa Alexa 700 Mab11 BD Biosciences BD#557996
Cell Surface Protein CD14 anti-CD14 PE-Cy7 M5E2 eBioscience eBio#25-0149
Cell Surface Protein MHCII anti-MHCII PerCPCy5.5 TU36 BD Biosciences BD#custom
Intracellular Protein IFNa anti-IFNa FITC/OG A11 Antigenix Antigenix#MC100133
Intracellular Protein IL6 anti-IL6 APC-Cy7 MQ2-13A5 BD Biosciences BD#custom
Cell Surface Protein CD123 anti-CD123 AmCyan 9F5 BD Biosciences BD#custom
Intracellular Protein IL12p40/70 anti-IL12p40/70 Pacific Blue eBio: C8.6 eBioscience eBio#577129



a2010_O1T2pb05i_controls_G10_G10.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_G11_G11.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_G12_G12.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_G1_G01.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_G2_G02.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_G3_G03.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_G4_G04.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_G5_G05.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_G6_G06.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_G7_G07.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_G8_G08.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_G9_G09.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_H1_H01.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_H2_H02.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a



a2010_O1T2pb05i_controls_H3_H03.fcs :

Description controls
Sample source:
Sample characteristic: n/a
Sample treatment: n/a




Flow Cytometer Details

BD LSR II, Becton Dickinson (BD Biosciences) used for all 284 FCS files.

a2006_O1T2pb05i_A1_A01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_A2_A02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_A3_A03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_A4_A04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_A5_A05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_A6_A06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_A7_A07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_A8_A08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_B1_B01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_B2_B02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_B3_B03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_B4_B04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_B5_B05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_B6_B06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_B7_B07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_B8_B08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_C1_C01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_C2_C02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_C3_C03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_C4_C04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_C5_C05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_C6_C06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_C7_C07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_C8_C08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_D1_D01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_D2_D02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_D3_D03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_D4_D04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_D5_D05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_D6_D06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_D7_D07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_D8_D08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_E1_E01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_E2_E02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_E3_E03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_E4_E04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_E5_E05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_E6_E06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_E7_E07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_E8_E08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_F1_F01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_F2_F02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_F3_F03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_F4_F04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G10_G10.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G11_G11.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G12_G12.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G1_G01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G2_G02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G3_G03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G4_G04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G5_G05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G6_G06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G7_G07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G8_G08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_G9_G09.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_H1_H01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_H2_H02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2006_O1T2pb05i_controls_H3_H03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_A1_A01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_A2_A02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_A3_A03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_A4_A04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_A5_A05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_A6_A06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_A7_A07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_A8_A08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_B1_B01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_B2_B02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_B3_B03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_B4_B04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_B5_B05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_B6_B06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_B7_B07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_B8_B08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_C1_C01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_C2_C02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_C3_C03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_C4_C04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_C5_C05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_C6_C06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_C7_C07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_C8_C08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_D1_D01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_D2_D02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_D3_D03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_D4_D04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_D5_D05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_D6_D06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_D7_D07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_D8_D08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_E1_E01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_E2_E02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_E3_E03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_E4_E04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_E5_E05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_E6_E06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_E7_E07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_E8_E08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_F1_F01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_F2_F02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G10_G10.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G11_G11.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G12_G12.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G1_G01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G2_G02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G3_G03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G4_G04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G5_G05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G6_G06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G7_G07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G8_G08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_G9_G09.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_H1_H01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_H2_H02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2007_O1T2pb05i_controls_H3_H03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_A1_A01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_A2_A02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_A3_A03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_A4_A04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_A5_A05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_A6_A06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_A7_A07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_A8_A08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_B1_B01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_B2_B02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_B3_B03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_B4_B04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_B5_B05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_B6_B06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_B7_B07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_B8_B08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_C1_C01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_C2_C02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_C3_C03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_C4_C04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_C5_C05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_C6_C06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_C7_C07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_C8_C08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_D1_D01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_D2_D02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_D3_D03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_D4_D04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_D5_D05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_D6_D06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_D7_D07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_D8_D08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_E1_E01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_E2_E02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_E3_E03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_E4_E04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_E5_E05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_E6_E06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_E7_E07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_E8_E08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_F1_F01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_F2_F02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G10_G10.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G11_G11.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G12_G12.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G1_G01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G2_G02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G3_G03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G4_G04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G5_G05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G6_G06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G7_G07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G8_G08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_G9_G09.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_H1_H01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_H2_H02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2008_O1T2pb05i_controls_H3_H03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_A1_A01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_A2_A02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_A3_A03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_A4_A04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_A5_A05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_A6_A06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_A7_A07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_A8_A08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_B1_B01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_B2_B02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_B3_B03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_B4_B04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_B5_B05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_B6_B06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_B7_B07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_B8_B08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_C1_C01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_C2_C02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_C3_C03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_C4_C04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_C5_C05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_C6_C06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_C7_C07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_C8_C08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_D1_D01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_D2_D02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_D3_D03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_D4_D04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_D5_D05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_D6_D06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_D7_D07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_D8_D08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_E1_E01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_E2_E02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_E3_E03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_E4_E04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_E5_E05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_E6_E06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_E7_E07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_E8_E08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_F1_F01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_F2_F02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G10_G10.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G11_G11.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G12_G12.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G1_G01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G2_G02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G3_G03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G4_G04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G5_G05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G6_G06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G7_G07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G8_G08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_G9_G09.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_H1_H01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_H2_H02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2009_O1T2pb05i_controls_H3_H03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_A1_A01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_A2_A02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_A3_A03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_A4_A04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_A8_A08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_B1_B01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_B2_B02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_B3_B03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_B4_B04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_B5_B05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_B6_B06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_B7_B07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_B8_B08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_C1_C01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_C2_C02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_C3_C03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_C4_C04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_C5_C05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_C6_C06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_C7_C07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_C8_C08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_D1_D01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_D2_D02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_D3_D03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_D4_D04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_D5_D05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_D6_D06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_D7_D07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_D8_D08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_E1_E01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_E2_E02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_E3_E03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_E4_E04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_E5_E05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_E6_E06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_E7_E07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_E8_E08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_F1_F01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_F2_F02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G10_G10.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G11_G11.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G12_G12.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G1_G01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G2_G02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G3_G03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G4_G04.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G5_G05.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G6_G06.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G7_G07.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G8_G08.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_G9_G09.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_H1_H01.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_H2_H02.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

a2010_O1T2pb05i_controls_H3_H03.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates


Data Analysis Details

Figure cannot be displayed.