Experiment Overview
| Repository ID: | FR-FCM-Z27L | Experiment name: | IL-33 and IL-5 Family Mediated Activation of Human Eosinophils | MIFlowCyt score: | 63.50% |
| Primary researcher: | Evelyn Angulo | PI/manager: | Sameer Mathur | Uploaded by: | Evelyn Angulo |
| Experiment dates: | 2017-02-01 - 2017-06-20 | Dataset uploaded: | Aug 2019 | Last updated: | Aug 2019 |
| Keywords: | [cytokines] [Eosinophils] [IL-33] | Manuscripts: | |||
| Organizations: |
University of Wisconsin School of Medicine and Public Health, Department of Medicine, Madison, WI (USA)
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| Purpose: | Our goal was to assess IL-33 stimulated cell surface protein expression in comparison to IL-3, IL-5, and eotaxin-1 on human eosinophils. Purified peripheral eosinophils were incubated with these cytokines and then stained for cell surface markers to evaluate expression. | ||||
| Conclusion: | IL-33 has as potent effects on the cell surface marker expression of human eosinophils as IL-3, IL-5 and eotaxin-1. | ||||
| Comments: | None | ||||
| Funding: | Funding was provided by: NIH R21AI122103-01A1, NIH/NIAID T32 AI007635, NIH P01 HL088594 | ||||
| Quality control: | Cytokine stimulations of eosinophils were performed in 7-9 replicates each. Each experiment was run with a unstained unstimulated, and an unstained stimulated controls. | ||||
