Experiment Overview

Repository ID: FR-FCM-Z5FK Experiment name: p-S6 rescue experiment in Vim-/- MEFs MIFlowCyt score: 65.75%
Primary researcher: Ponnuswamy Mohanasundaram PI/manager: Ponnuswamy Mohanasundaram Uploaded by: Ponnuswamy Mohanasundaram
Experiment dates: 2021-08-01 - 2022-11-30 Dataset uploaded: Jun 2022 Last updated: Jun 2022
Keywords: [cell size] [mTOR] [Vimenitn] Manuscripts:
Organizations: Åbo Akademi, Åbo Akademi, Turku, (Finland)
Purpose: WT Vimentin was transfected in Vim-/- MEFs to rescue the p-S6 serine 234/235 level. '
Conclusion: Transfection of WT Vimentin rescued p-S6 serine 234/235 level in Vim-/- MEFs.
Comments: p-S6 fluorescent intensity was measured in WT and Vim-/- MEFs steady-state. Vim-/- MEFs were transfected with WT Vimentin, then cells expressing Vimentin and nontransfected cells were measured for P-S6 fluorescent intensity after starvation and stimulation (essential amino acids, glucose, and insulin).
Funding: Academy of Finland
Quality control: Vimentin was labeled with Alexa 647 and phospho-S6 was labeled with Alexa 488. Cells labeled with vimentin Alexa 647 alone and cells labeled with pS6 Alexa 488 alone were also used. 'Data was acquired with the same setting for all samples. Then phospho-S6 fluorescent intensity was measured in vimentin-positive and vimentin null gated populations.

Experiment variables

· Steady state Specimen_001_B6_B06_006.fcs · Specimen_001_B8_B08_008.fcs · Specimen_001_B7_B07_007.fcs · Specimen_001_B9_B09_009.fcs · Specimen_001_C6_C06_006.fcs · Specimen_001_C8_C08_008.fcs
· Stravation Specimen_001_C10_C10_021.fcs · Specimen_001_C1_C01_011.fcs · Specimen_001_C5_C05_015.fcs · Specimen_001_C9_C09_019.fcs · Specimen_001_C10_C10_010.fcs
· Stimulation Specimen_001_C12_C12_023.fcs · Specimen_001_C11_C11_021.fcs · Specimen_001_C3_C03_013.fcs · Specimen_001_C7_C07_017.fcs · Specimen_001_C12_C12_012.fcs

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