Experiment Overview
| Repository ID: | FR-FCM-ZYWP | Experiment name: | Effects of Mosquito Saliva on Human PBMCs | MIFlowCyt score: | 18.25% |
| Primary researcher: | Megan Vogt | PI/manager: | Megan Vogt | Uploaded by: | Megan Vogt |
| Experiment dates: | 2016-04-08 - | Dataset uploaded: | Apr 2018 | Last updated: | Apr 2018 |
| Keywords: | None | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | Mosquito saliva is thought to have immunomodulatory effects on human immune cells. To test this, we stimulated human PBMCs (from two separate human donors) with mosquito saliva obtained from Aedes aegypti mosquitoes and performed flow cytometry to determine changes in immune cell populations at day 5 post stimulation. Media stimulation was used as a negative control and pokeweed mitogen and LPS were used as positive controls. | ||||
| Conclusion: | None | ||||
| Comments: | Data were acquired on a LSRII Fortessa using HTS attachment. Data were analyzed using flow jo v10. Compensation was performed in Flowjo. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Single color controls using ultra e-comp beads for voltage checks and compensation. Fluorescence minus one controls using PBMCs for gating controls. | ||||
