Experiment Overview
| Repository ID: | FR-FCM-Z2TN | Experiment name: | Epitope mapping | MIFlowCyt score: | 56.00% |
| Primary researcher: | George Elias | PI/manager: | George Elias | Uploaded by: | George Elias |
| Experiment dates: | 2018-09-03 - 2018-12-21 | Dataset uploaded: | Aug 2020 | Last updated: | Aug 2020 |
| Keywords: | [CD40L] [epitope mapping] [HBsAg epitopes] | Manuscripts: | [35074048] |
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| Organizations: |
Antwerp University, Laboratory of Experimental Hematology, Antwerp, Antwerp (Belgium)
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| Purpose: | Epitope mapping of the CD4 T cell response to Engerix-B vaccine | ||||
| Conclusion: | After deconvolution of the response to matrix peptide pools, single peptides against which a CD4 T cell response was detected were selected for in vitro expansion and sorting of single peptide-specific CD4 T cells for TCR-beta sequencing. Aim was to track vaccine-specific CD4 T clonotypes within memory CD4 T cell repertoire pre and post-vaccination. | ||||
| Comments: | A set of 15-mers peptides with an 11-amino acid overlap spanning the 226 amino acids along the small S protein of hepatitis B (HB) surface antigen (HBsAg), The set, composed of 54 single peptides was used in a matrix-based strategy to map epitopes against which the immune response is directed. The matrix layout enables efficient identification of epitopes within the antigen using a minimal number of cells. For this purpose, a matrix of 15 pools, 7 rows and 8 columns, referred to as matrix peptide pool, was designed so that each peptide is in exactly one row-pool and one column-pool, thereby allowing for the identification of positive peptides at the intersection of positive pools. Matrix peptide pools that induced a CD4 T cell response (as determined by CD40L/CD154 assay described below) which meets the threshold criteria for a positive response were considered in the deconvolution process. Top six single peptides were considered for peptide-specific T cell expansion and sorting. A master peptide pool is composed of all of the 54 single peptides and was used to identify and sort total vaccine-specific CD4 T cells. Each peptide was used at a final concentration of 2 µg/ml. | ||||
| Funding: | 1. University of Antwerp [BOF Concerted Research Action (PS ID 30730) Methusalem funding; Industrial Research Fund SBO]. 2. Research Foundation Flanders (1861219N grant to B. Ogunjimi and FWO SB grant to N. De Neuter. | ||||
| Quality control: | Gates for CD40L+ CD4 T cells were set using cells left unstimulated. | ||||
Experiment variables
| Conditions | |
|---|---|
