Experiment Overview
| Repository ID: | FR-FCM-Z3PL | Experiment name: | Validation of GapmeR targeted lncRNAs along BLaER1 transdifferentiation | MIFlowCyt score: | 98.00% |
| Primary researcher: | Carme Arnan | PI/manager: | Carme Arnan | Uploaded by: | Carme Arnan |
| Experiment dates: | 2019-04-15 - 2019-04-15 | Dataset uploaded: | Apr 2021 | Last updated: | May 2022 |
| Keywords: | [macrophages] [gapmers] [pre-B cells] [transdifferentiation] [lncRNA expression] [BLaER cell] | Manuscripts: | |||
| Organizations: |
Cytometry Unit Facility (CRG/UPF), Barcelona, Catalonia (SPAIN)
|
||||
| Purpose: | Checking the effect of GapmeRs in knocking down lncRNAs in BLaER cells. | ||||
| Conclusion: | Knockdown of lncRNAs does not effect the transdifferentiation of BLaER cell line. | ||||
| Comments: | It was checked the knockdown effect of GapmeRs against lncRNAs at RNA level (checked by qPCR). | ||||
| Funding: | Not disclosed | ||||
| Quality control: | A negative control GapmeR was used. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · T3 | 190415_GFP t3.fcs · 190415_Gapmer 881 t3.fcs · 190415_Gapmer 882 t3.fcs · 190415_Gapmer C- t3.fcs |
| · T0 | 190415_GFP.fcs |
| Conditions | |
|---|---|
| · Controls | 190415_GFP t3.fcs · 190415_GFP.fcs · 190415_Gapmer C- t3.fcs |
| · Samples | 190415_Gapmer 881 t3.fcs · 190415_Gapmer 882 t3.fcs |
