Experiment Overview
| Repository ID: | FR-FCM-Z7TB | Experiment name: | PINK1 is a target of T cell responses in Parkinson?s disease | MIFlowCyt score: | 26.42% |
| Primary researcher: | Antoine Freuchet | PI/manager: | Antoine Freuchet | Uploaded by: | Antoine Freuchet |
| Experiment dates: | 2024-07-08 - | Dataset uploaded: | Jul 2024 | Last updated: | Jan 2025 |
| Keywords: | None | Manuscripts: | |||
| Organizations: |
La Jolla Institute for Immunology , La Jolla, CA (USA)
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| Purpose: | Determining the frequency of non-CD3, CD4 and CD8 T cells before and after a 2 weeks expansions with peptide pools. | ||||
| Conclusion: | Only CD4 T cell proportion greatly increased after culture. Non-CD3 decreases and CD8 T cells remain similar. | ||||
| Comments: | None | ||||
| Funding: | This work was partly supported by Aligning Science Across Parkinson’s (ASAP-000375 to CLA, DS) through the Michael J. Fox Foundation for Parkinson’s Research (MJFF). For open access, the authors have applied a CC-BY public copyright license to all Author Accepted Manuscripts arising from this submission. This work was also supported by the NIH T32AI125179 (GPW), the National Institute of Neurological Disorders and Stroke of the NIH R01NS095435 (AS, DS), the JPB Foundation (DS), and the WHAM Investigator’s fund (CLA). | ||||
| Quality control: | None | ||||
Experiment variables
| Conditions | |
|---|---|
| · Pre-stimulation | donor 1 6102_stained.fcs · donor 1 6750_stained.fcs · donor 2 6002_stained.fcs · donor 2 6053_stained.fcs · donor 3 6101_stained.fcs · donor 3 6229_stained.fcs |
| · Post-stimulation (14 days expansion) | Donor 3 6101_PINK1.fcs · donor 1 6102_PINK1.fcs · donor 1 6750_PINK1.fcs · donor 2 6002_PINK1.fcs · donor 2 6053_PINK1.fcs · donor 3 6229_PINK1.fcs |
| Individuals | |
|---|---|
| · 6101 | Donor 3 6101_PINK1.fcs · donor 3 6101_stained.fcs |
| · 6102 | donor 1 6102_PINK1.fcs · donor 1 6102_stained.fcs |
| · 6750 | donor 1 6750_PINK1.fcs · donor 1 6750_stained.fcs |
| · 6002 | donor 2 6002_PINK1.fcs · donor 2 6002_stained.fcs |
| · 6053 | donor 2 6053_PINK1.fcs · donor 2 6053_stained.fcs |
| · 6229 | donor 3 6229_PINK1.fcs · donor 3 6229_stained.fcs |
| Sample Type | |
|---|---|
| · PBMC | Donor 3 6101_PINK1.fcs · donor 1 6102_PINK1.fcs · donor 1 6102_stained.fcs · donor 1 6750_PINK1.fcs · donor 1 6750_stained.fcs · donor 2 6002_PINK1.fcs · donor 2 6002_stained.fcs · donor 2 6053_PINK1.fcs · donor 2 6053_stained.fcs · donor 3 6101_stained.fcs · donor 3 6229_PINK1.fcs · donor 3 6229_stained.fcs |
