Experiment Overview

Repository ID: FR-FCM-ZZC8 Experiment name: CD4 and INFg mRNA/Protein expression in stimulated PBMC MIFlowCyt score: 100.00%
Primary researcher: Chip Lomas PI/manager: Emily Park Uploaded by: Chip Lomas
Experiment dates: 2014-03-31 - 2014-04-01 Dataset uploaded: May 2014 Last updated: Aug 2014
Keywords: [PBMC] [CD4] [Gene expression] [stimulated] [RNA Flow cytometry] Manuscripts: [25123411] Cytalogo
Organizations: BD Biosciences, Research and Development, San Jose, CA (USA)
Purpose: The purpose was to demonstrate the effect of a 5 hour PMA/Ionomycin stimulation on expression of IFNg mRNA, CD4 mRNA, CD4 protein, and IFNg protein in lymphocytes.
Conclusion: After a 5 hour PMA/Ionomycin treatment, expression of CD4 protein and mRNA is decreased, while both IFNg protein and mRNA is increased.
Comments: Figure 2A-F and supplemental: Simultaneous flow cytometric analysis of IFN-g and CD4 mRNA and protein expression kinetics in human peripheral blood mononuclear cells during activation
Funding: Not disclosed
Quality control: Cytometer setup using CST in FACSDiva software.

Experiment variables

Sample Type
· PBMC PBMC_Stim 5 hr no Blocker_003.fcs · PBMC_Unstim 5hr no Blocker_001.fcs
· PBMC no target probe added negative control PBMC_NTC Stim 5hr no Blocker_004.fcs · PBMC_NTC Unstim 5hr no Blocker_002.fcs

· 5 hour PMA/Ionomycin PBMC_NTC Stim 5hr no Blocker_004.fcs · PBMC_Stim 5 hr no Blocker_003.fcs
· 5 hour rest PBMC_NTC Unstim 5hr no Blocker_002.fcs · PBMC_Unstim 5hr no Blocker_001.fcs

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