Experiment Overview
| Repository ID: | FR-FCM-Z2TM | Experiment name: | In vitro T cell expansion | MIFlowCyt score: | 70.00% |
| Primary researcher: | George Elias | PI/manager: | George Elias | Uploaded by: | George Elias |
| Experiment dates: | 2019-05-01 - 2019-07-31 | Dataset uploaded: | Aug 2020 | Last updated: | Aug 2020 |
| Keywords: | [CFSE] [CD4 T cells] [In vitro expansion] | Manuscripts: | [35074048] |
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| Organizations: |
Antwerp University, Laboratory of Experimental Hematology, Antwerp, Antwerp (Belgium)
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| Purpose: | Identification of vaccine-specific TCR-beta clonotypes by sequencing to track those clonotypes in memory CD4 T cell repertoires at day 0 pre-vaccination and day 60 post-vaccination with Engerix-B vaccine. | ||||
| Conclusion: | We detected a significant rise in the frequency of unique vaccine-specific TCRβ sequences within memory CD4 T cell repertoire at day 60 post-vaccination compared to pre-vaccination. | ||||
| Comments: | Peripheral blood mononuclear cells from day 60 were labeled with carboxyfluorescein succinimidyl ester (CFSE) and stimulated with a pool of peptides spanning hepatitis B (HB) surface antigen (HBsAg) and selected single peptides (from epitope mapping). At day 7 of in vitro expansion, we sorted CFSElow CD4 T cells and extracted mRNA for quantitative assessment of vaccine-specific TCR? clonotypes by sequencing. | ||||
| Funding: | 1. University of Antwerp [BOF Concerted Research Action (PS ID 30730) Methusalem funding; Industrial Research Fund SBO]. 2. Research Foundation Flanders (1861219N grant to B. Ogunjimi and FWO SB grant to N. De Neuter. | ||||
| Quality control: | Gates for CFSElow CD4 T cells were set using cells left unstimulated. | ||||
Experiment variables
| Conditions | |
|---|---|
| · NC | H02_NC.fcs · H03_NC.fcs · H04_NC.fcs · H04_NC.fcs-(1) · H06_NC.fcs · H06_NC.fcs-(1) · H07_NC.fcs · H08_NC.fcs · H10_NC.fcs · H11_NC.fcs · H13_NC.fcs · H14_NC.fcs · H14_NC.fcs-(1) · H17_NC.fcs · H17_NC.fcs-(1) · H18_NC.fcs · H19_NC.fcs · H20_NC.fcs · H21_NC.fcs · H21_NC.fcs-(1) · H22_NC.fcs · H23_E_NC.fcs · H23_NC.fcs · H23_NC.fcs-(1) · H24_NC.fcs · H24_NC.fcs-(1) · H25_NC.fcs · H25_NC.fcs-(1) · H26_NC.fcs · H26_NC.fcs-(1) · H28_NC.fcs · H29_NC.fcs · H30_NC.fcs · H30_NC.fcs-(1) · H31_NC.fcs · H32_NC.fcs · H33_NC.fcs · H34_NC.fcs · H35_NC.fcs · H36_NC.fcs · H38_NC.fcs · H38_NC.fcs-(1) · H39_NC.fcs · H39_NC.fcs-(1) · H40_NC.fcs · H41_NC.fcs · H42_NC.fcs |