| · Negative Control | H02_Negative Control.fcs · H02_aNC.fcs · H03_aNC.fcs · H04_aNC.fcs · H06_aNC.fcs · H07f_Negative Control.fcs · H08f_Negative Control.fcs · H10_Negative Control.fcs · H11_Negative Control.fcs · H11_aNC.fcs · H13_Negative Control.fcs · H14_aNC.fcs · H17_Negative Control.fcs · H17_aNC.fcs · H18_Negative Control.fcs · H19_Negative Control.fcs · H20_Negative Control.fcs · H21_Negative Control.fcs · H21_aNC.fcs · H22_Negative Control.fcs · H23_aNC.fcs · H24_aNC.fcs · H25_aNC.fcs · H26_aNC.fcs · H28_Negative Control.fcs · H29_Negative Control.fcs · H30_aNC.fcs · H31_Negative Control.fcs · H32_Negative Control.fcs · H33_Negative Control.fcs · H34_Negative Control.fcs · H35_Negative Control.fcs · H36_Negative Control.fcs · H38_Negative Control.fcs · H38_aNC.fcs · H39_aNC.fcs · H40_Negative Control.fcs · H42_Negative Control.fcs · M41_aNC.fcs · M42_aNC.fcs |
| · Master Pool | H02_Master Pool.fcs · H07f_Master Pool.fcs · H08f_Master Pool.fcs · H10_Master Pool.fcs · H11_Master Pool.fcs · H13_Master Pool.fcs · H17_Master Pool.fcs · H18_Master Pool.fcs · H19_Master Pool.fcs · H20_Master Pool.fcs · H21_Master Pool.fcs · H22_Master Pool.fcs · H28_Master Pool.fcs · H29_Master Pool.fcs · H31_Master Pool.fcs · H32_Master Pool.fcs · H33_Master Pool.fcs · H34_Master Pool.fcs · H35_Master Pool.fcs · H36_Master Pool.fcs · H38_Master Pool.fcs · H40_Master Pool.fcs · H42_Master Pool.fcs |
| · MP01 | H02_MP01.fcs · H02_MP01.fcs-(1) · H03_MP01.fcs · H04_MP01.fcs · H06_MP01.fcs · H07f_MP01.fcs · H08f_MP01.fcs · H10_MP01.fcs · H11_MP01.fcs · H11_MP01.fcs-(1) · H13_MP01.fcs · H14_MP01.fcs · H17_MP01.fcs · H17_MP01.fcs-(1) · H18_MP01.fcs · H19_MP01.fcs · H20_MP01.fcs · H21_MP01.fcs · H21_MP01.fcs-(1) · H22_MP01.fcs · H23_MP01.fcs · H24_MP01.fcs · H25_MP01.fcs · H26_MP01.fcs · H28_MP01.fcs · H29_MP01.fcs · H30_MP01.fcs · H31_MP01.fcs · H32_MP01.fcs · H33_MP01.fcs · H34_MP01.fcs · H35_MP01.fcs · H36_MP01.fcs · H38_MP01.fcs · H38_MP01.fcs-(1) · H39_MP01.fcs · H40_MP01.fcs · H42_MP01.fcs · M41_MP01.fcs · M42_MP01.fcs |
| · MP02 | H02_MP02.fcs · H02_MP02.fcs-(1) · H03_MP02.fcs · H04_MP02.fcs · H06_MP02.fcs · H07f_MP02.fcs · H08f_MP02.fcs · H10_MP02.fcs · H11_MP02.fcs · H11_MP02.fcs-(1) · H13_MP02.fcs · H14_MP02.fcs · H17_MP02.fcs · H17_MP02.fcs-(1) · H18_MP02.fcs · H19_MP02.fcs · H20_MP02.fcs · H21_MP02.fcs · H21_MP02.fcs-(1) · H22_MP02.fcs · H23_MP02.fcs · H24_MP02.fcs · H25_MP02.fcs · H26_MP02.fcs · H28_MP02.fcs · H29_MP02.fcs · H30_MP02.fcs · H31_MP02.fcs · H32_MP02.fcs · H33_MP02.fcs · H34_MP02.fcs · H35_MP02.fcs · H36_MP02.fcs · H38_MP02.fcs · H38_MP02.fcs-(1) · H39_MP02.fcs · H40_MP02.fcs · H42_MP02.fcs · M41_MP02.fcs · M42_MP02.fcs |
| · MP03 | H02_MP03.fcs · H02_MP03.fcs-(1) · H03_MP03.fcs · H04_MP03.fcs · H06_MP03.fcs · H07f_MP03.fcs · H08f_MP03.fcs · H10_MP03.fcs · H11_MP03.fcs · H11_MP03.fcs-(1) · H13_MP03.fcs · H14_MP03.fcs · H17_MP03.fcs · H17_MP03.fcs-(1) · H18_MP03.fcs · H19_MP03.fcs · H20_MP03.fcs · H21_MP03.fcs · H21_MP03.fcs-(1) · H22_MP03.fcs · H23_MP03.fcs · H24_MP03.fcs · H25_MP03.fcs · H26_MP03.fcs · H28_MP03.fcs · H29_MP03.fcs · H30_MP03.fcs · H31_MP03.fcs · H32_MP03.fcs · H33_MP03.fcs · H34_MP03.fcs · H35_MP03.fcs · H36_MP03.fcs · H38_MP03.fcs · H38_MP03.fcs-(1) · H39_MP03.fcs · H40_MP03.fcs · H42_MP03.fcs · M41_MP03.fcs · M42_MP03.fcs |
