Experiment Overview
| Repository ID: | FR-FCM-Z3PJ | Experiment name: | Validation of candidate hits along BLaER1 transdifferentiation (Replicate 2) | MIFlowCyt score: | 84.00% |
| Primary researcher: | Carme Arnan | PI/manager: | Carme Arnan | Uploaded by: | Carme Arnan |
| Experiment dates: | 2019-01-18 - 2019-01-21 | Dataset uploaded: | Apr 2021 | Last updated: | May 2022 |
| Keywords: | [macrophages] [transdifferentiation] [Target validation] [pc-genes] [lncRNAs] [BLaER (pre-B cells)] | Manuscripts: | |||
| Organizations: |
Cytometry Unit Facility (CRG/UPF), Barcelona, Catalonia (SPAIN)
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| Purpose: | Validation of hits from CRISPR-Cas9 screening library in BLaER cells (replicate 2). | ||||
| Conclusion: | 5 protein coding genes(CEACAM, FURIN, KLF4, NFE2 and TREML2) and 2 lncRNAs (LINC02432 and MIR3945HG) were individually validated. | ||||
| Comments: | None | ||||
| Funding: | N/A | ||||
| Quality control: | BLaER Cas9 infected with Intergenic pgRNA. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · T0 | 20190121_10 Furin 870 t0.fcs · 20190121_11 KLF4 873 t0.fcs · 20190121_12 Unk 874 t0.fcs · 20190121_13 Spi1 875 t0.fcs · 20190121_14 CEACAM 876 t0.fcs · 20190121_15 NFE 877 t0.fcs · 20190121_16 TREML 878 t0.fcs · 20190121_19 881 t0.fcs · 20190121_20 882 t0.fcs · 20190121_8 Blaer Cas9 t0.fcs · 20190121_9 rat cebpa 869 t0.fcs · 20190118_10 Furin 870 t0.fcs · 20190118_11 KLF4 873 t0.fcs · 20190118_12 Unk 874 t0.fcs · 20190118_13 Spi1 875 t0.fcs · 20190118_14 CEACAM 876 t0.fcs · 20190118_15 NFE 877 t0.fcs · 20190118_16 TREML 878 t0.fcs · 20190118_19 881 t0.fcs · 20190118_20 882 t0.fcs · 20190118_8 Blaer Cas9 t0.fcs · 20190118_9 rat cebpa 869 t0.fcs |
| · T3 | 20190118_22 Blaer Cas9 t3.fcs · 20190118_23 rat cebpa 869 t3.fcs · 20190118_24 Furin 870 t3.fcs · 20190118_25 KLF4873 t3.fcs · 20190118_26 Unk 874 t3.fcs · 20190118_27 Spi1 875 t3.fcs · 20190118_28 CEACAM 876 t3.fcs · 20190118_29 NFE 877 t3.fcs · 20190118_30 TREML 878 t3.fcs · 20190118_33 881 t3.fcs · 20190118_34 882 t3.fcs |
| · T6 | 20190121_22 Blaer Cas9 t6.fcs · 20190121_23 rat cebpa 869 t6.fcs · 20190121_24 Furin 870 t6.fcs · 20190121_25 KLF4873 t6.fcs · 20190121_26 Unk 874 t6.fcs · 20190121_27 Spi1 875 t6.fcs · 20190121_28 CEACAM 876 t6.fcs · 20190121_29 NFE 877 t6.fcs · 20190121_30 TREML 878 t6.fcs · 20190121_33 881 t6 No MAc1 antib.fcs · 20190121_34 882 t6 No Mac1 antib.fcs |
| Conditions | |
|---|---|
| · Protein coding genes | 20190121_10 Furin 870 t0.fcs · 20190121_11 KLF4 873 t0.fcs · 20190121_14 CEACAM 876 t0.fcs · 20190121_15 NFE 877 t0.fcs · 20190121_16 TREML 878 t0.fcs · 20190121_24 Furin 870 t6.fcs · 20190121_25 KLF4873 t6.fcs · 20190121_28 CEACAM 876 t6.fcs · 20190121_29 NFE 877 t6.fcs · 20190121_30 TREML 878 t6.fcs · 20190118_10 Furin 870 t0.fcs · 20190118_11 KLF4 873 t0.fcs · 20190118_14 CEACAM 876 t0.fcs · 20190118_15 NFE 877 t0.fcs · 20190118_16 TREML 878 t0.fcs · 20190118_24 Furin 870 t3.fcs · 20190118_25 KLF4873 t3.fcs · 20190118_28 CEACAM 876 t3.fcs · 20190118_29 NFE 877 t3.fcs · 20190118_30 TREML 878 t3.fcs |
| · lncRNAs | 20190121_19 881 t0.fcs · 20190121_20 882 t0.fcs · 20190121_33 881 t6 No MAc1 antib.fcs · 20190121_34 882 t6 No Mac1 antib.fcs · 20190118_19 881 t0.fcs · 20190118_20 882 t0.fcs · 20190118_33 881 t3.fcs · 20190118_34 882 t3.fcs |
| · positive controls | 20190121_13 Spi1 875 t0.fcs · 20190121_23 rat cebpa 869 t6.fcs · 20190121_27 Spi1 875 t6.fcs · 20190121_9 rat cebpa 869 t0.fcs · 20190118_13 Spi1 875 t0.fcs · 20190118_23 rat cebpa 869 t3.fcs · 20190118_27 Spi1 875 t3.fcs · 20190118_9 rat cebpa 869 t0.fcs |
| · negative controls | 20190121_12 Unk 874 t0.fcs · 20190121_22 Blaer Cas9 t6.fcs · 20190121_26 Unk 874 t6.fcs · 20190121_8 Blaer Cas9 t0.fcs · 20190118_12 Unk 874 t0.fcs · 20190118_22 Blaer Cas9 t3.fcs · 20190118_26 Unk 874 t3.fcs · 20190118_8 Blaer Cas9 t0.fcs |