| · PP | H02_PP.fcs · H03_PP.fcs · H04_PP.fcs · H04_PP.fcs-(1) · H06_PP.fcs · H06_PP.fcs-(1) · H07_PP.fcs · H08_PP.fcs · H10_PP.fcs · H11_PP.fcs · H13_PP.fcs · H14_PP.fcs · H14_PP.fcs-(1) · H17_PP.fcs · H17_PP.fcs-(1) · H18_PP.fcs · H19_PP.fcs · H20_PP.fcs · H21_PP.fcs · H21_PP.fcs-(1) · H22_PP.fcs · H23_E_PP.fcs · H23_PP.fcs · H23_PP.fcs-(1) · H24_PP.fcs · H24_PP.fcs-(1) · H25_PP.fcs · H25_PP.fcs-(1) · H26_PP.fcs · H26_PP.fcs-(1) · H28_PP.fcs · H29_PP.fcs · H30_PP.fcs · H30_PP.fcs-(1) · H31_PP.fcs · H32_PP.fcs · H33_PP.fcs · H34_PP.fcs · H35_PP.fcs · H36_PP.fcs · H38_PP.fcs · H38_PP.fcs-(1) · H39_PP.fcs · H39_PP.fcs-(1) · H40_PP.fcs · H41_PP.fcs · H42_PP.fcs |
| · P | H02_P34.fcs · H02_P35.fcs · H02_P38.fcs · H02_P50.fcs · H02_P51.fcs · H02_P54.fcs · H04_P04.fcs · H04_P05.fcs · H04_P20.fcs · H04_P21.fcs · H04_P52.fcs · H04_P53.fcs · H06_P05.fcs · H06_P07.fcs · H06_P21.fcs · H06_P23.fcs · H06_P37.fcs · H06_P39.fcs · H07_P17.fcs · H07_P33.fcs · H08_P41.fcs · H08_P42.fcs · H08_P47.fcs · H10_P1.fcs · H10_P33.fcs · H10_P41.fcs · H10_P49.fcs · H10_P9.fcs · H13_P16.fcs · H13_P24.fcs · H13_P8.fcs · H14_P45.fcs · H14_P47.fcs · H17_P09.fcs · H17_P10.fcs · H17_P14.fcs · H17_P41.fcs · H17_P42.fcs · H17_P46.fcs · H18_P37.fcs · H18_P38.fcs · H18_P5.fcs · H18_P6.fcs · H19_P16.fcs · H19_P40.fcs · H19_P8.fcs · H20_P1.fcs · H20_P6.fcs · H21_P01.fcs · H21_P02.fcs · H21_P03.fcs · H21_P41.fcs · H21_P42.fcs · H21_P43.fcs · H21_P52.fcs · H22_P10.fcs · H22_P12.fcs · H22_P13.fcs · H22_P16.fcs · H23_E_P43.fcs · H23_E_P46.fcs · H23_E_P47.fcs · H23_E_P48.fcs · H23_E_P51.fcs · H23_E_P54.fcs · H24_P18.fcs · H24_P20.fcs · H24_P22.fcs · H24_P23.fcs · H24_P24.fcs · H24_P46.fcs · H26_P07.fcs · H26_P17.fcs · H26_P23.fcs · H26_P41.fcs · H26_P47.fcs · H26_P49.fcs · H28_P36.fcs · H28_P38.fcs · H28_P39.fcs · H28_P40.fcs · H28_P52.fcs · H28_P54.fcs · H29_P1.fcs · H29_P23.fcs · H29_P46.fcs · H29_P47.fcs · H29_P6.fcs · H29_P7.fcs · H30_P19.fcs · H30_P20.fcs · H30_P22.fcs · H30_P43.fcs · H30_P46.fcs · H30_P54.fcs · H31_P33.fcs · H31_P37.fcs · H31_P38.fcs · H31_P49.fcs · H31_P53.fcs · H31_P54.fcs · H32_P1.fcs · H32_P28.fcs · H32_P31.fcs · H32_P4.fcs · H32_P6.fcs · H32_P7.fcs · H33_P1.fcs · H33_P41.fcs · H33_P45.fcs · H33_P47.fcs · H33_P5.fcs · H33_P7.fcs · H34_P2.fcs · H34_P4.fcs · H34_P5.fcs · H34_P6.fcs · H34_P7.fcs · H35_P21.fcs · H35_P22.fcs · H35_P23.fcs · H35_P45.fcs · H35_P46.fcs · H35_P47.fcs · H36_P38.fcs · H36_P39.fcs · H36_P46.fcs · H36_P47.fcs · H36_P48.fcs · H36_P54.fcs · H38_P02.fcs · H38_P05.fcs · H38_P33.fcs · H38_P34.fcs · H38_P35.fcs · H38_P37.fcs · H39_P34.fcs · H39_P35.fcs · H39_P49.fcs · H39_P50.fcs · H39_P51.fcs · H39_P53.fcs · H40_P33.fcs · H40_P37.fcs · H40_P38.fcs · H40_P39.fcs · H40_P40.fcs · H42_P1.fcs · H42_P25.fcs · H42_P33.fcs · H42_P41.fcs |
| Individuals | |
|---|---|
| · H02 | H02_NC.fcs · H02_P34.fcs · H02_P35.fcs · H02_P38.fcs · H02_P50.fcs · H02_P51.fcs · H02_P54.fcs · H02_PP.fcs |
| · H03 | H03_NC.fcs · H03_PP.fcs |
| · H04 | H04_NC.fcs · H04_NC.fcs-(1) · H04_P04.fcs · H04_P05.fcs · H04_P20.fcs · H04_P21.fcs · H04_P52.fcs · H04_P53.fcs · H04_PP.fcs · H04_PP.fcs-(1) |
| · H06 | H06_NC.fcs · H06_NC.fcs-(1) · H06_P05.fcs · H06_P07.fcs · H06_P21.fcs · H06_P23.fcs · H06_P37.fcs · H06_P39.fcs · H06_PP.fcs · H06_PP.fcs-(1) |
| · H07 | H07_NC.fcs · H07_P17.fcs · H07_P33.fcs · H07_PP.fcs |
| · H08 | H08_NC.fcs · H08_P41.fcs · H08_P42.fcs · H08_P47.fcs · H08_PP.fcs |
| · H10 | H10_NC.fcs · H10_P1.fcs · H10_P33.fcs · H10_P41.fcs · H10_P49.fcs · H10_P9.fcs · H10_PP.fcs |
| · H11 | H11_NC.fcs · H11_PP.fcs |
| · H13 | H13_NC.fcs · H13_P16.fcs · H13_P24.fcs · H13_P8.fcs · H13_PP.fcs |
| · H14 | H14_NC.fcs · H14_NC.fcs-(1) · H14_P45.fcs · H14_P47.fcs · H14_PP.fcs · H14_PP.fcs-(1) |
| · H17 | H17_NC.fcs · H17_NC.fcs-(1) · H17_P09.fcs · H17_P10.fcs · H17_P14.fcs · H17_P41.fcs · H17_P42.fcs · H17_P46.fcs · H17_PP.fcs · H17_PP.fcs-(1) |
| · H18 | H18_NC.fcs · H18_P37.fcs · H18_P38.fcs · H18_P5.fcs · H18_P6.fcs · H18_PP.fcs |
| · H19 | H19_NC.fcs · H19_P16.fcs · H19_P40.fcs · H19_P8.fcs · H19_PP.fcs |
| · H20 | H20_NC.fcs · H20_P1.fcs · H20_P6.fcs · H20_PP.fcs |
| · H21 | H21_NC.fcs · H21_NC.fcs-(1) · H21_P01.fcs · H21_P02.fcs · H21_P03.fcs · H21_P41.fcs · H21_P42.fcs · H21_P43.fcs · H21_P52.fcs · H21_PP.fcs · H21_PP.fcs-(1) |