| · MP04 | H02_MP04.fcs · H02_MP04.fcs-(1) · H03_MP04.fcs · H04_MP04.fcs · H06_MP04.fcs · H07f_MP04.fcs · H08f_MP04.fcs · H10_MP04.fcs · H11_MP04.fcs · H11_MP04.fcs-(1) · H13_MP04.fcs · H14_MP04.fcs · H17_MP04.fcs · H17_MP04.fcs-(1) · H18_MP04.fcs · H19_MP04.fcs · H20_MP04.fcs · H21_MP04.fcs · H21_MP04.fcs-(1) · H22_MP04.fcs · H23_MP04.fcs · H24_MP04.fcs · H25_MP04.fcs · H26_MP04.fcs · H28_MP04.fcs · H29_MP04.fcs · H30_MP04.fcs · H31_MP04.fcs · H32_MP04.fcs · H33_MP04.fcs · H34_MP04.fcs · H35_MP04.fcs · H36_MP04.fcs · H38_MP04.fcs · H38_MP04.fcs-(1) · H39_MP04.fcs · H40_MP04.fcs · H42_MP04.fcs · M41_MP04.fcs · M42_MP04.fcs |
| · MP05 | H02_MP05.fcs · H02_MP05.fcs-(1) · H03_MP05.fcs · H04_MP05.fcs · H06_MP05.fcs · H07f_MP05.fcs · H08f_MP05.fcs · H10_MP05.fcs · H11_MP05.fcs · H11_MP05.fcs-(1) · H13_MP05.fcs · H14_MP05.fcs · H17_MP05.fcs · H17_MP05.fcs-(1) · H18_MP05.fcs · H19_MP05.fcs · H20_MP05.fcs · H21_MP05.fcs · H21_MP05.fcs-(1) · H22_MP05.fcs · H23_MP05.fcs · H24_MP05.fcs · H25_MP05.fcs · H26_MP05.fcs · H28_MP05.fcs · H29_MP05.fcs · H30_MP05.fcs · H31_MP05.fcs · H32_MP05.fcs · H33_MP05.fcs · H34_MP05.fcs · H35_MP05.fcs · H36_MP05.fcs · H38_MP05.fcs · H38_MP05.fcs-(1) · H39_MP05.fcs · H40_MP05.fcs · H42_MP05.fcs · M41_MP05.fcs · M42_MP05.fcs |
| · MP06 | H02_MP06.fcs · H02_MP06.fcs-(1) · H03_MP06.fcs · H04_MP06.fcs · H06_MP06.fcs · H07f_MP06.fcs · H08f_MP06.fcs · H10_MP06.fcs · H11_MP06.fcs · H11_MP06.fcs-(1) · H13_MP06.fcs · H14_MP06.fcs · H17_MP06.fcs · H17_MP06.fcs-(1) · H18_MP06.fcs · H19_MP06.fcs · H20_MP06.fcs · H21_MP06.fcs · H21_MP06.fcs-(1) · H22_MP06.fcs · H23_MP06.fcs · H24_MP06.fcs · H25_MP06.fcs · H26_MP06.fcs · H28_MP06.fcs · H29_MP06.fcs · H30_MP06.fcs · H31_MP06.fcs · H32_MP06.fcs · H33_MP06.fcs · H34_MP06.fcs · H35_MP06.fcs · H36_MP06.fcs · H38_MP06.fcs · H38_MP06.fcs-(1) · H39_MP06.fcs · H40_MP06.fcs · H42_MP06.fcs · M41_MP06.fcs · M42_MP06.fcs |
| · MP07 | H02_MP07.fcs · H02_MP07.fcs-(1) · H03_MP07.fcs · H04_MP07.fcs · H06_MP07.fcs · H07f_MP07.fcs · H08f_MP07.fcs · H10_MP07.fcs · H11_MP07.fcs · H11_MP07.fcs-(1) · H13_MP07.fcs · H14_MP07.fcs · H17_MP07.fcs · H17_MP07.fcs-(1) · H18_MP07.fcs · H19_MP07.fcs · H20_MP07.fcs · H21_MP07.fcs · H21_MP07.fcs-(1) · H22_MP07.fcs · H23_MP07.fcs · H24_MP07.fcs · H25_MP07.fcs · H26_MP07.fcs · H28_MP07.fcs · H29_MP07.fcs · H30_MP07.fcs · H31_MP07.fcs · H32_MP07.fcs · H33_MP07.fcs · H34_MP07.fcs · H35_MP07.fcs · H36_MP07.fcs · H38_MP07.fcs · H38_MP07.fcs-(1) · H39_MP07.fcs · H40_MP07.fcs · H42_MP07.fcs · M41_MP07.fcs · M42_MP07.fcs |
| · MP08 | H02_MP08.fcs · H02_MP08.fcs-(1) · H03_MP08.fcs · H04_MP08.fcs · H06_MP08.fcs · H07f_MP08.fcs · H08f_MP08.fcs · H10_MP08.fcs · H11_MP08.fcs · H11_MP08.fcs-(1) · H13_MP08.fcs · H14_MP08.fcs · H17_MP08.fcs · H17_MP08.fcs-(1) · H18_MP08.fcs · H19_MP08.fcs · H20_MP08.fcs · H21_MP08.fcs · H21_MP08.fcs-(1) · H22_MP08.fcs · H23_MP08.fcs · H24_MP08.fcs · H25_MP08.fcs · H26_MP08.fcs · H28_MP08.fcs · H29_MP08.fcs · H30_MP08.fcs · H31_MP08.fcs · H32_MP08.fcs · H33_MP08.fcs · H34_MP08.fcs · H35_MP08.fcs · H36_MP08.fcs · H38_MP08.fcs · H38_MP08.fcs-(1) · H39_MP08.fcs · H40_MP08.fcs · H42_MP08.fcs · M41_MP08.fcs · M42_MP08.fcs |
