Experiment Overview

Repository ID: FR-FCM-Z5ZU Experiment name: Analysis of TC-mAb mice (Exp7: Cell sorting of germinal center B cells) MIFlowCyt score: 50.75%
Primary researcher: Takashi Moriwaki PI/manager: Yasuhiro Kazuki Uploaded by: Takashi Moriwaki
Experiment dates: 2021-04-29 - 2021-06-17 Dataset uploaded: Feb 2022 Last updated: Mar 2022
Keywords: None Manuscripts:
Organizations: Tottori University, Yonago, (Japan)
Purpose: The experiment had been performed to isolate germinal center B cells for the analysis of the B cell receptor repertoires of human antibody-procducing mice (TC-mAb mice). The flow cytometry experiment was designed to detect the subsets of germinal center B (CD38lo/-GL7+) in the spleen of unimmunized mice and immunized mice at 14, 21 days after immunization.
Conclusion: None
Comments: This experiment corresponds to Supplementary Figure 35 in the paper "Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci". Publication link:
Funding: Not disclosed
Quality control: None
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