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Experiment Overview

Repository ID: FR-FCM-Z24P Experiment name: BM53 Tumor and Virus MIFlowCyt score: 81.85%
Primary researcher: daphné laubreton PI/manager: jacqueline marvel Uploaded by: daphné laubreton
Experiment dates: 2017-03-22 - 2017-05-10 Dataset uploaded: Jun 2019 Last updated: Apr 2024
Keywords: [tumor] [CD8 memory] [virus] [count] Manuscripts: [33554899]
Organizations: CIRI (Centre International de Recherche en Infectiologie) INSERM U1111 UMR5308, Lyon, (France)
Purpose: The purpose of this experiment is to follow the differentiation of naive CD8 T cells in memory CD8 T cells in response to virus infection or tumor growth. To do so, naive C57Bl/6J mice were transferred with naive F5 TCR-transgenic T cells and infected with NP68-expressing vaccinia virus (VV-NP68) or injected with NP68-expressing EL4 tumor cells (EL4-NP68). CD8 T cells count and phenotype was followed in the blood over time
Conclusion: Kinetics of differentiation in responses to tumor- or virus-immunization differ
Comments: None
Funding: Not disclosed
Quality control: All mice were homozygous adult 6-8-week-old at the beginning of experiments. They were healthy and housed in our institute's animal facility under pathogen-free conditions. Age- and sex-matched littermates or provider's delivery groups, which were naive of any experimental manipulation, were randomly assigned to experimental groups (n=5) and co-housed at least for one week prior to experimentation.


Experiment variables

Timepoints
· 4 DS_4dpi_EL4_001_014.fcs · DS_4dpi_EL4_002_015.fcs · DS_4dpi_EL4_003_016.fcs · DS_4dpi_EL4_004_017.fcs · DS_4dpi_EL4_005_018.fcs · DS_4dpi_VV_001_010.fcs · DS_4dpi_VV_002_011.fcs · DS_4dpi_VV_003_012.fcs · DS_4dpi_VV_004_013.fcs
· 6 DS_6dpi_EL4_001_031.fcs · DS_6dpi_EL4_002_032.fcs · DS_6dpi_EL4_003_033.fcs · DS_6dpi_EL4_004_034.fcs · DS_6dpi_EL4_005_035.fcs · DS_6dpi_VV_001_027.fcs · DS_6dpi_VV_002_028.fcs · DS_6dpi_VV_003_029.fcs · DS_6dpi_VV_004_030.fcs
· 7 DS_7dpi-EL4_001_039.fcs · DS_7dpi-EL4_002_040.fcs · DS_7dpi_EL4_003_041.fcs · DS_7dpi_EL4_004_042.fcs · DS_7dpi_EL4_005_043.fcs · DS_7dpi_VV_001_036.fcs · DS_7dpi_VV_002_037.fcs · DS_7dpi_VV_003_038.fcs
· 8 DS_8dpi_EL4_001_048.fcs · DS_8dpi_EL4_002_049.fcs · DS_8dpi_EL4_003_050.fcs · DS_8dpi_EL4_004_051.fcs · DS_8dpi_EL4_005_052.fcs · DS_8dpi_VV_001_044.fcs · DS_8dpi_VV_002_045.fcs · DS_8dpi_VV_003_046.fcs · DS_8dpi_VV_004_047.fcs
· 11 DS_11dpi_EL4_001_014.fcs · DS_11dpi_EL4_002_015.fcs · DS_11dpi_EL4_003_016.fcs · DS_11dpi_EL4_004_017.fcs · DS_11dpi_EL4_005_018.fcs · DS_11dpi_VV_001_010.fcs · DS_11dpi_VV_002_011.fcs · DS_11dpi_VV_003_012.fcs · DS_11dpi_VV_004_013.fcs
· 13 DS_13dpi_EL4_001_014.fcs · DS_13dpi_EL4_002_015.fcs · DS_13dpi_EL4_003_016.fcs · DS_13dpi_EL4_004_017.fcs · DS_13dpi_EL4_005_018.fcs · DS_13dpi_VV_001_010.fcs · DS_13dpi_VV_002_011.fcs · DS_13dpi_VV_003_012.fcs · DS_13dpi_VV_004_013.fcs
· 15 DS_15dpi_EL4_001_013.fcs · DS_15dpi_EL4_002_014.fcs · DS_15dpi_EL4_003_015.fcs · DS_15dpi_EL4_004_016.fcs · DS_15dpi_EL4_005_017.fcs · DS_15dpi_VV_001_010.fcs · DS_15dpi_VV_003_011.fcs · DS_15dpi_VV_004_012.fcs
· 21 DS_21dpi_EL4_001_014.fcs · DS_21dpi_EL4_002_015.fcs · DS_21dpi_EL4_003_016.fcs · DS_21dpi_EL4_004_017.fcs · DS_21dpi_EL4_005_018.fcs · DS_21dpi_VV_001_010.fcs · DS_21dpi_VV_002_011.fcs · DS_21dpi_VV_003_012.fcs · DS_21dpi_VV_004_013.fcs
· 28 DS_28dpi_EL4_001_014.fcs · DS_28dpi_EL4_002_015.fcs · DS_28dpi_EL4_003_016.fcs · DS_28dpi_EL4_004_017.fcs · DS_28dpi_EL4_005_018.fcs · DS_28dpi_VV_001_010.fcs · DS_28dpi_VV_002_011.fcs · DS_28dpi_VV_003_012.fcs · DS_28dpi_VV_004_013.fcs
· 42 DS_42dpi_EL4_001_014.fcs · DS_42dpi_EL4_002_015.fcs · DS_42dpi_EL4_003_016.fcs · DS_42dpi_EL4_004_017.fcs · DS_42dpi_EL4_005_018.fcs · DS_42dpi_VV_001_010.fcs · DS_42dpi_VV_002_011.fcs · DS_42dpi_VV_003_012.fcs · DS_42dpi_VV_004_013.fcs