| · MP09 | H02_MP09.fcs · H02_MP09.fcs-(1) · H03_MP09.fcs · H04_MP09.fcs · H06_MP09.fcs · H07f_MP09.fcs · H08f_MP09.fcs · H10_MP09.fcs · H11_MP09.fcs · H11_MP09.fcs-(1) · H13_MP09.fcs · H14_MP09.fcs · H17_MP09.fcs · H17_MP09.fcs-(1) · H18_MP09.fcs · H19_MP09.fcs · H20_MP09.fcs · H21_MP09.fcs · H21_MP09.fcs-(1) · H22_MP09.fcs · H23_MP09.fcs · H24_MP09.fcs · H25_MP09.fcs · H26_MP09.fcs · H28_MP09.fcs · H29_MP09.fcs · H30_MP09.fcs · H31_MP09.fcs · H32_MP09.fcs · H33_MP09.fcs · H34_MP09.fcs · H35_MP09.fcs · H36_MP09.fcs · H38_MP09.fcs · H38_MP09.fcs-(1) · H39_MP09.fcs · H40_MP09.fcs · H42_MP09.fcs · M41_MP09.fcs · M42_MP09.fcs |
| · MP10 | H02_MP10.fcs · H02_MP10.fcs-(1) · H03_MP10.fcs · H04_MP10.fcs · H06_MP10.fcs · H07f_MP10.fcs · H08f_MP10.fcs · H10_MP10.fcs · H11_MP10.fcs · H11_MP10.fcs-(1) · H13_MP10.fcs · H14_MP10.fcs · H17_MP10.fcs · H17_MP10.fcs-(1) · H18_MP10.fcs · H19_MP10.fcs · H20_MP10.fcs · H21_MP10.fcs · H21_MP10.fcs-(1) · H22_MP10.fcs · H23_MP10.fcs · H24_MP10.fcs · H25_MP10.fcs · H26_MP10.fcs · H28_MP10.fcs · H29_MP10.fcs · H30_MP10.fcs · H31_MP10.fcs · H32_MP10.fcs · H33_MP10.fcs · H34_MP10.fcs · H35_MP10.fcs · H36_MP10.fcs · H38_MP10.fcs · H38_MP10.fcs-(1) · H39_MP10.fcs · H40_MP10.fcs · H42_MP10.fcs · M41_MP10.fcs · M42_MP10.fcs |
| · MP11 | H02_MP11.fcs · H02_MP11.fcs-(1) · H03_MP11.fcs · H04_MP11.fcs · H06_MP11.fcs · H07f_MP11.fcs · H08f_MP11.fcs · H10_MP11.fcs · H11_MP11.fcs · H11_MP11.fcs-(1) · H13_MP11.fcs · H14_MP11.fcs · H17_MP11.fcs · H17_MP11.fcs-(1) · H18_MP11.fcs · H19_MP11.fcs · H20_MP11.fcs · H21_MP11.fcs · H21_MP11.fcs-(1) · H22_MP11.fcs · H23_MP11.fcs · H24_MP11.fcs · H25_MP11.fcs · H26_MP11.fcs · H28_MP11.fcs · H29_MP11.fcs · H30_MP11.fcs · H31_MP11.fcs · H32_MP11.fcs · H33_MP11.fcs · H34_MP11.fcs · H35_MP11.fcs · H36_MP11.fcs · H38_MP11.fcs · H38_MP11.fcs-(1) · H39_MP11.fcs · H40_MP11.fcs · H42_MP11.fcs · M41_MP11.fcs · M42_MP11.fcs |
| · MP12 | H02_MP12.fcs · H02_MP12.fcs-(1) · H03_MP12.fcs · H04_MP12.fcs · H06_MP12.fcs · H07f_MP12.fcs · H08f_MP12.fcs · H10_MP12.fcs · H11_MP12.fcs · H11_MP12.fcs-(1) · H13_MP12.fcs · H14_MP12.fcs · H17_MP12.fcs · H17_MP12.fcs-(1) · H18_MP12.fcs · H19_MP12.fcs · H20_MP12.fcs · H21_MP12.fcs · H21_MP12.fcs-(1) · H22_MP12.fcs · H23_MP12.fcs · H24_MP12.fcs · H25_MP12.fcs · H26_MP12.fcs · H28_MP12.fcs · H29_MP12.fcs · H30_MP12.fcs · H31_MP12.fcs · H32_MP12.fcs · H33_MP12.fcs · H34_MP12.fcs · H35_MP12.fcs · H36_MP12.fcs · H38_MP12.fcs · H38_MP12.fcs-(1) · H39_MP12.fcs · H40_MP12.fcs · H42_MP12.fcs · M41_MP12.fcs · M42_MP12.fcs |
| · MP13 | H02_MP13.fcs · H02_MP13.fcs-(1) · H03_MP13.fcs · H04_MP13.fcs · H06_MP13.fcs · H07f_MP13.fcs · H08f_MP13.fcs · H10_MP13.fcs · H11_MP13.fcs · H11_MP13.fcs-(1) · H13_MP13.fcs · H14_MP13.fcs · H17_MP13.fcs · H17_MP13.fcs-(1) · H18_MP13.fcs · H19_MP13.fcs · H20_MP13.fcs · H21_MP13.fcs · H21_MP13.fcs-(1) · H22_MP13.fcs · H23_MP13.fcs · H24_MP13.fcs · H25_MP13.fcs · H26_MP13.fcs · H28_MP13.fcs · H29_MP13.fcs · H30_MP13.fcs · H31_MP13.fcs · H32_MP13.fcs · H33_MP13.fcs · H34_MP13.fcs · H35_MP13.fcs · H36_MP13.fcs · H38_MP13.fcs · H38_MP13.fcs-(1) · H39_MP13.fcs · H40_MP13.fcs · H42_MP13.fcs · M41_MP13.fcs · M42_MP13.fcs |
