Experiment Overview
| Repository ID: | FR-FCM-ZYNB | Experiment name: | Replication Study: The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44 | MIFlowCyt score: | 86.00% |
| Primary researcher: | Timothy Errington | PI/manager: | Timothy Errington | Uploaded by: | Timothy Errington |
| Experiment dates: | 2016-02-17 - 2018-01-19 | Dataset uploaded: | Jul 2018 | Last updated: | Jun 2019 |
| Keywords: | [replication] [reproducibility] [metascience] [Reproducibility Project: Cancer Biology] [miR-34a] [prostate cancer] | Manuscripts: | [30860027] [PMC6414201] |
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| Organizations: |
Center for Open Science, Charlottesville, VA (United States of America)
Science Exchange, Palo Alto, CA (United States of America) Crown Biosciences, Inc, Taicang City, Jiangsu Province (P.R. China) |
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| Purpose: | This experiment assesses the expression of miR-34a and let-7b in CD44+ and CD44- populations from human tumor cells purified from xenograft tumors formed in male NOD/SCID mice that were injected with LAPC4 prostate cancer cells. This protocol replicates the experiment reported in Figure 1B of Liu et al., 2011. | ||||
| Conclusion: | We found the microRNA, miR-34a, was expressed at twice the level in CD44+ prostate cancer cells purified from xenograft tumors (LAPC4 cells) compared to CD44- LAPC4 cells, whereas the original study reported miR-34a was underexpressed in CD44+ LAPC4 cells (Figure 1B; Liu et al., 2011). Let-7b expression was underexpressed in CD44+ cells in both studies. | ||||
| Comments: | This is experiment is part of the Reproducibility Project: Cancer Biology (https://osf.io/e81xl/wiki/home/). The Reproducibility Project: Cancer Biology (RP:CB) is a collaboration between the Center for Open Science and Science Exchange that seeks to address concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. | ||||
| Funding: | The Reproducibility Project: Cancer Biology is funded by the Laura and John Arnold Foundation, provided to the Center for Open Science in collaboration with Science Exchange. The funder had no role in study design, data collection and interpretation, or the decision to submit the work for publication. | ||||
| Quality control: | The cell lines used in this experiment underwent STR profiling to confirm their identity and sent for mycoplasma testing to ensure there is no contamination. An isotype control antibody was used to confirm the specificity of the CD44 antibody. Additionally, human peripheral blood mononuclear cells (PBMC) were also tested to confirm specificity of the antibodies and staining procedure. Additional detailed experimental notes, data, and analysis are available on the Open Science Framework (OSF) (https://osf.io/gb7sr/) | ||||
Experiment variables
| Sample Type | |
|---|---|
| · LAPC4 cells | Specimen_001_CON.fcs · Specimen_001_FITC.fcs |
| Conditions | |
|---|---|
| · none | Specimen_001_CON.fcs · Specimen_001_FITC.fcs |
