Experiment Overview

Repository ID: FR-FCM-ZZY7 Experiment name: Dataset 2-Flow Cytometer Sensitivity: A Quadratic Model MIFlowCyt score: 50.58%
Primary researcher: Faysal El Khettabi PI/manager: Ryan Brinkman Uploaded by: Faysal El Khettabi
Experiment dates: 2009-09-15 - 2009-09-15 Dataset uploaded: Fri Mar 23 12:36:38 -0400 2012 Last updated: Tue Jan 15 13:56:18 -0500 2013
Keywords: [flow cytometry] [High Throughput] [Doublet] [Mean Fluorescence Intensity (MFI)] [linear and quadratic regressions] [Q (detector efficiency) ] [B (background light level)] [Instrument Sensitivity] [K-means] [Molecules of Equivalent Soluble Fluorochrome (MESF)] [BD CS&T beads] [Kmeans Automatic Clustering] Pubmed IDs: None
Organizations: BC Cancer Agency, Terry Fox Lab, Vancouver, BC (Canada)
Purpose: Flow cytometer sensitivity has been well defined in the community and relates to two functions: how well a dimly fluorescent population is resolved from noise or an unstained population, and how well various dim staining populations can be distinguished from each other. In both cases the goal of finding optimal instrument settings to resolve populations based on their fluorescence can be achieved by examining bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. We used a data provided by University of Rochester Medical Center, Rochester NY. The experiment consisted of running BDtm CS&T beads on an LSRII cytometer at a series of voltages ranging from 200V to 900V at 10V intervals. This dataset was used to explore the effects of various perturbations to cytometer operations to the resulting estimates of Q and B values using the detector assigned to B515 that has the associated MESF-FITC values for the beads. MESF / ABC Information: FITC MESF-5 lot 9545 Peak_1=2562; Peak_2 =22018; Peak_3 =73297; Peak_4 =258460; Peak_5 =756128; MFIs are files with Duke-... MESFs are files with FITC MESF_...
Conclusion: We developed an automated approach to determine a cytometer’s detection efficiency (Q) and background illumination (B) independent of a pre-defined bead set, based on K-means clustering and quadratic regression. We showed that the quadratic formulation for modelling Q offers more physical insight about cytometry sensitivity than its truncated linear version. The developed quadratic regression approach that takes into account intrinsic variance from both the instrument and the bead product is very crucial in this experiment. An adequate illumination correction for the usefulness of the linear regression model is not possible because a number of voltages show a saturation in the bright peak with a CV equal to zero. In the traditional linear model this would incorrectly imply an illumination correction term of 0. As well, using K-means in place of manual gating provided for a fully automated method to calculate the Q and B quantities objectively and in a time-efficient manner. We validated our approach on this flow cytometry sensitivity data experiment through comparison to Q and B values obtained by manual analysis. Our results show a marked improvement in facilitating the use of automated analysis of flow cytometry sensitivity data sets and we conclude that using the quadratic model is more appropriate to help find optimal instrument settings that resolve populations based on their fluorescence. Using the K-means in place of manual gating provides for a fully automated method to calculate Q and B objectively and efficiently. Our approach is freely available through the R/Bioconductor package flowQB.
Comments: Data analysis was conducted using R/BioConductor. We used R’s implementation for K-means clustering to enable the automatic gating and the statistics extraction. The R functions for regression analysis lm was used to fit linear and quadratic regression models. The R function Poly was used to evaluate orthogonal polynomials involved in solving the resulting linear system of equations. We developed a freely available open source R/BioConductor package flowQB to automate the main steps for Q and B calculation, available through CRAN/Bioconductor. It includes a vignette that provides a task-oriented description of its functionality and an executable example that is intended to be used interactively. Thanks to everybody who provided FCS files for this collection! Please email Ryan Brinkman (rbrinkman@bccrc.ca) if you have any questions.
Quality control: FCS files have been tested in FlowJo and R flowCore package, which seem to read these without any issues and with a sufficient guarantee compliance with the FCS data file standard. MESF / ABC Information: FITC MESF-5 lot 9545 Peak_1=2562; Peak_2 =22018; Peak_3 =73297; Peak_4 =258460; Peak_5 =756128;


Flow Sample/Specimen Details


Duke_200.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage, the value of the voltage is in the name of the file, for instance, for Duke_250.fcs, the voltage is 250V and for Duke_610.fcs , the voltage is 610V, etc.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity
Staining: Individual reagents
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
CST bead particles - Proprietary FITC Unknown - CST bead particles
CST bead particles
Characteristic measured Analyte Analyte detector Analyte reporter Clone Manufacturer Catalog number Other
CST bead particles - Proprietary FITC Unknown - CST bead particles



