Experiment Overview

Repository ID: FR-FCM-ZZYR Experiment name: User-defined protein marker assay development for characterization of circulating tumor cells using the CellSearch system MIFlowCyt score: 29.25%
Primary researcher: Alison Allan PI/manager: Alison Allan Uploaded by: Alison Allan
Experiment dates: 2008-10-01 - 2011-08-31 Dataset uploaded: Tue Jul 10 15:05:29 -0400 2012 Last updated: Sat Jan 12 21:24:47 -0500 2013
Keywords: [Cancer] [metastasis] [circulating tumor cells (CTCs)] [CD44] [M-30] [CellSearch® system] [user-defined marker] [CTC characterization] Manuscripts: [22899576] Cytalogo
Organizations: London Regional Cancer Program, London Health Sciences Centre, Cancer Research, Flow Cytometry/Special Hematology, London, Ontario (Canada)
Purpose: The purpose of the flow cytometry experiments in this study was to assess expression/presence of circulating tumor cell (CTC)-associated markers/characteristics in immortalized human cancer cell lines and normal human blood (i.e. percentage of cell population expressing the marker/characteristic of interest).
Conclusion: Flow cytometry analysis is a useful way of validating the percentage of cells within a given cell population that are positive for CTC-associated markers/characteristics of interest. This analysis provides a valuable starting point for optimization of user-defined markers for CTC characterization using the CellSearch® system (Veridex)
Comments: None
Funding: Not disclosed
Quality control: For marker expression analysis, FMO or appropriate IgG isotype controls were used. For experiments involving assessment of apoptosis, untreated cells were used as a negative control.


Flow Sample/Specimen Details


468 Treated Cells only 00001003 493.LMD :
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468 Treated Cells only 00001012 502.LMD :
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468 Treated Cells only 00001067 557.LMD :
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468 Treated M30 + DAPI 00001006 496.LMD :
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468 Treated M30 + DAPI 00001070 560.LMD :
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468 Treated M30 DAPI 00001015 505.LMD :
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468 Untreated Cells only 00000999 489.LMD :
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468 Untreated Cells only 00001008 498.LMD :
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468 Untreated Cells only 00001063 553.LMD :
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468 Untreated M30 + DAPI 00001002 492.LMD :
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468 Untreated M30 + DAPI 00001066 556.LMD :
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468 Untreated M30 DAPI 00001011 501.LMD :
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Flow Cytometer Details

None provided


Data Analysis Details

No illustrations provided.