| · H22 | H22_NC.fcs · H22_P10.fcs · H22_P12.fcs · H22_P13.fcs · H22_P16.fcs · H22_PP.fcs |
| · H23 | H23_E_NC.fcs · H23_E_P43.fcs · H23_E_P46.fcs · H23_E_P47.fcs · H23_E_P48.fcs · H23_E_P51.fcs · H23_E_P54.fcs · H23_E_PP.fcs · H23_NC.fcs · H23_NC.fcs-(1) · H23_PP.fcs · H23_PP.fcs-(1) |
| · H24 | H24_NC.fcs · H24_NC.fcs-(1) · H24_P18.fcs · H24_P20.fcs · H24_P22.fcs · H24_P23.fcs · H24_P24.fcs · H24_P46.fcs · H24_PP.fcs · H24_PP.fcs-(1) |
| · H25 | H25_NC.fcs · H25_NC.fcs-(1) · H25_PP.fcs · H25_PP.fcs-(1) |
| · H26 | H26_NC.fcs · H26_NC.fcs-(1) · H26_P07.fcs · H26_P17.fcs · H26_P23.fcs · H26_P41.fcs · H26_P47.fcs · H26_P49.fcs · H26_PP.fcs · H26_PP.fcs-(1) |
| · H28 | H28_NC.fcs · H28_P36.fcs · H28_P38.fcs · H28_P39.fcs · H28_P40.fcs · H28_P52.fcs · H28_P54.fcs · H28_PP.fcs |
| · H29 | H29_NC.fcs · H29_P1.fcs · H29_P23.fcs · H29_P46.fcs · H29_P47.fcs · H29_P6.fcs · H29_P7.fcs · H29_PP.fcs |
| · H30 | H30_NC.fcs · H30_NC.fcs-(1) · H30_P19.fcs · H30_P20.fcs · H30_P22.fcs · H30_P43.fcs · H30_P46.fcs · H30_P54.fcs · H30_PP.fcs · H30_PP.fcs-(1) |
| · H31 | H31_NC.fcs · H31_P33.fcs · H31_P37.fcs · H31_P38.fcs · H31_P49.fcs · H31_P53.fcs · H31_P54.fcs · H31_PP.fcs |
| · H32 | H32_NC.fcs · H32_P1.fcs · H32_P28.fcs · H32_P31.fcs · H32_P4.fcs · H32_P6.fcs · H32_P7.fcs · H32_PP.fcs |
| · H33 | H33_NC.fcs · H33_P1.fcs · H33_P41.fcs · H33_P45.fcs · H33_P47.fcs · H33_P5.fcs · H33_P7.fcs · H33_PP.fcs |
| · H34 | H34_NC.fcs · H34_P2.fcs · H34_P4.fcs · H34_P5.fcs · H34_P6.fcs · H34_P7.fcs · H34_PP.fcs |
| · H35 | H35_NC.fcs · H35_P21.fcs · H35_P22.fcs · H35_P23.fcs · H35_P45.fcs · H35_P46.fcs · H35_P47.fcs · H35_PP.fcs |
| · H36 | H36_NC.fcs · H36_P38.fcs · H36_P39.fcs · H36_P46.fcs · H36_P47.fcs · H36_P48.fcs · H36_P54.fcs · H36_PP.fcs |
| · H38 | H38_NC.fcs · H38_NC.fcs-(1) · H38_P02.fcs · H38_P05.fcs · H38_P33.fcs · H38_P34.fcs · H38_P35.fcs · H38_P37.fcs · H38_PP.fcs · H38_PP.fcs-(1) |
| · H39 | H39_NC.fcs · H39_NC.fcs-(1) · H39_P34.fcs · H39_P35.fcs · H39_P49.fcs · H39_P50.fcs · H39_P51.fcs · H39_P53.fcs · H39_PP.fcs · H39_PP.fcs-(1) |
| · H40 | H40_NC.fcs · H40_P33.fcs · H40_P37.fcs · H40_P38.fcs · H40_P39.fcs · H40_P40.fcs · H40_PP.fcs |
| · H41 | H41_NC.fcs · H41_PP.fcs |
| · H42 | H42_NC.fcs · H42_P1.fcs · H42_P25.fcs · H42_P33.fcs · H42_P41.fcs · H42_PP.fcs |