Duke_210.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage, the value of the voltage is in the name of the file, for instance, for Duke_250.fcs, the voltage is 250V and for Duke_610.fcs , the voltage is 610V, etc.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_220.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage, the value of the voltage is in the name of the file, for instance, for Duke_250.fcs, the voltage is 250V and for Duke_610.fcs , the voltage is 610V, etc.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_230.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_240.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_250.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_260.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_270.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_280.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_290.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_300.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_310.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_320.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_330.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_340.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_350.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_360.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_370.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_380.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_390.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_400.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_410.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_420.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_430.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_440.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_450.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_460.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_470.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_480.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_490.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_500.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_510.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_520.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_530.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_540.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_550.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_560.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_570.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_580.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_590.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_600.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_610.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_620.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_630.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_640.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_650.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_660.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_670.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_680.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_690.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_700.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_710.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_720.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_730.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_740.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_750.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_760.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_770.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_780.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_790.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_800.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_810.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_820.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_830.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_840.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_850.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_860.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_870.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_880.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_890.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



Duke_900.fcs :

Description Examining CST bead particles (microspheres) that contain sub-populations with varying amounts of fluorescence intensity. These sub-population of beads are analyzed on a LSRII flow cytometer, the photomultiplier tubes (PMTs) record the fluorescence intensity signal for each given voltage.
Sample source: [ other ] CST BEADS
Sample characteristic: Identified seven distinct bead sub-populations
Sample treatment: CST beads (microspheres) that contain sub-populations with varying amounts of fluorescence intensity



FITC MESF_200.fcs :
No sample annotation exists for this FCS file.

FITC MESF_210.fcs :
No sample annotation exists for this FCS file.

FITC MESF_220.fcs :
No sample annotation exists for this FCS file.

FITC MESF_230.fcs :
No sample annotation exists for this FCS file.

FITC MESF_240.fcs :
No sample annotation exists for this FCS file.

FITC MESF_250.fcs :
No sample annotation exists for this FCS file.

FITC MESF_260.fcs :
No sample annotation exists for this FCS file.

FITC MESF_270.fcs :
No sample annotation exists for this FCS file.

FITC MESF_280.fcs :
No sample annotation exists for this FCS file.

FITC MESF_290.fcs :
No sample annotation exists for this FCS file.

FITC MESF_300.fcs :
No sample annotation exists for this FCS file.

FITC MESF_310.fcs :
No sample annotation exists for this FCS file.

FITC MESF_320.fcs :
No sample annotation exists for this FCS file.

FITC MESF_330.fcs :
No sample annotation exists for this FCS file.

FITC MESF_340.fcs :
No sample annotation exists for this FCS file.

FITC MESF_350.fcs :
No sample annotation exists for this FCS file.

FITC MESF_360.fcs :
No sample annotation exists for this FCS file.

FITC MESF_370.fcs :
No sample annotation exists for this FCS file.

FITC MESF_380.fcs :
No sample annotation exists for this FCS file.

FITC MESF_390.fcs :
No sample annotation exists for this FCS file.

FITC MESF_400.fcs :
No sample annotation exists for this FCS file.

FITC MESF_410.fcs :
No sample annotation exists for this FCS file.

FITC MESF_420.fcs :
No sample annotation exists for this FCS file.

FITC MESF_430.fcs :
No sample annotation exists for this FCS file.

FITC MESF_440.fcs :
No sample annotation exists for this FCS file.

FITC MESF_450.fcs :
No sample annotation exists for this FCS file.

FITC MESF_460.fcs :
No sample annotation exists for this FCS file.

FITC MESF_470.fcs :
No sample annotation exists for this FCS file.

FITC MESF_480.fcs :
No sample annotation exists for this FCS file.

FITC MESF_490.fcs :
No sample annotation exists for this FCS file.

FITC MESF_500.fcs :
No sample annotation exists for this FCS file.

FITC MESF_510.fcs :
No sample annotation exists for this FCS file.

FITC MESF_520.fcs :
No sample annotation exists for this FCS file.

FITC MESF_530.fcs :
No sample annotation exists for this FCS file.

FITC MESF_540.fcs :
No sample annotation exists for this FCS file.

FITC MESF_550.fcs :
No sample annotation exists for this FCS file.

