Experiment Overview

Repository ID: FR-FCM-ZYLF Experiment name: Effect background fluorescence and spreading error in high-dimensional flow cytometry data visualization by tSNE MIFlowCyt score: 22.84%
Primary researcher: Jolanda Brummelman PI/manager: Enrico Lugli Uploaded by: Jolanda Brummelman
Experiment dates: 2017-04-04 - Dataset uploaded: Jun 2018 Last updated: Jun 2018
Keywords: [T cell] [CD8] [tSNE] [polychromatic flow cytometry] [single cell] [high-dimensional data] Manuscripts:
Organizations: None
Purpose: Testing the applicability of tSNE to 27-parameter single-cell flow cytometry data.
Conclusion: Background fluorescence and spreading error impact the visualisation of multidimensional single-cell data by tSNE substantially, specifically resulting in the generation of virtual “islands” that do not reflect real heterogeneity at the phenotypic or biological level.
Comments: None
Funding: Not disclosed
Quality control: None
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