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Experiment Overview

Repository ID: FR-FCM-Z3PH Experiment name: CRISPR library targeting lncRNA and protein coding genes along BLaER1 transdifferentiation (Replicate 2) MIFlowCyt score: 98.00%
Primary researcher: Carme Arnan PI/manager: Carme Arnan Uploaded by: Carme Arnan
Experiment dates: 2018-03-02 - 2018-03-02 Dataset uploaded: Apr 2021 Last updated: May 2022
Keywords: [macrophages] [CRISPR-Cas9 screening ] [BLaER cells] [pre-B cells] [transdifferentiation] Manuscripts:
Organizations: Cytometry Unit Facility (CRG/UPF), Barcelona, Catalonia (SPAIN)
Purpose: CRISPR library screening experiment in BLaER-Cas9 cells that were induced to transdifferentiate into macrophages. The purpose of the experiment is to discover novel players (lncRNAs and protein-coding genes) in the B-cell to macrophage transdifferentiation process.
Conclusion: There is a delayed population of cells that do not differentiate normally (this population is separated by cell sorting) in comparission to a control experiment with cells infected with a intergenic pgRNA
Comments: The experiment was done in 2 biological resplicates (R1 and R2).
Funding: N/A
Quality control: BLaER Cas9 infected with non-targeting pgRNA negative control.


Experiment variables

Timepoints
· T0 20180302_10 Lib T0.fcs · 20180302_8 Blaer T0.fcs · 20180302_9 S T0.fcs
· T3 20180302_11 Blaer T3.fcs · 20180302_12 S T3.fcs · 20180302_13 Lib T3.fcs · 20180302_17 ERC Lib T3 sort.fcs
· T6 20180302_14 Blaer 6D.fcs · 20180302_15 S T6.fcs · 20180302_16 ERC Lib T6.fcs · 20180302_18 ERC Lib T6 sort.fcs

Conditions
· Controls 20180302_11 Blaer T3.fcs · 20180302_12 S T3.fcs · 20180302_14 Blaer 6D.fcs · 20180302_15 S T6.fcs · 20180302_8 Blaer T0.fcs · 20180302_9 S T0.fcs
· Samples 20180302_10 Lib T0.fcs · 20180302_13 Lib T3.fcs · 20180302_16 ERC Lib T6.fcs · 20180302_17 ERC Lib T3 sort.fcs · 20180302_18 ERC Lib T6 sort.fcs

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