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Experiment Overview

Repository ID: FR-FCM-Z28B Experiment name: Lamina propia and MLN cells during H. polygyrus infection MIFlowCyt score: 31.73%
Primary researcher: Laura Ferrer Font PI/manager: Johannes U Mayer Uploaded by: Laura Ferrer Font
Experiment dates: 2019-04-01 - 2019-08-01 Dataset uploaded: Aug 2019 Last updated: Oct 2019
Keywords: [lamina propia] [spectral flow cytometry] [MLN] [helminth] Manuscripts:
Organizations: Malaghan Institute of Medical Research, Malaghan Institute of Medical Research, Wellington, Outside U.S./Canada (New Zealand)
Purpose: Characterisation of intestinal innate and adaptive immune responses for different stages of H. polygyrus infection using our optimised lamina propria cell isolation protocol
Conclusion: We were able to characterise intestinal innate and adaptive immune responses for all stages of H. polygyrus infection using our optimised lamina propria cell isolation protocol, which has previously not been possible.
Comments: Data of 2 gut experiments and 2 mesenteric lymph node experiments used for manual and high-dimensional analysis are provided.
Funding: This work was supported by an ‘In Aid of Research’ grant from the Research For Life foundation awarded to Johannes Mayer and by grants from the Hugh Green Foundation, Maurice Wilkins Centre and the Health Research Council.
Quality control: Viability was assessed in order to make sure that the samples had enough quality to be analysed by flow cytometry. Also, the expression of the different markers was checked in order to make sure that the samples were suitable for analysis.
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