Experiment Overview
| Repository ID: | FR-FCM-Z2VX | Experiment name: | Rhinovirus Challenge Study_TREX | MIFlowCyt score: | 68.50% |
| Primary researcher: | Lyndsey Muehling | PI/manager: | Lyndsey Muehling | Uploaded by: | Lyndsey Muehling |
| Experiment dates: | 2020-09-14 - | Dataset uploaded: | Sep 2020 | Last updated: | Aug 2021 |
| Keywords: | [CD4 T cells] [MHC multimers] [rhinovirus] | Manuscripts: |
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| Organizations: |
University of Virginia, Charlottesville, VA (USA)
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| Purpose: | Healthy adult volunteers were experimentally challenged with RV-A16. PBMCs were monitored longitudinally in order to track CD4+ T cell responses to infection. Rhinovirus specificity was determined by staining with MHCII tetramers. | ||||
| Conclusion: | Analysis with T-REX reveals that in infected subjects, those cells with the greatest change from pre-infection (day 0) to acute infection (day 7) time points are enriched for rhinovirus tetramer+ cells. | ||||
| Comments: | None | ||||
| Funding: | NIH U01 AI125056 | ||||
| Quality control: | All data were unmixed using single-stained reference controls, and tetramer and phenotypic gates were identified using FMO controls. All samples from one subject were analyzed in the same experiment. | ||||
Experiment variables
| Individuals | |
|---|---|
| · RV001 | 1_pre_day-14.fcs_CD4.fcs · 1_pre_day0.fcs_CD4.fcs · 1_pre_day28.fcs_CD4.fcs · 1_pre_day7.fcs_CD4.fcs |
| · RV003 | 3_Pre_day-14.fcs_CD4.fcs · 3_Pre_day0.fcs_CD4.fcs · 3_Pre_day28.fcs_CD4.fcs · 3_Pre_day7.fcs_CD4.fcs |
| · RV002 | 2_Pre_day-14.fcs_CD4.fcs · 2_Pre_day0.fcs_CD4.fcs · 2_Pre_day28.fcs_CD4.fcs · 2_Pre_day7.fcs_CD4.fcs |
| · RV004 | 4_D-14_Pre-enriched.fcs_CD4.fcs · 4_D0_Pre-enriched.fcs_CD4.fcs · 4_D28_Pre-enriched.fcs_CD4.fcs · 4_D7_Pre-enriched.fcs_CD4.fcs |
| · RV005 | 5_D-14_Pre-enriched.fcs_CD4.fcs · 5_D0_Pre-enriched.fcs_CD4.fcs · 5_D28_Pre-enriched.fcs_CD4.fcs · 5_D7_Pre-enriched.fcs_CD4.fcs |
| · RV006 | 6_Pre_day-14.fcs_CD4.fcs · 6_Pre_day0.fcs_CD4.fcs · 6_Pre_day28.fcs_CD4.fcs · 6_Pre_day7.fcs_CD4.fcs |
| · RV007 | 7_Pre_day-14.fcs_CD4.fcs · 7_Pre_day0.fcs_CD4.fcs · 7_Pre_day28_CD4.fcs · 7_Pre_day7.fcs_CD4.fcs |
| · RV008 | 8_pre_day-14.fcs_CD4.fcs · 8_pre_day0_CD4-(1).fcs · 8_pre_day28.fcs_CD4.fcs · 8_pre_day7.fcs_CD4.fcs |
| Timepoints | |
|---|---|
| · D-14 | 1_pre_day-14.fcs_CD4.fcs · 3_Pre_day-14.fcs_CD4.fcs · 2_Pre_day-14.fcs_CD4.fcs · 4_D-14_Pre-enriched.fcs_CD4.fcs · 5_D-14_Pre-enriched.fcs_CD4.fcs · 6_Pre_day-14.fcs_CD4.fcs · 7_Pre_day-14.fcs_CD4.fcs · 8_pre_day-14.fcs_CD4.fcs |
| · D0 | 1_pre_day0.fcs_CD4.fcs · 3_Pre_day0.fcs_CD4.fcs · 2_Pre_day0.fcs_CD4.fcs · 4_D0_Pre-enriched.fcs_CD4.fcs · 5_D0_Pre-enriched.fcs_CD4.fcs · 6_Pre_day0.fcs_CD4.fcs · 7_Pre_day0.fcs_CD4.fcs · 8_pre_day0_CD4-(1).fcs |
| · D7 | 1_pre_day7.fcs_CD4.fcs · 3_Pre_day7.fcs_CD4.fcs · 2_Pre_day7.fcs_CD4.fcs · 4_D7_Pre-enriched.fcs_CD4.fcs · 5_D7_Pre-enriched.fcs_CD4.fcs · 6_Pre_day7.fcs_CD4.fcs · 7_Pre_day7.fcs_CD4.fcs · 8_pre_day7.fcs_CD4.fcs |
| · D28 | 1_pre_day28.fcs_CD4.fcs · 3_Pre_day28.fcs_CD4.fcs · 2_Pre_day28.fcs_CD4.fcs · 4_D28_Pre-enriched.fcs_CD4.fcs · 5_D28_Pre-enriched.fcs_CD4.fcs · 6_Pre_day28.fcs_CD4.fcs · 7_Pre_day28_CD4.fcs · 8_pre_day28.fcs_CD4.fcs |
