Experiment Overview
| Repository ID: | FR-FCM-Z5ZQ | Experiment name: | Analysis of TC-mAb mice (Exp4: Germinal center B cell) | MIFlowCyt score: | 30.68% |
| Primary researcher: | Takashi Moriwaki | PI/manager: | Yasuhiro Kazuki | Uploaded by: | Takashi Moriwaki |
| Experiment dates: | 2021-04-16 - 2021-07-02 | Dataset uploaded: | Feb 2022 | Last updated: | Mar 2022 |
| Keywords: | None | Manuscripts: | |||
| Organizations: |
Tottori University, Yonago, (Japan)
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| Purpose: | The experiment had been performed to analyze B cell development of human antibody-procducing mice (TC-mAb mice). The flow cytometry experiment was designed to detect the subsets of germinal center B (CD38lo/-GL7+),dark zone B (CXCR4hiCD86lo) and light zone B (CXCR4loCD86hi) in spleen of unimmunized mice and immunized mice at 7, 14, 21, and 28 days after immunization. | ||||
| Conclusion: | None | ||||
| Comments: | This experiment corresponds to Figure 8a, b, c and Supplementary Figure 27-29 in the paper "Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci". Publication link: https://doi.org/10.21203/rs.3.rs-103241/v1 | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
