Experiment Overview
| Repository ID: | FR-FCM-Z5ZU | Experiment name: | Analysis of TC-mAb mice (Exp7: Cell sorting of germinal center B cells) | MIFlowCyt score: | 50.75% |
| Primary researcher: | Takashi Moriwaki | PI/manager: | Yasuhiro Kazuki | Uploaded by: | Takashi Moriwaki |
| Experiment dates: | 2021-04-29 - 2021-06-17 | Dataset uploaded: | Feb 2022 | Last updated: | Mar 2022 |
| Keywords: | None | Manuscripts: | |||
| Organizations: |
Tottori University, Yonago, (Japan)
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| Purpose: | The experiment had been performed to isolate germinal center B cells for the analysis of the B cell receptor repertoires of human antibody-procducing mice (TC-mAb mice). The flow cytometry experiment was designed to detect the subsets of germinal center B (CD38lo/-GL7+) in the spleen of unimmunized mice and immunized mice at 14, 21 days after immunization. | ||||
| Conclusion: | None | ||||
| Comments: | This experiment corresponds to Supplementary Figure 35 in the paper "Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci". Publication link: https://doi.org/10.21203/rs.3.rs-103241/v1 | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
