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Experiment Overview

Repository ID: FR-FCM-Z2EP Experiment name: HSC soluble factor MIFlowCyt score: 97.20%
Primary researcher: Aidan Gilchrist PI/manager: Brendan Harley Uploaded by: Aidan Gilchrist
Experiment dates: 2019-09-13 - 2019-11-14 Dataset uploaded: Jan 2020 Last updated: Jan 2020
Keywords: [hematopoietic stem cells] [quiescence] [Mesenchymal stem cells] [artificial stem cell niche] Manuscripts:
Organizations: University of Illinois at Urbana-Champaign, Flow Cytometry Facility Roy J. Carver Biotechnology Center, Urbana, Illinois (United States)
Purpose: Immunophenotyping of hematopoietic lineage cells that were culture in GelMA hydrogels with and without mesenchymal stromal cells. Cultures were exogenously stimulated with cytokines identified with an interative partial least squares regression filter method.
Conclusion: A minimum cocktail of cytokines (TGFβ-1, MMP-3, TROY, and c-RP) identified by a PLSR model was shown to increase the progenitor potential of the culture system
Comments: None
Funding: National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health under Award Numbers R01 DK099528 (B.A.C.H) and F31 DK117514 (A.E.G.), as well as by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under Award Numbers R21 EB018481 (B.A.C.H.) and T32 EB019944 (A.E.G.)
Quality control: Fluorescence minus one controls were used with each flow experiment. All conditions were performed with a minimum of n=3.


Experiment variables

Conditions
· 1:0 TGFβ-1 20190913_11_001.fcs · 20190913_12_002.fcs · 20190913_13_003.fcs · 20190913_14_004.fcs · 20190927_11_001.fcs · 20190927_12_002.fcs · 20190927_13_003.fcs · 20190927_14_004.fcs · 20191114_11_012.fcs · 20191114_1_011.fcs
· 1:0 MMP-3 active 20191114_72_024.fcs · 20191114_73_025.fcs · 20191114_74_026.fcs · 20191114_75_027.fcs · 20191114_76_028.fcs · 20191114_7_023.fcs
· 1:0 MMP-3 latent 20190913_21_005.fcs · 20190913_22_006.fcs · 20190913_23_007.fcs · 20190913_24_008.fcs · 20190927_21_005.fcs · 20190927_22_dropped_006.fcs · 20190927_23_007.fcs · 20190927_24_008.fcs
· 1:0 TROY 20190913_31_009.fcs · 20190913_32_010.fcs · 20190913_33_011.fcs · 20190913_34_012.fcs · 20190927_31_009.fcs · 20190927_32_010.fcs · 20190927_33_011.fcs · 20190927_34_012.fcs
· 1:0 c-RP 20190913_41_013.fcs · 20190913_42_014.fcs · 20190913_43_015.fcs · 20190913_44_016.fcs · 20190927_41_013.fcs · 20190927_42_014.fcs · 20190927_43_015.fcs · 20190927_44_016.fcs
· 1:0 Combo active 20191114_82_030.fcs · 20191114_83_031.fcs · 20191114_84_032.fcs · 20191114_85_033.fcs · 20191114_86_034.fcs · 20191114_8_029.fcs
· 1:0 Combo latent 20190913_51_017.fcs · 20190913_52_018.fcs · 20190913_53_019.fcs · 20190913_54_020.fcs · 20190927_51_017.fcs · 20190927_52_018.fcs · 20190927_53_019.fcs · 20190927_54_020.fcs · 20191114_22_014.fcs · 20191114_2_013.fcs
· 1:1 TGFβ-1 20190927_71_025.fcs · 20190927_72_026.fcs · 20190927_73_027.fcs · 20190927_74_028.fcs · 20191114_44_018.fcs · 20191114_4_017.fcs
· 1:1 MMP-3 active 20191114_102_042.fcs · 20191114_103_043.fcs · 20191114_104_044.fcs · 20191114_105_045.fcs · 20191114_106_046.fcs · 20191114_10_041.fcs
· 1:1 MMP-3 latent 20190927_81_029.fcs · 20190927_82_030.fcs · 20190927_83_031.fcs · 20190927_84_032.fcs
· 1:1 TROY 20190927_91_033.fcs · 20190927_92_034.fcs · 20190927_93_035.fcs · 20190927_94_036.fcs
· 1:1 c-RP 20190927_101_037.fcs · 20190927_102_038.fcs · 20190927_103_039.fcs · 20190927_104_040.fcs
· 1:1 Combo active 20191114_111_047.fcs · 20191114_112_048.fcs · 20191114_113_049.fcs · 20191114_114_050.fcs · 20191114_115_051.fcs · 20191114_116_052.fcs
· 1:1 Combo latent 20190927_111_041.fcs · 20190927_112_042.fcs · 20190927_113_043.fcs · 20190927_114_044.fcs · 20191114_55_020.fcs · 20191114_5_019.fcs
· 1:0 None 20190913_61_021.fcs · 20190913_62_022.fcs · 20190913_63_023.fcs · 20190913_64_024.fcs · 20190927_61_021.fcs · 20190927_62_022.fcs · 20190927_63_023.fcs · 20190927_64_024.fcs · 20191114_33_016.fcs · 20191114_3_015.fcs · 20191114_92_036.fcs · 20191114_93_037.fcs · 20191114_94_038.fcs · 20191114_95_039.fcs · 20191114_96_040.fcs · 20191114_9_035.fcs
· 1:1 None 20190913_71_025.fcs · 20190913_72_026.fcs · 20190913_73_027.fcs · 20190913_74_028.fcs · 20190927_121_045.fcs · 20190927_122_046.fcs · 20190927_123_047.fcs · 20190927_124_048.fcs · 20191114_122_054.fcs · 20191114_123_055.fcs · 20191114_124_056.fcs · 20191114_125_057.fcs · 20191114_126_058.fcs · 20191114_12_053.fcs · 20191114_66_022.fcs · 20191114_6_021.fcs
· flow rate study 20190913_flow rate_2000_041.fcs · 20190913_flow rate_4000_042.fcs · 20190913_practice_001_039.fcs · 20190913_practice_002_040.fcs · 20190913_practice_038.fcs

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