Experiment Overview
| Repository ID: | FR-FCM-ZYLA | Experiment name: | Heterogeneity of circulating CD8 T-cells specific to islet, neo-antigen and virus in patients with type 1 diabetes mellitus. | MIFlowCyt score: | 28.50% |
| Primary researcher: | Sandra Laban | PI/manager: | Sandra Laban | Uploaded by: | Sandra Laban |
| Experiment dates: | 2015-02-23 - 2016-06-28 | Dataset uploaded: | Jun 2018 | Last updated: | Jun 2018 |
| Keywords: | [mass cytometry] [CyTOF] [autoreactive cells] [CD8 T-cells] [Tetramers] [type 1 diabetes] | Manuscripts: | |||
| Organizations: |
LUMC, IHB, Leiden, (The Netherlands)
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| Purpose: | Auto-reactive CD8 T-cells play an important role in the destruction of pancreatic beta cells resulting in type 1 diabetes (T1D). We used high-dimensional mass cytometry to extensively phenotype auto- (pre-proinsulin), neo- (insulin-DRIP) and virus- (Cytomegalovirus) reactive CD8 T-cells in peripheral blood mononuclear cells (PBMCs) of T1D patients. A panel of 33 monoclonal antibodies was designed to characterize these cells at the single-cell level. HLA-A2 class I tetramers were used for the detection of antigen-specific CD8 T-cells. | ||||
| Conclusion: | We revealed profound heterogeneity between individuals within the CD8 T-cell compartment. Single-cell analysis of pre-proinsulin, insulin-DRIP and CMV CD8 T-cells showed that the detected specificities were heterogeneous between and within patients. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
