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Experiment Overview

Repository ID: FR-FCM-Z3GH Experiment name: COVID PREGNANT MIFlowCyt score: 47.75%
Primary researcher: Sara De Biasi PI/manager: Sara De Biasi Uploaded by: Sara De Biasi
Experiment dates: 2020-08-03 - 2021-02-01 Dataset uploaded: Feb 2021 Last updated: Apr 2021
Keywords: [cytokines] [CyTOF] [COVID-19] [pregnant] Manuscripts:
Organizations: University of Modena and Reggio Emilia, Modena, (Italy)
Purpose: To investigate PBMC landscape of Pregnant women with with COVID-19 infection.
Conclusion: Unsupervised analyses revealed that most immune parameters were similar in patients and uninfected controls, apart from an increase in low density neutrophils in SARS-CoV-2 positive women. Also, patients did not show altered plasma levels of interleukin-6 or other main inflammatory molecules, but displayed significant increases of anti-inflammatory cytokines such as IL-1RA, IL-10 and IL-19, and decreased levels of IL-17, PD-L1 and D-dimer. The control of inflammation, as evidenced by soluble molecule levels, could be a possible strategy used during pregnancy to avoid virus-induced damages and maintain a normal immune response.
Comments: In 14 pregnant women who had asymptomatic or mild SARS-CoV-2 infection, we performed a detailed 38-parameter analysis of peripheral blood mononuclear cells by mass cytometry, studied the expression of T-cell master regular genes, investigated cell proliferation and cytokine production, and measured plasma levels of 62 cytokines. No patient showed lymphopenia or gross alterations of white blood cells.
Funding: This study also received unrestricted donations from Glem Gas spa (San Cesario, Modena, Italy), Sanfelice 1893 Banca Popolare (San Felice S.P., Modena, Italy) and Rotary Club Distretto 2072 (Clubs: Modena, Modena L.A. Muratori, Carpi, Sassuolo, Castelvetro di Modena), C.O.F.I.M. spa & Gianni Gibellini, Franco Appari, Andrea Lucchi, Federica Vagnarelli, Biogas Europa Service & Massimo Faccia, Pierangelo Bertoli Fans Club and Alberto Bertoli, Maria Santoro, Valentina Spezzani and BPER Banca. Finally, special thanks to the patients who donated blood to participate to this study.
Quality control: human PBMC in COVID-19
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