| · MP14 | H02_MP14.fcs · H02_MP14.fcs-(1) · H03_MP14.fcs · H04_MP14.fcs · H06_MP14.fcs · H07f_MP14.fcs · H08f_MP14.fcs · H10_MP14.fcs · H11_MP14.fcs · H11_MP14.fcs-(1) · H13_MP14.fcs · H14_MP14.fcs · H17_MP14.fcs · H17_MP14.fcs-(1) · H18_MP14.fcs · H19_MP14.fcs · H20_MP14.fcs · H21_MP14.fcs · H21_MP14.fcs-(1) · H22_MP14.fcs · H23_MP14.fcs · H24_MP14.fcs · H25_MP14.fcs · H26_MP14.fcs · H28_MP14.fcs · H29_MP14.fcs · H30_MP14.fcs · H31_MP14.fcs · H32_MP14.fcs · H33_MP14.fcs · H34_MP14.fcs · H35_MP14.fcs · H36_MP14.fcs · H38_MP14.fcs · H38_MP14.fcs-(1) · H39_MP14.fcs · H40_MP14.fcs · H42_MP14.fcs · M41_MP14.fcs · M42_MP14.fcs |
| · MP15 | H02_MP15.fcs · H02_MP15.fcs-(1) · H03_MP15.fcs · H04_MP15.fcs · H06_MP15.fcs · H07f_MP15.fcs · H08f_MP15.fcs · H10_MP15.fcs · H11_MP15.fcs · H11_MP15.fcs-(1) · H13_MP15.fcs · H14_MP15.fcs · H17_MP15.fcs · H17_MP15.fcs-(1) · H18_MP15.fcs · H19_MP15.fcs · H20_MP15.fcs · H21_MP15.fcs · H21_MP15.fcs-(1) · H22_MP15.fcs · H23_MP15.fcs · H24_MP15.fcs · H25_MP15.fcs · H26_MP15.fcs · H28_MP15.fcs · H29_MP15.fcs · H30_MP15.fcs · H31_MP15.fcs · H32_MP15.fcs · H33_MP15.fcs · H34_MP15.fcs · H35_MP15.fcs · H36_MP15.fcs · H38_MP15.fcs · H38_MP15.fcs-(1) · H39_MP15.fcs · H40_MP15.fcs · H42_MP15.fcs · M41_MP15.fcs · M42_MP15.fcs |
| · Positive Control | H28_Positive Control.fcs · H29_Positive Control.fcs · H31_Positive Control.fcs · H32_Positive Control.fcs · H33_Positive Control.fcs · H34_Positive Control.fcs · H35_Positive Control.fcs · H36_Positive Control.fcs · H38_Positive Control.fcs · H40_Positive Control.fcs |
| Individuals | |
|---|---|
| · H02 | H02_MP01.fcs · H02_MP01.fcs-(1) · H02_MP02.fcs · H02_MP02.fcs-(1) · H02_MP03.fcs · H02_MP03.fcs-(1) · H02_MP04.fcs · H02_MP04.fcs-(1) · H02_MP05.fcs · H02_MP05.fcs-(1) · H02_MP06.fcs · H02_MP06.fcs-(1) · H02_MP07.fcs · H02_MP07.fcs-(1) · H02_MP08.fcs · H02_MP08.fcs-(1) · H02_MP09.fcs · H02_MP09.fcs-(1) · H02_MP10.fcs · H02_MP10.fcs-(1) · H02_MP11.fcs · H02_MP11.fcs-(1) · H02_MP12.fcs · H02_MP12.fcs-(1) · H02_MP13.fcs · H02_MP13.fcs-(1) · H02_MP14.fcs · H02_MP14.fcs-(1) · H02_MP15.fcs · H02_MP15.fcs-(1) · H02_Master Pool.fcs · H02_Negative Control.fcs · H02_aNC.fcs |
| · H03 | H03_MP01.fcs · H03_MP02.fcs · H03_MP03.fcs · H03_MP04.fcs · H03_MP05.fcs · H03_MP06.fcs · H03_MP07.fcs · H03_MP08.fcs · H03_MP09.fcs · H03_MP10.fcs · H03_MP11.fcs · H03_MP12.fcs · H03_MP13.fcs · H03_MP14.fcs · H03_MP15.fcs · H03_aNC.fcs |
| · H04 | H04_MP01.fcs · H04_MP02.fcs · H04_MP03.fcs · H04_MP04.fcs · H04_MP05.fcs · H04_MP06.fcs · H04_MP07.fcs · H04_MP08.fcs · H04_MP09.fcs · H04_MP10.fcs · H04_MP11.fcs · H04_MP12.fcs · H04_MP13.fcs · H04_MP14.fcs · H04_MP15.fcs · H04_aNC.fcs |
| · H06 | H06_MP01.fcs · H06_MP02.fcs · H06_MP03.fcs · H06_MP04.fcs · H06_MP05.fcs · H06_MP06.fcs · H06_MP07.fcs · H06_MP08.fcs · H06_MP09.fcs · H06_MP10.fcs · H06_MP11.fcs · H06_MP12.fcs · H06_MP13.fcs · H06_MP14.fcs · H06_MP15.fcs · H06_aNC.fcs |
| · H07 | H07f_MP01.fcs · H07f_MP02.fcs · H07f_MP03.fcs · H07f_MP04.fcs · H07f_MP05.fcs · H07f_MP06.fcs · H07f_MP07.fcs · H07f_MP08.fcs · H07f_MP09.fcs · H07f_MP10.fcs · H07f_MP11.fcs · H07f_MP12.fcs · H07f_MP13.fcs · H07f_MP14.fcs · H07f_MP15.fcs · H07f_Master Pool.fcs · H07f_Negative Control.fcs |
