Experiment Overview
| Repository ID: | FR-FCM-Z5R6 | Experiment name: | Exosome-induced changes of E-Cadherin and Vimentin expression by HNSCC cell line | MIFlowCyt score: | 51.50% |
| Primary researcher: | Linda Hofmann | PI/manager: | Linda Hofmann | Uploaded by: | Linda Hofmann |
| Experiment dates: | 2019-06-01 - 2019-08-31 | Dataset uploaded: | Oct 2022 | Last updated: | Oct 2022 |
| Keywords: | [exosomes] [EMT] [HNSCC] | Manuscripts: | |||
| Organizations: |
Ulm University, ENT Department, Ulm, (Germany)
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| Purpose: | Reveal influence of patient-derived exosomes from before and after therapy on the epithelial and mesenchymal protein profile of tumor cells in vitro | ||||
| Conclusion: | Cells treated with exosomes from both timepoints showed a reduced E-cadherin expression compared to untreated cells. However, exosomes from patients after therapy induced a significantly lower E-cadherin inhibition compared to exosomes from patients before therapy. Cells co-incubated with exosomes from patients before and after therapy showed an increase of vimentin expression compared to untreated cells, however non-significant. | ||||
| Comments: | None | ||||
| Funding: | NA | ||||
| Quality control: | Flow-Check™ Pro Fluorospheres - Beckman Coulter | ||||
Experiment variables
| Conditions | |
|---|---|
| · before therapy | Pat02_BT_E-Cadherin_FITC.fcs · Pat02_BT_Isotype_FITC.fcs · Pat03_BT_E-Cadherin_FITC.fcs · Pat03_BT_Isotype_FITC.fcs · Pat04_BT_E-Cadherin_FITC.fcs · Pat04_BT_Isotype_FITC.fcs · Pat05_BT_E-Cadherin_FITC.fcs · Pat05_BT_Isotype_FITC.fcs · Pat06_BT_E-Cadherin_FITC.fcs · Pat06_BT_Isotype_FITC.fcs · Pat08_BT_E-Cadherin_FITC.fcs · Pat08_BT_Isotype_FITC.fcs · Pat09_BT_E-Cadherin_FITC.fcs · Pat09_BT_Isotype_FITC.fcs · Pat10_BT_E-Cadherin_FITC.fcs · Pat10_BT_Isotype_FITC.fcs · Pat11_BT_E-Cadherin_FITC.fcs · Pat11_BT_Isotype_FITC.fcs · Pat12_BT_E-Cadherin_FITC.fcs · Pat12_BT_Isotype_FITC.fcs · Pat13_BT_E-Cadherin_FITC.fcs · Pat13_BT_Isotype_FITC.fcs · Pat14_BT_E-Cadherin_FITC.fcs · Pat14_BT_Isotype_FITC.fcs · Pat15_BT_E-Cadherin_FITC.fcs · Pat15_BT_Isotype_FITC.fcs · Pat16_BT_E-Cadherin_FITC.fcs · Pat16_BT_Isotype_FITC.fcs · Pat17_BT_E-Cadherin_FITC.fcs · Pat17_BT_Isotype_FITC.fcs · Pat18_BT_E-Cadherin_FITC.fcs · Pat18_BT_Isotype_FITC.fcs · Pat19_BT_E-Cadherin_FITC.fcs · Pat19_BT_Isotype_FITC.fcs · Pat01_BT_E-Cadherin_FITC.fcs · Pat01_BT_Isotype_FITC.fcs · Pat02_BT_Isotype_AF488.fcs · Pat02_BT_Vimentin_488.fcs · Pat04_BT_Isotype_AF488.fcs · Pat04_BT_Vimentin_488.fcs · Pat05_BT_Isotype_AF488.fcs · Pat05_BT_Vimentin_488.fcs · Pat06_BT_Isotype_AF488.fcs · Pat06_BT_Vimentin_488.fcs · Pat08_BT_Isotype_AF488.fcs · Pat08_BT_Vimentin_488.fcs · Pat09_BT_Isotype_AF488.fcs · Pat09_BT_Vimentin_488.fcs · Pat10_BT_Isotype_AF488.fcs · Pat10_BT_Vimentin_488.fcs · Pat11_BT_Isotype_AF488.fcs · Pat11_BT_Vimentin_488.fcs · Pat12_BT_Isotype_AF488.fcs · Pat12_BT_Vimentin_488.fcs · Pat13_BT_Isotype_AF488.fcs · Pat13_BT_Vimentin_488.fcs · Pat14_BT_Isotype_AF488.fcs · Pat14_BT_Vimentin_488.fcs · Pat15_BT_Isotype_AF488.fcs · Pat15_BT_Vimentin_488.fcs · Pat16_BT_Isotype_AF488.fcs · Pat16_BT_Vimentin_488.fcs · Pat17_BT_Isotype_AF488.fcs · Pat17_BT_Vimentin_488.fcs · Pat18_BT_Isotype_AF488.fcs · Pat18_BT_Vimentin_488.fcs · Pat19_BT_Isotype_AF488.fcs · Pat19_BT_Vimentin_488.fcs |
| · after therapy | Pat02_AT_E-Cadherin_FITC.fcs · Pat02_AT_Isotype_FITC.fcs · Pat03_AT_E-Cadherin_FITC.fcs · Pat03_AT_Isotype_FITC.fcs · Pat04_AT_E-Cadherin_FITC.fcs · Pat04_AT_Isotype_FITC.fcs · Pat05_AT_E-Cadherin_FITC.fcs · Pat05_AT_Isotype_FITC.fcs · Pat06_AT_E-Cadherin_FITC.fcs · Pat06_AT_Isotype_FITC.fcs · Pat07_AT_E-Cadherin_FITC.fcs · Pat07_AT_Isotype_FITC.fcs · Pat08_AT_E-Cadherin_FITC.fcs · Pat08_AT_Isotype_FITC.fcs · Pat09_AT_E-Cadherin_FITC.fcs · Pat09_AT_Isotype_FITC.fcs · Pat10_AT_E-Cadherin_FITC.fcs · Pat10_AT_Isotype_FITC.fcs · Pat11_AT_E-Cadherin_FITC.fcs · Pat11_AT_Isotype_FITC.fcs · Pat12_AT_E-Cadherin_FITC.fcs · Pat12_AT_Isotype_FITC.fcs · Pat13_AT_E-Cadherin_FITC.fcs · Pat13_AT_Isotype_FITC.fcs · Pat14_AT_E-Cadherin_FITC.fcs · Pat14_AT_Isotype_FITC.fcs · Pat15_AT_E-Cadherin_FITC.fcs · Pat15_AT_Isotype_FITC.fcs · Pat16_AT_E-Cadherin_FITC.fcs · Pat16_AT_Isotype_FITC.fcs · Pat17_AT_E-Cadherin_FITC.fcs · Pat17_AT_Isotype_FITC.fcs · Pat18_AT_E-Cadherin_FITC.fcs · Pat18_AT_Isotype_FITC.fcs · Pat19_AT_E-Cadherin_FITC.fcs · Pat19_AT_Isotype_FITC.fcs · Pat01_AT_E-Cadherin_FITC.fcs · Pat01_AT_Isotype_FITC.fcs · Pat02_AT_Isotype_AF488.fcs · Pat02_AT_Vimentin_488.fcs · Pat03_AT_Isotype_AF488.fcs · Pat03_AT_Vimentin_488.fcs · Pat04_AT_Isotype_AF488.fcs · Pat04_AT_Vimentin_488.fcs · Pat05_AT_Isotype_AF488.fcs · Pat05_AT_Vimentin_488.fcs · Pat06_AT_Isotype_AF488.fcs · Pat06_AT_Vimentin_488.fcs · Pat07_AT_Isotype_AF488.fcs · Pat07_AT_Vimentin_488.fcs · Pat08_AT_Isotype_AF488.fcs · Pat08_AT_Vimentin_488.fcs · Pat09_AT_Isotype_AF488.fcs · Pat09_AT_Vimentin_488.fcs · Pat10_AT_Isotype_AF488.fcs · Pat10_AT_Vimentin_488.fcs · Pat11_AT_Isotype_AF488.fcs · Pat11_AT_Vimentin_488.fcs · Pat12_AT_Isotype_AF488.fcs · Pat12_AT_Vimentin_488.fcs · Pat13_AT_Isotype_AF488.fcs · Pat13_AT_Vimentin_488.fcs · Pat14_AT_Isotype_AF488.fcs · Pat14_AT_Vimentin_488.fcs · Pat15_AT_Isotype_AF488.fcs · Pat15_AT_Vimentin_488.fcs · Pat16_AT_Isotype_AF488.fcs · Pat16_AT_Vimentin_488.fcs · Pat17_AT_Isotype_AF488.fcs · Pat17_AT_Vimentin_488.fcs · Pat18_AT_Isotype_AF488.fcs · Pat18_AT_Vimentin_488.fcs · Pat19_AT_Isotype_AF488.fcs · Pat19_AT_Vimentin_488.fcs |
