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Experiment Overview

Repository ID: FR-FCM-ZZ3Y Experiment name: OMIP-016: Characterization of Antigen-Responsive macaque and human T-cells (Online Fig 7&8) MIFlowCyt score: 84.87%
Primary researcher: Antonio Cosma PI/manager: Antonio Cosma Uploaded by: Antonio Cosma
Experiment dates: 2012-08-06 - 2012-08-10 Dataset uploaded: Sep 2012 Last updated: Mar 2013
Keywords: [Innate Immune Response] [Intracellular cytokines; CD8 T cells] [CD4 T cells; HIV; SIV; vaccines] Manuscripts: [23184609] Cytalogo
Organizations: CEA, Division of Immuno-Virology, DSV/iMETI, Fontenay-aux-Roses, (France)
Purpose: Evaluate the freeze and thaw procedure
Conclusion: The freeze and thaw procedure can be used as breaking point in the ICS panel described in the OMIP
Comments: None
Funding: Not disclosed
Quality control: The freeze and thaw procedure has been compared to the classical procedure without breaking point. Non stimulated and SEB stimulated samples were used


Experiment variables

Conditions
· NS 20792_20110303_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · 20792_20110303_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · CTRLS AND BEADS_UNSTAINED_09082012.fcs · DONNEUR 2_20091211_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · DONNEUR 2_20091211_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs
· SEB 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 40 SEC HOT BATH_001.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH_001.fcs

Individuals
· 20792 20792_20110303_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · 20792_20110303_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 40 SEC HOT BATH_001.fcs
· DONNEUR 2 DONNEUR 2_20091211_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · DONNEUR 2_20091211_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH_001.fcs

Timepoints
· No Break 20792_20110303_NS_WITHOUT FREEZING ON DAY 2.fcs · 20792_20110303_SEB_WITHOUT FREEZING ON DAY 2.fcs · DONNEUR 2_20091211_NS_WITHOUT FREEZING ON DAY 2.fcs · DONNEUR 2_20091211_SEB_WITHOUT FREEZING ON DAY 2.fcs
· Break (30' Thaw) 20792_20110303_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · DONNEUR 2_20091211_NS_FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 30MIN INCUBATOR.fcs
· Break (40'' Thaw) 20792_20110303_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · 20792_20110303_SEB_ FREEZING ON DAY 2+THAWING 40 SEC HOT BATH_001.fcs · DONNEUR 2_20091211_NS_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH.fcs · DONNEUR 2_20091211_SEB_ FREEZING ON DAY 2+THAWING 40 SEC WATER BATH_001.fcs

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