| · H08 | H08f_MP01.fcs · H08f_MP02.fcs · H08f_MP03.fcs · H08f_MP04.fcs · H08f_MP05.fcs · H08f_MP06.fcs · H08f_MP07.fcs · H08f_MP08.fcs · H08f_MP09.fcs · H08f_MP10.fcs · H08f_MP11.fcs · H08f_MP12.fcs · H08f_MP13.fcs · H08f_MP14.fcs · H08f_MP15.fcs · H08f_Master Pool.fcs · H08f_Negative Control.fcs |
| · H10 | H10_MP01.fcs · H10_MP02.fcs · H10_MP03.fcs · H10_MP04.fcs · H10_MP05.fcs · H10_MP06.fcs · H10_MP07.fcs · H10_MP08.fcs · H10_MP09.fcs · H10_MP10.fcs · H10_MP11.fcs · H10_MP12.fcs · H10_MP13.fcs · H10_MP14.fcs · H10_MP15.fcs · H10_Master Pool.fcs · H10_Negative Control.fcs |
| · H11 | H11_MP01.fcs · H11_MP01.fcs-(1) · H11_MP02.fcs · H11_MP02.fcs-(1) · H11_MP03.fcs · H11_MP03.fcs-(1) · H11_MP04.fcs · H11_MP04.fcs-(1) · H11_MP05.fcs · H11_MP05.fcs-(1) · H11_MP06.fcs · H11_MP06.fcs-(1) · H11_MP07.fcs · H11_MP07.fcs-(1) · H11_MP08.fcs · H11_MP08.fcs-(1) · H11_MP09.fcs · H11_MP09.fcs-(1) · H11_MP10.fcs · H11_MP10.fcs-(1) · H11_MP11.fcs · H11_MP11.fcs-(1) · H11_MP12.fcs · H11_MP12.fcs-(1) · H11_MP13.fcs · H11_MP13.fcs-(1) · H11_MP14.fcs · H11_MP14.fcs-(1) · H11_MP15.fcs · H11_MP15.fcs-(1) · H11_Master Pool.fcs · H11_Negative Control.fcs · H11_aNC.fcs |
| · H13 | H13_MP01.fcs · H13_MP02.fcs · H13_MP03.fcs · H13_MP04.fcs · H13_MP05.fcs · H13_MP06.fcs · H13_MP07.fcs · H13_MP08.fcs · H13_MP09.fcs · H13_MP10.fcs · H13_MP11.fcs · H13_MP12.fcs · H13_MP13.fcs · H13_MP14.fcs · H13_MP15.fcs · H13_Master Pool.fcs · H13_Negative Control.fcs |
| · H14 | H14_MP01.fcs · H14_MP02.fcs · H14_MP03.fcs · H14_MP04.fcs · H14_MP05.fcs · H14_MP06.fcs · H14_MP07.fcs · H14_MP08.fcs · H14_MP09.fcs · H14_MP10.fcs · H14_MP11.fcs · H14_MP12.fcs · H14_MP13.fcs · H14_MP14.fcs · H14_MP15.fcs · H14_aNC.fcs |
| · H17 | H17_MP01.fcs · H17_MP01.fcs-(1) · H17_MP02.fcs · H17_MP02.fcs-(1) · H17_MP03.fcs · H17_MP03.fcs-(1) · H17_MP04.fcs · H17_MP04.fcs-(1) · H17_MP05.fcs · H17_MP05.fcs-(1) · H17_MP06.fcs · H17_MP06.fcs-(1) · H17_MP07.fcs · H17_MP07.fcs-(1) · H17_MP08.fcs · H17_MP08.fcs-(1) · H17_MP09.fcs · H17_MP09.fcs-(1) · H17_MP10.fcs · H17_MP10.fcs-(1) · H17_MP11.fcs · H17_MP11.fcs-(1) · H17_MP12.fcs · H17_MP12.fcs-(1) · H17_MP13.fcs · H17_MP13.fcs-(1) · H17_MP14.fcs · H17_MP14.fcs-(1) · H17_MP15.fcs · H17_MP15.fcs-(1) · H17_Master Pool.fcs · H17_Negative Control.fcs · H17_aNC.fcs |
| · H18 | H18_MP01.fcs · H18_MP02.fcs · H18_MP03.fcs · H18_MP04.fcs · H18_MP05.fcs · H18_MP06.fcs · H18_MP07.fcs · H18_MP08.fcs · H18_MP09.fcs · H18_MP10.fcs · H18_MP11.fcs · H18_MP12.fcs · H18_MP13.fcs · H18_MP14.fcs · H18_MP15.fcs · H18_Master Pool.fcs · H18_Negative Control.fcs |
| · H19 | H19_MP01.fcs · H19_MP02.fcs · H19_MP03.fcs · H19_MP04.fcs · H19_MP05.fcs · H19_MP06.fcs · H19_MP07.fcs · H19_MP08.fcs · H19_MP09.fcs · H19_MP10.fcs · H19_MP11.fcs · H19_MP12.fcs · H19_MP13.fcs · H19_MP14.fcs · H19_MP15.fcs · H19_Master Pool.fcs · H19_Negative Control.fcs |
| · H20 | H20_MP01.fcs · H20_MP02.fcs · H20_MP03.fcs · H20_MP04.fcs · H20_MP05.fcs · H20_MP06.fcs · H20_MP07.fcs · H20_MP08.fcs · H20_MP09.fcs · H20_MP10.fcs · H20_MP11.fcs · H20_MP12.fcs · H20_MP13.fcs · H20_MP14.fcs · H20_MP15.fcs · H20_Master Pool.fcs · H20_Negative Control.fcs |
