Download-button_s

Experiment Overview

Repository ID: FR-FCM-ZZCA Experiment name: Monocyte CD4 and IFNg mRNA expression kinetics in stimulated PBMCs MIFlowCyt score: 100.00%
Primary researcher: Chip Lomas PI/manager: Emily Park Uploaded by: Chip Lomas
Experiment dates: 2014-03-31 - 2014-04-01 Dataset uploaded: May 2014 Last updated: Aug 2014
Keywords: [CD44] [monocyte] [PBMC] [Gene expression] [RNA Flow cytometry] Manuscripts: [25123411] Cytalogo
Organizations: BD Biosciences, Research and Development, San Jose, CA (USA)
Purpose: The purpose of this experiment was to determine the effect of PMA/Ionocmycin stimulation on the monocyte population in PBMCs.
Conclusion: CD4 downregulation occurs in the lymphocyte subset.
Comments: Figure 3 D-L: Simultaneous flow cytometric analysis of IFN-g and CD4 mRNA and protein expression kinetics in human peripheral blood mononuclear cells during activation
Funding: Not disclosed
Quality control: Instrument was setup with CST in FACSDiva software.


Experiment variables

Timepoints
· 0 min PBMC Stim_NTC T0_004.fcs · PBMC Stim_T0_001.fcs
· 120 min PBMC Stim_120 min PMA,2f,Ionomycin_002.fcs
· 300 min PBMC Stim_5 hr PMA,2f,Ionomycin_003.fcs · PBMC Stim_NTC 5 hr_005.fcs

Sample Type
· PBMC PBMC Stim_120 min PMA,2f,Ionomycin_002.fcs · PBMC Stim_5 hr PMA,2f,Ionomycin_003.fcs · PBMC Stim_T0_001.fcs
· PBMC no target probe added negative control PBMC Stim_NTC 5 hr_005.fcs · PBMC Stim_NTC T0_004.fcs

Conditions
· PMA/Ionomycin Stimulation PBMC Stim_120 min PMA,2f,Ionomycin_002.fcs · PBMC Stim_5 hr PMA,2f,Ionomycin_003.fcs · PBMC Stim_NTC 5 hr_005.fcs · PBMC Stim_NTC T0_004.fcs · PBMC Stim_T0_001.fcs

Download FCS Files or login and see the dataset in your inbox for further annotation details.