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Experiment Overview

Repository ID: FR-FCM-Z5ZP Experiment name: Analysis of TC-mAb mice (Exp3: B1-B, B2-B) MIFlowCyt score: 30.75%
Primary researcher: Takashi Moriwaki PI/manager: Yasuhiro Kazuki Uploaded by: Takashi Moriwaki
Experiment dates: 2021-04-21 - 2021-05-18 Dataset uploaded: Feb 2022 Last updated: Mar 2022
Keywords: None Manuscripts:
Organizations: Tottori University, Yonago, (Japan)
Purpose: The experiment had been performed to analyze B cell development of human antibody-procducing mice (TC-mAb mice). The flow cytometry experiment was designed to detect the subsets of Follicular B (B220hiCD19+CD93-CD21loCD23hi), marginal zone B (B220hiCD19+CD93-CD21hiCD23lo), and B1a B (B220loCD19+CD5+CD43+) in Spleen of 6, 8, and 20 week-old mice, and B1a B (CD19+CD5+CD23-), B1b B (CD19+CD5-CD23-), B2 B (CD19+CD5lo/-CD23+) in peritoneal exudate cells of 6, 8, and 20 week-old mice.
Conclusion: None
Comments: This experiment corresponds to Figure 7d, e and Supplementary Figure 21-26 in the paper "Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci". Publication link: https://doi.org/10.21203/rs.3.rs-103241/v1
Funding: Not disclosed
Quality control: None
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