Experiment Overview
| Repository ID: | FR-FCM-Z2A4 | Experiment name: | A Novel Semiconductor-Based Flow Cytometer with Enhanced Light-Scatter Sensitivity for the Analysis of Biological Nanoparticles | MIFlowCyt score: | 31.50% |
| Primary researcher: | George Brittain | PI/manager: | George Brittain | Uploaded by: | George Brittain |
| Experiment dates: | 2018-08-15 - 2019-03-28 | Dataset uploaded: | Sep 2019 | Last updated: | Mar 2021 |
| Keywords: | [Innate Immune Response] [HIV] [exosomes] [EVs] [Cytoflex] [vaccinia virus strains] [Extracellular Vesicles (EVs)] [Small Particles] [viruses] [VSSC] [MLV] [HSV] [adenovirus] | Manuscripts: | |||
| Organizations: |
Beckman Coulter Life Sciences, Brea, CA (USA)
University of Ottawa, Flow Cytometry & Virometry Core Facility, Ottawa, ON (Canada) |
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| Purpose: | The overall objective of these experiments was to analyze and demonstrate the light-scatter sensitivity of the CytoFLEX flow cytometer for synthetic and biological nanoparticles. A further focus of the article was to describe how various systems and features on the CytoFLEX work in order to help researchers better understand how to optimally utilize the instrument performance, and to clarify the various differences that distinguish it from other conventional flow cytometers. | ||||
| Conclusion: | VSSC sensitivity: EVs >30-60nm. Viruses >81nm. 70nm polystyrene and 98nm silica resolved. | ||||
| Comments: | The instrument and article should help enable small-particle researchers. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Cleaning, optimization, reference standards, extensive training, many repetitions, numerous controls, orthogonal measurements, careful attention to detail, etc. | ||||
