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Experiment Overview

Repository ID: FR-FCM-Z5ZQ Experiment name: Analysis of TC-mAb mice (Exp4: Germinal center B cell) MIFlowCyt score: 30.68%
Primary researcher: Takashi Moriwaki PI/manager: Yasuhiro Kazuki Uploaded by: Takashi Moriwaki
Experiment dates: 2021-04-16 - 2021-07-02 Dataset uploaded: Feb 2022 Last updated: Mar 2022
Keywords: None Manuscripts:
Organizations: Tottori University, Yonago, (Japan)
Purpose: The experiment had been performed to analyze B cell development of human antibody-procducing mice (TC-mAb mice). The flow cytometry experiment was designed to detect the subsets of germinal center B (CD38lo/-GL7+),dark zone B (CXCR4hiCD86lo) and light zone B (CXCR4loCD86hi) in spleen of unimmunized mice and immunized mice at 7, 14, 21, and 28 days after immunization.
Conclusion: None
Comments: This experiment corresponds to Figure 8a, b, c and Supplementary Figure 27-29 in the paper "Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci". Publication link: https://doi.org/10.21203/rs.3.rs-103241/v1
Funding: Not disclosed
Quality control: None
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