FITC MESF_560.fcs :
No sample annotation exists for this FCS file.

FITC MESF_570.fcs :
No sample annotation exists for this FCS file.

FITC MESF_580.fcs :
No sample annotation exists for this FCS file.

FITC MESF_590.fcs :
No sample annotation exists for this FCS file.

FITC MESF_600.fcs :
No sample annotation exists for this FCS file.

FITC MESF_610.fcs :
No sample annotation exists for this FCS file.

FITC MESF_620.fcs :
No sample annotation exists for this FCS file.

FITC MESF_630.fcs :
No sample annotation exists for this FCS file.

FITC MESF_640.fcs :
No sample annotation exists for this FCS file.

FITC MESF_650.fcs :
No sample annotation exists for this FCS file.

FITC MESF_660.fcs :
No sample annotation exists for this FCS file.

FITC MESF_670.fcs :
No sample annotation exists for this FCS file.

FITC MESF_680.fcs :
No sample annotation exists for this FCS file.

FITC MESF_690.fcs :
No sample annotation exists for this FCS file.

FITC MESF_700.fcs :
No sample annotation exists for this FCS file.

FITC MESF_710.fcs :
No sample annotation exists for this FCS file.

FITC MESF_720.fcs :
No sample annotation exists for this FCS file.

FITC MESF_730.fcs :
No sample annotation exists for this FCS file.

FITC MESF_740.fcs :
No sample annotation exists for this FCS file.

FITC MESF_750.fcs :
No sample annotation exists for this FCS file.

FITC MESF_760.fcs :
No sample annotation exists for this FCS file.

FITC MESF_770.fcs :
No sample annotation exists for this FCS file.

FITC MESF_780.fcs :
No sample annotation exists for this FCS file.

FITC MESF_790.fcs :
No sample annotation exists for this FCS file.

FITC MESF_800.fcs :
No sample annotation exists for this FCS file.

FITC MESF_810.fcs :
No sample annotation exists for this FCS file.

FITC MESF_820.fcs :
No sample annotation exists for this FCS file.

FITC MESF_830.fcs :
No sample annotation exists for this FCS file.

FITC MESF_840.fcs :
No sample annotation exists for this FCS file.

FITC MESF_850.fcs :
No sample annotation exists for this FCS file.

FITC MESF_860.fcs :
No sample annotation exists for this FCS file.

FITC MESF_870.fcs :
No sample annotation exists for this FCS file.

FITC MESF_880.fcs :
No sample annotation exists for this FCS file.

FITC MESF_890.fcs :
No sample annotation exists for this FCS file.

FITC MESF_900.fcs :
No sample annotation exists for this FCS file.


Flow Cytometer Details


Duke_200.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_210.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_220.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_230.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_240.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_250.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_260.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_270.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_280.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_290.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_300.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_310.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_320.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_330.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_340.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_350.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_360.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_370.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_380.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_390.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_400.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_410.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_420.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_430.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_440.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_450.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_460.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_470.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_480.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_490.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_500.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_510.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_520.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_530.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_540.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_550.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_560.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_570.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_580.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_590.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_600.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_610.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_620.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_630.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_640.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_650.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_660.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_670.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_680.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_690.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_700.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_710.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_720.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_730.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_740.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_750.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_760.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_770.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_780.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_790.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_800.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_810.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_820.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_830.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_840.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_850.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_860.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_870.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_880.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_890.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

Duke_900.fcs
Manufacturer Becton Dickinson (BD Biosciences) Default instrument settings used.
Model BD LSR II
Optical filter installation dates

