Experiment Overview
Repository ID: | FR-FCM-Z28C | Experiment name: | A single cell atlas of tumor resistance to PD-1 blockade and radiotherapy | MIFlowCyt score: | 22.50% |
Primary researcher: | Amy Wisdom | PI/manager: | Amy Wisdom | Uploaded by: | Amy Wisdom |
Experiment dates: | 2019-08-22 - | Dataset uploaded: | Aug 2019 | Last updated: | Oct 2020 |
Keywords: | None | Manuscripts: | |||
Organizations: |
Duke University Medical Center, Pharmacology and Cancer Biology, Durham, NC (United States)
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Purpose: | Using single-cell RNA sequencing and mass cytometric profiling, we generated a single cell atlas of tumor-infiltrating immune cells from allograft and primary tumors before and after radiation and anti-PD-1 immunotherapy. The mass cytometry (CyTOF) data are uploaded here. | ||||
Conclusion: | None | ||||
Comments: | concatenated files for each group were generated from downsampling CD45+ immune cells only | ||||
Funding: | Not disclosed | ||||
Quality control: | Included 10% EQ Calibration Beads (Fluidigm) |