Experiment Overview

Repository ID: FR-FCM-Z586 Experiment name: Venetoclax treatment in CLL patients has limited impact on circulating T and NK cells MIFlowCyt score: 83.75%
Primary researcher: Charis Teh PI/manager: Charis Teh Uploaded by: Charis Teh
Experiment dates: 2017-04-01 - 2022-04-30 Dataset uploaded: Apr 2022 Last updated: May 2022
Keywords: [CLL] [T cells] [venetoclax] Manuscripts:
Organizations: The Walter and Eliza Hall Institute of Medical Research, Immunology, Melbourne, VIC3052 (Australia)
Purpose: o profile circulating blood cells from CLL patients before and after treatment with venetoclax using flow cytometry
Conclusion: Venetoclax has minimal impact on circulating T or NK cells
Comments: None
Funding: This work was supported by: an Australian NHMRC Fellowships 1089072 (C.E.T), 1090236 and 1158024 (D.H.D.G), 1078730 and 1175960 (G.J.L); a Fulbright Australia-America Postdoctoral Fellowship (C.E.T); a Victorian Cancer Agency Mid-Career Fellowship MCRF20026 (C.E.T); Cancer Council of Victoria Grants-in-Aid 1146518 and 1102104 (D.H.D.G); Perpetual Impact Philanthropy Founding (C.E.T.); Australian NHRMC grants 2002618 (C.E.T), 1113577, 1016701, 1079560 (A.W.R/D.C.S.H); 1113133, 1153049 (G.J.L), the Metcalf Family (A.H.W); the Leukemia and Lymphoma Society, US, Specialized Center of Research [SCOR] grant 7015-18 (A.H.W); a Medical Research Future Fund grant 1141460 (A.H.W.); National Breast Cancer Foundation Australia IIRS-19-004; Breast Cancer Research Foundation (BCRF-20-182) (G.J.L), Investigator Initiated Study support from AbbVie and Genentech (Roche) for the mBEP study (ACTRN12615000702516) (G.J.L).
Quality control: - An anchor healthy blood sample is used in each run - All antibodies were titrated to determine ideal concentrations of used - Where samples needed to be ran in two batches on different days, cytometers with the same settings were used.

Experiment variables

· pre-venetoclax CLL_12_01.fcs · CLL_13_02.fcs · CLL_15_02.fcs · CLL_16_01.fcs · CLL_18_01.fcs · CLL_19_01.fcs · CLL_20_02.fcs · CLL_21_01.fcs · CLL_22_02.fcs · CLL_25_01.fcs · CLL_27_01.fcs · CLL_28_01.fcs · CLL_30_01 .fcs · CLL_31_01.fcs · CLL_33_01.fcs · CLL_35.fcs · CLL_36_01.fcs · CLL_37_01.fcs · CLL_39_01.fcs
· post-venetoclax CLL_12_02.fcs · CLL_13_05.fcs · CLL_15_06.fcs · CLL_16_06.fcs · CLL_18_05.fcs · CLL_19_05.fcs · CLL_20_06.fcs · CLL_21_05.fcs · CLL_22_08.fcs · CLL_25_06.fcs · CLL_27_05.fcs · CLL_28_05.fcs · CLL_30_05.fcs · CLL_31_04.fcs · CLL_33_05.fcs · CLL_35_03.fcs · CLL_36_04.fcs · CLL_37_04.fcs · CLL_39_05.fcs

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