Conditions
· VV DS_11dpi_VV_001_010.fcs · DS_11dpi_VV_002_011.fcs · DS_11dpi_VV_003_012.fcs · DS_11dpi_VV_004_013.fcs · DS_13dpi_VV_001_010.fcs · DS_13dpi_VV_002_011.fcs · DS_13dpi_VV_003_012.fcs · DS_13dpi_VV_004_013.fcs · DS_15dpi_VV_001_010.fcs · DS_15dpi_VV_003_011.fcs · DS_15dpi_VV_004_012.fcs · DS_21dpi_VV_001_010.fcs · DS_21dpi_VV_002_011.fcs · DS_21dpi_VV_003_012.fcs · DS_21dpi_VV_004_013.fcs · DS_28dpi_VV_001_010.fcs · DS_28dpi_VV_002_011.fcs · DS_28dpi_VV_003_012.fcs · DS_28dpi_VV_004_013.fcs · DS_42dpi_VV_001_010.fcs · DS_42dpi_VV_002_011.fcs · DS_42dpi_VV_003_012.fcs · DS_42dpi_VV_004_013.fcs · DS_4dpi_VV_001_010.fcs · DS_4dpi_VV_002_011.fcs · DS_4dpi_VV_003_012.fcs · DS_4dpi_VV_004_013.fcs · DS_6dpi_VV_001_027.fcs · DS_6dpi_VV_002_028.fcs · DS_6dpi_VV_003_029.fcs · DS_6dpi_VV_004_030.fcs · DS_7dpi_VV_001_036.fcs · DS_7dpi_VV_002_037.fcs · DS_7dpi_VV_003_038.fcs · DS_8dpi_VV_001_044.fcs · DS_8dpi_VV_002_045.fcs · DS_8dpi_VV_003_046.fcs · DS_8dpi_VV_004_047.fcs
· EL4 DS_11dpi_EL4_001_014.fcs · DS_11dpi_EL4_002_015.fcs · DS_11dpi_EL4_003_016.fcs · DS_11dpi_EL4_004_017.fcs · DS_11dpi_EL4_005_018.fcs · DS_13dpi_EL4_001_014.fcs · DS_13dpi_EL4_002_015.fcs · DS_13dpi_EL4_003_016.fcs · DS_13dpi_EL4_004_017.fcs · DS_13dpi_EL4_005_018.fcs · DS_15dpi_EL4_001_013.fcs · DS_15dpi_EL4_002_014.fcs · DS_15dpi_EL4_003_015.fcs · DS_15dpi_EL4_004_016.fcs · DS_15dpi_EL4_005_017.fcs · DS_21dpi_EL4_001_014.fcs · DS_21dpi_EL4_002_015.fcs · DS_21dpi_EL4_003_016.fcs · DS_21dpi_EL4_004_017.fcs · DS_21dpi_EL4_005_018.fcs · DS_28dpi_EL4_001_014.fcs · DS_28dpi_EL4_002_015.fcs · DS_28dpi_EL4_003_016.fcs · DS_28dpi_EL4_004_017.fcs · DS_28dpi_EL4_005_018.fcs · DS_42dpi_EL4_001_014.fcs · DS_42dpi_EL4_002_015.fcs · DS_42dpi_EL4_003_016.fcs · DS_42dpi_EL4_004_017.fcs · DS_42dpi_EL4_005_018.fcs · DS_4dpi_EL4_001_014.fcs · DS_4dpi_EL4_002_015.fcs · DS_4dpi_EL4_003_016.fcs · DS_4dpi_EL4_004_017.fcs · DS_4dpi_EL4_005_018.fcs · DS_6dpi_EL4_001_031.fcs · DS_6dpi_EL4_002_032.fcs · DS_6dpi_EL4_003_033.fcs · DS_6dpi_EL4_004_034.fcs · DS_6dpi_EL4_005_035.fcs · DS_7dpi-EL4_001_039.fcs · DS_7dpi-EL4_002_040.fcs · DS_7dpi_EL4_003_041.fcs · DS_7dpi_EL4_004_042.fcs · DS_7dpi_EL4_005_043.fcs · DS_8dpi_EL4_001_048.fcs · DS_8dpi_EL4_002_049.fcs · DS_8dpi_EL4_003_050.fcs · DS_8dpi_EL4_004_051.fcs · DS_8dpi_EL4_005_052.fcs

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