FITC MESF_200.fcs
No instrumentation information provided for this FCS file.
FITC MESF_210.fcs
No instrumentation information provided for this FCS file.
FITC MESF_220.fcs
No instrumentation information provided for this FCS file.
FITC MESF_230.fcs
No instrumentation information provided for this FCS file.
FITC MESF_240.fcs
No instrumentation information provided for this FCS file.
FITC MESF_250.fcs
No instrumentation information provided for this FCS file.
FITC MESF_260.fcs
No instrumentation information provided for this FCS file.
FITC MESF_270.fcs
No instrumentation information provided for this FCS file.
FITC MESF_280.fcs
No instrumentation information provided for this FCS file.
FITC MESF_290.fcs
No instrumentation information provided for this FCS file.
FITC MESF_300.fcs
No instrumentation information provided for this FCS file.
FITC MESF_310.fcs
No instrumentation information provided for this FCS file.
FITC MESF_320.fcs
No instrumentation information provided for this FCS file.
FITC MESF_330.fcs
No instrumentation information provided for this FCS file.
FITC MESF_340.fcs
No instrumentation information provided for this FCS file.
FITC MESF_350.fcs
No instrumentation information provided for this FCS file.
FITC MESF_360.fcs
No instrumentation information provided for this FCS file.
FITC MESF_370.fcs
No instrumentation information provided for this FCS file.
FITC MESF_380.fcs
No instrumentation information provided for this FCS file.
FITC MESF_390.fcs
No instrumentation information provided for this FCS file.
FITC MESF_400.fcs
No instrumentation information provided for this FCS file.
FITC MESF_410.fcs
No instrumentation information provided for this FCS file.
FITC MESF_420.fcs
No instrumentation information provided for this FCS file.
FITC MESF_430.fcs
No instrumentation information provided for this FCS file.
FITC MESF_440.fcs
No instrumentation information provided for this FCS file.
FITC MESF_450.fcs
No instrumentation information provided for this FCS file.
FITC MESF_460.fcs
No instrumentation information provided for this FCS file.
FITC MESF_470.fcs
No instrumentation information provided for this FCS file.
FITC MESF_480.fcs
No instrumentation information provided for this FCS file.
FITC MESF_490.fcs
No instrumentation information provided for this FCS file.
FITC MESF_500.fcs
No instrumentation information provided for this FCS file.
FITC MESF_510.fcs
No instrumentation information provided for this FCS file.
FITC MESF_520.fcs
No instrumentation information provided for this FCS file.
FITC MESF_530.fcs
No instrumentation information provided for this FCS file.
FITC MESF_540.fcs
No instrumentation information provided for this FCS file.
FITC MESF_550.fcs
No instrumentation information provided for this FCS file.
FITC MESF_560.fcs
No instrumentation information provided for this FCS file.
FITC MESF_570.fcs
No instrumentation information provided for this FCS file.
FITC MESF_580.fcs
No instrumentation information provided for this FCS file.
FITC MESF_590.fcs
No instrumentation information provided for this FCS file.
FITC MESF_600.fcs
No instrumentation information provided for this FCS file.
FITC MESF_610.fcs
No instrumentation information provided for this FCS file.
FITC MESF_620.fcs
No instrumentation information provided for this FCS file.
FITC MESF_630.fcs
No instrumentation information provided for this FCS file.
FITC MESF_640.fcs
No instrumentation information provided for this FCS file.
FITC MESF_650.fcs
No instrumentation information provided for this FCS file.
FITC MESF_660.fcs
No instrumentation information provided for this FCS file.
FITC MESF_670.fcs
No instrumentation information provided for this FCS file.
FITC MESF_680.fcs
No instrumentation information provided for this FCS file.
FITC MESF_690.fcs
No instrumentation information provided for this FCS file.
FITC MESF_700.fcs
No instrumentation information provided for this FCS file.
FITC MESF_710.fcs
No instrumentation information provided for this FCS file.
FITC MESF_720.fcs
No instrumentation information provided for this FCS file.
FITC MESF_730.fcs
No instrumentation information provided for this FCS file.
FITC MESF_740.fcs
No instrumentation information provided for this FCS file.
FITC MESF_750.fcs
No instrumentation information provided for this FCS file.
FITC MESF_760.fcs
No instrumentation information provided for this FCS file.
FITC MESF_770.fcs
No instrumentation information provided for this FCS file.
FITC MESF_780.fcs
No instrumentation information provided for this FCS file.
FITC MESF_790.fcs
No instrumentation information provided for this FCS file.
FITC MESF_800.fcs
No instrumentation information provided for this FCS file.
FITC MESF_810.fcs
No instrumentation information provided for this FCS file.
FITC MESF_820.fcs
No instrumentation information provided for this FCS file.
FITC MESF_830.fcs
No instrumentation information provided for this FCS file.
FITC MESF_840.fcs
No instrumentation information provided for this FCS file.
FITC MESF_850.fcs
No instrumentation information provided for this FCS file.
FITC MESF_860.fcs
No instrumentation information provided for this FCS file.
FITC MESF_870.fcs
No instrumentation information provided for this FCS file.
FITC MESF_880.fcs
No instrumentation information provided for this FCS file.
FITC MESF_890.fcs
No instrumentation information provided for this FCS file.
FITC MESF_900.fcs
No instrumentation information provided for this FCS file.


Data Analysis Details

No illustrations provided.