Experiment Overview
| Repository ID: | FR-FCM-Z258 | Experiment name: | BM21 Tumor | MIFlowCyt score: | 85.11% |
| Primary researcher: | daphné laubreton | PI/manager: | jacqueline marvel | Uploaded by: | Christophe Arpin |
| Experiment dates: | 2013-07-12 - 2013-08-28 | Dataset uploaded: | Jul 2019 | Last updated: | Jul 2019 |
| Keywords: | [CD8 T cells] [immune response] [tumor] | Manuscripts: | [28237797] [33554899] | ||
| Organizations: |
CIRI (Centre International de Recherche en Infectiologie) INSERM U1111 UMR5308, Lyon, (France)
|
||||
| Purpose: | The purpose of this experiment is to follow the differentiation of naive CD8 T cells in memory CD8 T cells in response tumor growth. To do so, naive C57Bl/6J mice were transferred with naive F5 TCR-transgenic T cells and injected with NP68-expressing EL4 tumor cells (EL4-NP68). CD8 T cells count and phenotype was followed in the blood over time | ||||
| Conclusion: | Ag-specific CD8 T cell differentiation can be monitored in the blood up to the memory stage. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | . All mice were homozygous adult 6-8-week-old at the beginning of experiments. They were healthy and housed in our institute's animal facility under pathogen-free conditions. Age- and sex-matched littermates or provider's delivery groups, which were naive of any experimental manipulation, were randomly assigned to experimental groups (n=5) and co-housed at least for one week prior to experimentation. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · 5 | J5_SOURIS_001.fcs · J5_SOURIS_002.fcs · J5_SOURIS_003.fcs · J5_SOURIS_004.fcs · J5_SOURIS_005.fcs |
| · 7 | J7_SOURIS_001.fcs · J7_SOURIS_002.fcs · J7_SOURIS_003.fcs · J7_SOURIS_004.fcs · J7_SOURIS_005.fcs |
| · 10 | J10_SOURIS_001.fcs · J10_SOURIS_002.fcs · J10_SOURIS_003.fcs · J10_SOURIS_004.fcs · J10_SOURIS_005.fcs |
| · 14 | J14_SOURIS_001.fcs · J14_SOURIS_002.fcs · J14_SOURIS_003.fcs · J14_SOURIS_004.fcs · J14_SOURIS_005.fcs |
| · 46 | J46_SOURIS_001.fcs · J46_SOURIS_002.fcs · J46_SOURIS_003.fcs · J46_SOURIS_004.fcs · J46_SOURIS_005.fcs |
| Conditions | |
|---|---|
| · EL4 | J10_SOURIS_001.fcs · J10_SOURIS_002.fcs · J10_SOURIS_003.fcs · J10_SOURIS_004.fcs · J10_SOURIS_005.fcs · J14_SOURIS_001.fcs · J14_SOURIS_002.fcs · J14_SOURIS_003.fcs · J14_SOURIS_004.fcs · J14_SOURIS_005.fcs · J46_SOURIS_001.fcs · J46_SOURIS_002.fcs · J46_SOURIS_003.fcs · J46_SOURIS_004.fcs · J46_SOURIS_005.fcs · J5_SOURIS_001.fcs · J5_SOURIS_002.fcs · J5_SOURIS_003.fcs · J5_SOURIS_004.fcs · J5_SOURIS_005.fcs · J7_SOURIS_001.fcs · J7_SOURIS_002.fcs · J7_SOURIS_003.fcs · J7_SOURIS_004.fcs · J7_SOURIS_005.fcs |