| · H21 | H21_MP01.fcs · H21_MP01.fcs-(1) · H21_MP02.fcs · H21_MP02.fcs-(1) · H21_MP03.fcs · H21_MP03.fcs-(1) · H21_MP04.fcs · H21_MP04.fcs-(1) · H21_MP05.fcs · H21_MP05.fcs-(1) · H21_MP06.fcs · H21_MP06.fcs-(1) · H21_MP07.fcs · H21_MP07.fcs-(1) · H21_MP08.fcs · H21_MP08.fcs-(1) · H21_MP09.fcs · H21_MP09.fcs-(1) · H21_MP10.fcs · H21_MP10.fcs-(1) · H21_MP11.fcs · H21_MP11.fcs-(1) · H21_MP12.fcs · H21_MP12.fcs-(1) · H21_MP13.fcs · H21_MP13.fcs-(1) · H21_MP14.fcs · H21_MP14.fcs-(1) · H21_MP15.fcs · H21_MP15.fcs-(1) · H21_Master Pool.fcs · H21_Negative Control.fcs · H21_aNC.fcs |
| · H22 | H22_MP01.fcs · H22_MP02.fcs · H22_MP03.fcs · H22_MP04.fcs · H22_MP05.fcs · H22_MP06.fcs · H22_MP07.fcs · H22_MP08.fcs · H22_MP09.fcs · H22_MP10.fcs · H22_MP11.fcs · H22_MP12.fcs · H22_MP13.fcs · H22_MP14.fcs · H22_MP15.fcs · H22_Master Pool.fcs · H22_Negative Control.fcs |
| · H23 | H23_MP01.fcs · H23_MP02.fcs · H23_MP03.fcs · H23_MP04.fcs · H23_MP05.fcs · H23_MP06.fcs · H23_MP07.fcs · H23_MP08.fcs · H23_MP09.fcs · H23_MP10.fcs · H23_MP11.fcs · H23_MP12.fcs · H23_MP13.fcs · H23_MP14.fcs · H23_MP15.fcs · H23_aNC.fcs |
| · H24 | H24_MP01.fcs · H24_MP02.fcs · H24_MP03.fcs · H24_MP04.fcs · H24_MP05.fcs · H24_MP06.fcs · H24_MP07.fcs · H24_MP08.fcs · H24_MP09.fcs · H24_MP10.fcs · H24_MP11.fcs · H24_MP12.fcs · H24_MP13.fcs · H24_MP14.fcs · H24_MP15.fcs · H24_aNC.fcs |
| · H25 | H25_MP01.fcs · H25_MP02.fcs · H25_MP03.fcs · H25_MP04.fcs · H25_MP05.fcs · H25_MP06.fcs · H25_MP07.fcs · H25_MP08.fcs · H25_MP09.fcs · H25_MP10.fcs · H25_MP11.fcs · H25_MP12.fcs · H25_MP13.fcs · H25_MP14.fcs · H25_MP15.fcs · H25_aNC.fcs |
| · H26 | H26_MP01.fcs · H26_MP02.fcs · H26_MP03.fcs · H26_MP04.fcs · H26_MP05.fcs · H26_MP06.fcs · H26_MP07.fcs · H26_MP08.fcs · H26_MP09.fcs · H26_MP10.fcs · H26_MP11.fcs · H26_MP12.fcs · H26_MP13.fcs · H26_MP14.fcs · H26_MP15.fcs · H26_aNC.fcs |
| · H28 | H28_MP01.fcs · H28_MP02.fcs · H28_MP03.fcs · H28_MP04.fcs · H28_MP05.fcs · H28_MP06.fcs · H28_MP07.fcs · H28_MP08.fcs · H28_MP09.fcs · H28_MP10.fcs · H28_MP11.fcs · H28_MP12.fcs · H28_MP13.fcs · H28_MP14.fcs · H28_MP15.fcs · H28_Master Pool.fcs · H28_Negative Control.fcs · H28_Positive Control.fcs |
| · H29 | H29_MP01.fcs · H29_MP02.fcs · H29_MP03.fcs · H29_MP04.fcs · H29_MP05.fcs · H29_MP06.fcs · H29_MP07.fcs · H29_MP08.fcs · H29_MP09.fcs · H29_MP10.fcs · H29_MP11.fcs · H29_MP12.fcs · H29_MP13.fcs · H29_MP14.fcs · H29_MP15.fcs · H29_Master Pool.fcs · H29_Negative Control.fcs · H29_Positive Control.fcs |
| · H30 | H30_MP01.fcs · H30_MP02.fcs · H30_MP03.fcs · H30_MP04.fcs · H30_MP05.fcs · H30_MP06.fcs · H30_MP07.fcs · H30_MP08.fcs · H30_MP09.fcs · H30_MP10.fcs · H30_MP11.fcs · H30_MP12.fcs · H30_MP13.fcs · H30_MP14.fcs · H30_MP15.fcs · H30_aNC.fcs |
| · H31 | H31_MP01.fcs · H31_MP02.fcs · H31_MP03.fcs · H31_MP04.fcs · H31_MP05.fcs · H31_MP06.fcs · H31_MP07.fcs · H31_MP08.fcs · H31_MP09.fcs · H31_MP10.fcs · H31_MP11.fcs · H31_MP12.fcs · H31_MP13.fcs · H31_MP14.fcs · H31_MP15.fcs · H31_Master Pool.fcs · H31_Negative Control.fcs · H31_Positive Control.fcs |
