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Experiment Overview

Repository ID: FR-FCM-Z5ZW Experiment name: Analysis of TC-mAb mice (Exp2: Transitonal B, Mature B)) MIFlowCyt score: 30.66%
Primary researcher: Takashi Moriwaki PI/manager: Yasuhiro Kazuki Uploaded by: Takashi Moriwaki
Experiment dates: 2021-04-21 - 2021-04-28 Dataset uploaded: Feb 2022 Last updated: Mar 2022
Keywords: None Manuscripts:
Organizations: Tottori University, Yonago, (Japan)
Purpose: The experiment had been performed to analyze B cell development of human antibody-procducing mice (TC-mAb mice). The flow cytometry experiment was designed to detect the subsets of Follicular B (CD19+CD93-CD21loCD23hi), marginal zone B (CD19+CD93-CD21hiCD23lo) cells, transitional (T1 (CD19+CD93+IgMhiCD23-), T2 (CD19+CD93+IgMhiCD23+), and T3 (CD19+CD93+IgMloCD23+) in Spleen and Lymph node. In addditon, the expression pattern of IgM and IgD in Igk+ follicular B cells was also analyzed.
Conclusion: None
Comments: This experiment corresponds to Figure 7b, c and Supplementary Figure 17-20 in the paper "Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci". Publication link: https://doi.org/10.21203/rs.3.rs-103241/v1
Funding: Not disclosed
Quality control: None
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