The FlowRepository admin email (flowrepository-admin@isac-net.org) has not been working since December 13th 2018 and is still not working. From January 3rd 2019 the website will now link directly to the admin, Justin, through his email of justinmeskas@gmail.com. If you had sent a support ticket or any other means to connect to the admins of FlowRepository between those two dates, those emails did not go through. Please forward any emails to him, or re-create any issues through the website. Sorry for any inconvenience and thank you for your patience and understanding.

I am getting an error message about conflicting channels, how do I resolve it?

In FlowRepository, a panel is a set of FCS files with the same channels. These have to match in number and the same marker has to be used in the same channel across the FCS files of the whole panel. In addition, the channels have to match in annotations. If this is all consistent across all the FCS files in the experiment then FlowRepository assigns the panels automatically and you typically don't need to worry about it.

If the annotations within your FCS data files aren't fully consistent, then FlowRepository will complain about conflicts as it is not certain how FCS files should be organized and/or what the channels are. In order to resolve those conflicts, you start by clicking on the "Panel 1" (or any other Panel) link in the list of your FCS files.

FCS file list

Next, you will see that FlowRepository detected a certain number of panels. You should verify that this is correct before starting to resolve the conflicts. If it is not, then you may add new panels using the Add Panels box in your top left corner.

Adding panels

Next, please make sure that your experiment files are correctly assigned to the panels. You can move FCS files from one panel to another by selecting them and clicking on "move to". For example, as shown below, the two FCS files in Panel 1 have clearly different reagents and therefore, one of them should be moved to a different panel.

Resolve reagent name conflicts

Finally, you may have to resolve conflicts in channel annotations (reagent names). This depends on the description of your channels in the FCS data files (the value of the $PnS keywords). For example (as shown below), your "APC-A" channel in Panel 2 is sometimes annotated as "IL-17" and sometimes as "IL17".

Resolve reagent name conflicts

All you need to do is click on the Resolve Conflict button, and then select either "use IL17" or "use IL-17" or provide a new annotation.
In some cases, the annotation (reagent name) may be missing in some FCS files. This is demonstrated on the same example: the PE-Cy7-A channel is annotated as "PE-Cy7-A" in 8 FCS files, and the value is left blank in the other 28 files. Again, you can resolve this conflict by clicking on the Resolve Conflict button and selecting what the final description of that channel should be (for all FCS files in that Panel).