| · H32 | H32_MP01.fcs · H32_MP02.fcs · H32_MP03.fcs · H32_MP04.fcs · H32_MP05.fcs · H32_MP06.fcs · H32_MP07.fcs · H32_MP08.fcs · H32_MP09.fcs · H32_MP10.fcs · H32_MP11.fcs · H32_MP12.fcs · H32_MP13.fcs · H32_MP14.fcs · H32_MP15.fcs · H32_Master Pool.fcs · H32_Negative Control.fcs · H32_Positive Control.fcs |
| · H33 | H33_MP01.fcs · H33_MP02.fcs · H33_MP03.fcs · H33_MP04.fcs · H33_MP05.fcs · H33_MP06.fcs · H33_MP07.fcs · H33_MP08.fcs · H33_MP09.fcs · H33_MP10.fcs · H33_MP11.fcs · H33_MP12.fcs · H33_MP13.fcs · H33_MP14.fcs · H33_MP15.fcs · H33_Master Pool.fcs · H33_Negative Control.fcs · H33_Positive Control.fcs |
| · H34 | H34_MP01.fcs · H34_MP02.fcs · H34_MP03.fcs · H34_MP04.fcs · H34_MP05.fcs · H34_MP06.fcs · H34_MP07.fcs · H34_MP08.fcs · H34_MP09.fcs · H34_MP10.fcs · H34_MP11.fcs · H34_MP12.fcs · H34_MP13.fcs · H34_MP14.fcs · H34_MP15.fcs · H34_Master Pool.fcs · H34_Negative Control.fcs · H34_Positive Control.fcs |
| · H35 | H35_MP01.fcs · H35_MP02.fcs · H35_MP03.fcs · H35_MP04.fcs · H35_MP05.fcs · H35_MP06.fcs · H35_MP07.fcs · H35_MP08.fcs · H35_MP09.fcs · H35_MP10.fcs · H35_MP11.fcs · H35_MP12.fcs · H35_MP13.fcs · H35_MP14.fcs · H35_MP15.fcs · H35_Master Pool.fcs · H35_Negative Control.fcs · H35_Positive Control.fcs |
| · H36 | H36_MP01.fcs · H36_MP02.fcs · H36_MP03.fcs · H36_MP04.fcs · H36_MP05.fcs · H36_MP06.fcs · H36_MP07.fcs · H36_MP08.fcs · H36_MP09.fcs · H36_MP10.fcs · H36_MP11.fcs · H36_MP12.fcs · H36_MP13.fcs · H36_MP14.fcs · H36_MP15.fcs · H36_Master Pool.fcs · H36_Negative Control.fcs · H36_Positive Control.fcs |
| · H38 | H38_MP01.fcs · H38_MP01.fcs-(1) · H38_MP02.fcs · H38_MP02.fcs-(1) · H38_MP03.fcs · H38_MP03.fcs-(1) · H38_MP04.fcs · H38_MP04.fcs-(1) · H38_MP05.fcs · H38_MP05.fcs-(1) · H38_MP06.fcs · H38_MP06.fcs-(1) · H38_MP07.fcs · H38_MP07.fcs-(1) · H38_MP08.fcs · H38_MP08.fcs-(1) · H38_MP09.fcs · H38_MP09.fcs-(1) · H38_MP10.fcs · H38_MP10.fcs-(1) · H38_MP11.fcs · H38_MP11.fcs-(1) · H38_MP12.fcs · H38_MP12.fcs-(1) · H38_MP13.fcs · H38_MP13.fcs-(1) · H38_MP14.fcs · H38_MP14.fcs-(1) · H38_MP15.fcs · H38_MP15.fcs-(1) · H38_Master Pool.fcs · H38_Negative Control.fcs · H38_Positive Control.fcs · H38_aNC.fcs |
| · H39 | H39_MP01.fcs · H39_MP02.fcs · H39_MP03.fcs · H39_MP04.fcs · H39_MP05.fcs · H39_MP06.fcs · H39_MP07.fcs · H39_MP08.fcs · H39_MP09.fcs · H39_MP10.fcs · H39_MP11.fcs · H39_MP12.fcs · H39_MP13.fcs · H39_MP14.fcs · H39_MP15.fcs · H39_aNC.fcs |
| · H40 | H40_MP01.fcs · H40_MP02.fcs · H40_MP03.fcs · H40_MP04.fcs · H40_MP05.fcs · H40_MP06.fcs · H40_MP07.fcs · H40_MP08.fcs · H40_MP09.fcs · H40_MP10.fcs · H40_MP11.fcs · H40_MP12.fcs · H40_MP13.fcs · H40_MP14.fcs · H40_MP15.fcs · H40_Master Pool.fcs · H40_Negative Control.fcs · H40_Positive Control.fcs |
| · H42 | H42_MP01.fcs · H42_MP02.fcs · H42_MP03.fcs · H42_MP04.fcs · H42_MP05.fcs · H42_MP06.fcs · H42_MP07.fcs · H42_MP08.fcs · H42_MP09.fcs · H42_MP10.fcs · H42_MP11.fcs · H42_MP12.fcs · H42_MP13.fcs · H42_MP14.fcs · H42_MP15.fcs · H42_Master Pool.fcs · H42_Negative Control.fcs |
