Experiment Overview
| Repository ID: | FR-FCM-Z3PD | Experiment name: | Comparison of CD19 sgRNA vs pgRNA targeting efficiency | MIFlowCyt score: | 98.00% |
| Primary researcher: | Carme Arnan | PI/manager: | Carme Arnan | Uploaded by: | Carme Arnan |
| Experiment dates: | 2016-12-13 - 2016-12-13 | Dataset uploaded: | Apr 2021 | Last updated: | May 2022 |
| Keywords: | [CRISPR] [CD19] [sgRNA] [pgRNA] | Manuscripts: | |||
| Organizations: |
Cytometry Unit Facility (CRG/UPF), Barcelona, Catalonia (SPAIN)
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| Purpose: | comparission of CRISPR-Cas9 targeting CD19 using sgRNA vs pgRNA | ||||
| Conclusion: | Targeting CD19 with CRISPR-Cas9 and pgRNAs is more efficient than targeting with sgRNA. | ||||
| Comments: | None | ||||
| Funding: | N/A | ||||
| Quality control: | Checked BlaER-Cas9 as cells with 100% CD19 signal | ||||
Experiment variables
| Timepoints | |
|---|---|
| · T0 | 20161213_Blaer 788.fcs · 20161213_Blaer 815.fcs · 20161213_Blaer 816.fcs · 20161213_Blaer 817.fcs · 20161213_Blaer 818.fcs · 20161213_Blaer 819.fcs · 20161213_Blaer 820.fcs · 20161213_Blaer 821.fcs · 20161213_Blaer 822.fcs · 20161213_Blaer 823.fcs · 20161213_Blaer 824.fcs · 20161213_Blaer 825.fcs |
| Sample Type | |
|---|---|
| · BLaER-Cas9 | 20161213_Blaer 788.fcs · 20161213_Blaer 815.fcs · 20161213_Blaer 816.fcs · 20161213_Blaer 817.fcs · 20161213_Blaer 818.fcs · 20161213_Blaer 819.fcs · 20161213_Blaer 820.fcs · 20161213_Blaer 821.fcs · 20161213_Blaer 822.fcs · 20161213_Blaer 823.fcs · 20161213_Blaer 824.fcs · 20161213_Blaer 825.fcs |
| Conditions | |
|---|---|
| · Samples | 20161213_Blaer 815.fcs · 20161213_Blaer 816.fcs · 20161213_Blaer 817.fcs · 20161213_Blaer 818.fcs · 20161213_Blaer 819.fcs · 20161213_Blaer 820.fcs · 20161213_Blaer 821.fcs · 20161213_Blaer 822.fcs · 20161213_Blaer 823.fcs · 20161213_Blaer 824.fcs · 20161213_Blaer 825.fcs |
| · Controls | 20161213_Blaer 788.fcs |
