Experiment Overview
| Repository ID: | FR-FCM-Z2C8 | Experiment name: | PRI Tfh-like | MIFlowCyt score: | 53.72% |
| Primary researcher: | Yen Hoang | PI/manager: | Yen Hoang | Uploaded by: | Yen Hoang |
| Experiment dates: | 2016-12-12 - 2020-02-28 | Dataset uploaded: | Nov 2019 | Last updated: | May 2020 |
| Keywords: | [T cell] [SLE] [NZBxW] [Tfh-like] | Manuscripts: | |||
| Organizations: |
University of Wisconsin-Madison, WNPRC, Madison, Wisconsin (USA)
German Rheumatism Research Center, Signal Transduction, Berlin, (Germany) |
||||
| Purpose: | Characterize T cell subpopulations in autoimmune model NZBxW. | ||||
| Conclusion: | PRI uncovered a subpopulation of IL-21+ IFN-ghigh PD-1low CD40Lhigh CXCR5- Bcl-6- T cells specifically expanded in diseased mice. In addition, these cells express high levels of TNF-a and IL-2, and provide B cell help for IgG production in an IL-21 and CD40L dependent manner. This super-functional T cell subset might be a superior driver of autoimmune processes due to a polyfunctional and high cytokine expression combined with Tfh-like properties. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Conditions | |
|---|---|
| · stim | SG170522_Stain1_M1_Stim_001.fcs · SG170522_Stain1_M2_Stim_001_002.fcs · SG170522_Stain2_M1_Stim_007.fcs · SG170522_Stain2_M2_Stim_001_008.fcs · SG170522_Stain3_M1_Stim_001.fcs · SG170522_Stain3_M2_Stim_001_002.fcs · SG170522_Stain4_M1_Stim_012.fcs · SG170522_Stain4_M2_Stim_001_013.fcs · SG20170406_Stain1_stimulated_010.fcs · SG20170406_Stain2_stimulated_014.fcs · Stain1_Mouse1_stim_001.fcs · Stain1_Mouse2_stim_002.fcs · Stain3_Mouse1_stim_005.fcs · Stain3_Mouse2_stim_006.fcs · Stain4_Mouse1_stim_010.fcs · Stain4_Mouse2_stim_011.fcs |
| · FMO | SG180130_S2_4hP+I_FMO1_018.fcs · SG180130_S2_4hP+I_FMO2_019.fcs · SG180130_S3_4hP+I_FMO_022.fcs · SG180413_S2_FMO_4hP+I_005_008.fcs · SG180423_S1_FMO_4hP+I_016.fcs · SG180423_S2_FMO_4hP+I_022.fcs · SG180504_M1_Spleen_4hP+I_FMO_018.fcs · SG180516_M2_Spleen_4hP+I_FMO_006.fcs · SG180914_S2_M1_4hP+I_FMO_005.fcs · SG181012_S1_M1_4hP+I_FMO_003.fcs · SG181012_S2_M2_4hP+I_FMO_007.fcs · SG181012_S3_M1_4hP+I_FMO_011.fcs · SG181026_S1_4hP+I_M1_FMO_003.fcs · SG181026_S2_4hP+I_M1_FMO_003.fcs · SG181026_S3_4hP+I_M2_FMO_003.fcs · SG181026_S4_4hP+I_M2_FMO_003.fcs · SG170501_Stain1_FMO1_5hP+I_017.fcs · SG170501_Stain1_FMO2_5hP+I_001_018.fcs · SG170501_Stain2_FMO1_5hP+I_007.fcs · SG170501_Stain2_FMO2_5hP+I_001_001.fcs · SG170501_Stain3_FMO1_5hP+I_001.fcs · SG170501_Stain3_FMO2_5hP+I_002.fcs · SG170501_Stain4_FMO_5hP+I_007.fcs · SG170522_Stain1_FMO1_005.fcs · SG170522_Stain1_FMO2_006.fcs · SG170522_Stain2_FMO_010.fcs · SG170522_Stain3_FMO1_005.fcs · SG170522_Stain3_FMO2_006.fcs · SG170522_Stain4_FMO_016.fcs · SG170530_Stain2_FMO1_017.fcs · SG170530_Stain2_FMO2_018.fcs · SG170530_Stain3_FMO1_021.fcs · SG170530_Stain3_FMO2_022.fcs · SG170704_FMO1_4hP+I_001_003.fcs · SG170704_FMO2_4hP+I_002_004.fcs · SG170915_S1_4hP+I_FMO1_015.fcs · SG170915_S1_4hP+I_FMO2_016.fcs · SG170915_S2_4hP+I_FMO1_024.fcs · SG170915_S2_4hP+I_FMO2_025.fcs · SG170915_S3_4hP+I_FMO1_019.fcs · SG170915_S4_4hP+I_FMO1_021.fcs · SG170915_S5_4hP+I_FMO1_028.fcs · SG170915_S5_4hP+I_FMO2_029.fcs · SG171220_S1_M3_4hP+I_FMO_005.fcs · SG171220_S2_M3_4hP+I_FMO1_009.fcs · SG171220_S2_M3_4hP+I_FMO2_010.fcs · SG171220_S3_M3_4hP+I_FMO_003.fcs · SG171220_S4_M3_4hP+I_FMO_004.fcs · SG181129_S1_M1_4hP+I_FMO_007.fcs · SG181129_S2_001_M3_4hP+I_FMO_007.fcs · SG181129_S2_M2_4hP+I_FMO_007.fcs · SG181204_S1_M1_4hP+I_FMO_006.fcs · SG181204_S2_M3_4hP+I_FMO_006.fcs · SG20170406_Stain1_FMO1_012.fcs · SG20170406_Stain1_FMO2_013.fcs · SG20170406_Stain2_FMO1_016.fcs · SG20170406_Stain2_FMO2_017.fcs · SG20170627_4hP+I_FMO1_013.fcs · SG20170627_4hP+I_FMO2_014.fcs · Stain1_Mouse1_FMO_004.fcs · Stain3_FMO1_008.fcs · Stain3_FMO2_009.fcs · Stain4_Mouse1_FMO1_013.fcs · Stain4_Mouse1_FMO2_014.fcs |
| · unstim | SG170501_Stain1_Mo1_unstim_014.fcs · SG170501_Stain1_Mo2_unstim_001_015.fcs · SG170501_Stain1_Mo3_unstim_002_016.fcs · SG170501_Stain2_Mo1_unstim_004.fcs · SG170501_Stain2_Mo2_unstim_001_005.fcs · SG170501_Stain2_Mo3_unstim_002_006.fcs · SG170501_Stain3_Mo3_unstim_004.fcs · SG170501_Stain4_Mo1_unstim_004.fcs · SG170501_Stain4_Mo2_unstim_005.fcs · SG170501_Stain4_Mo3_unstim_006.fcs · SG170522_Stain1_M1_unstim_002_003.fcs · SG170522_Stain1_M2_unstim_003_004.fcs · SG170522_Stain2_M1_unstim_002_009.fcs · SG170522_Stain2_M2_unstim_011.fcs · SG170522_Stain3_M1_unstim_002_003.fcs · SG170522_Stain3_M2_unstim_003_004.fcs · SG170522_Stain4_M1_unstim_002_014.fcs · SG170522_Stain4_M2_unstim_003_015.fcs · SG170530_Stain2_unstim_016.fcs · SG170530_Stain3_unstim_020.fcs · SG20170406_Stain1_unstimulated_011.fcs · SG20170406_Stain2_unstimulated_015.fcs · Stain1_Mouse1_unstim_003.fcs · Stain3_Mouse1_unstim_007.fcs · Stain4_Mouse2_unstim_012.fcs |
| · 0hP+Iono | SG180130_S2_0hP+I_017.fcs · SG180130_S3_0hP+I_021.fcs · SG180413_S2_0hP+I_006_009.fcs · SG180423_S1_0hP+I_017.fcs · SG180423_S2_0hP+I_023.fcs · SG180504_M2_Spleen_0hP+I_016.fcs · SG180516_M1_Spleen_0hP+I_005.fcs · SG180613_S2_NZBxW_2_0hP+I_006.fcs · SG180914_S2_M1_0hP+I_004.fcs · SG181012_S1_M1_0hP+I_004.fcs · SG181012_S2_M2_0hP+I_008.fcs · SG181012_S3_M1_0hP+I_012.fcs · SG181012_S3_M2_0hP+I_013.fcs · SG181012_S4_M1_0hP+I_016.fcs · SG181012_S4_M2_0hP+I_017.fcs · SG181026_S1_0hP+I_M1_004.fcs · SG181026_S2_0hP+I_M1_004.fcs · SG181026_S3_0hP+I_M2_004.fcs · SG181026_S4_0hP+I_M2_004.fcs · SG170704_0hP+I_002.fcs · SG170915_S1_0hP+I_014.fcs · SG170915_S2_0hP+I_026.fcs · SG170915_S3_0hP+I_018.fcs · SG170915_S4_0hP+I_022.fcs · SG170915_S5_0hP+I_030.fcs · SG171220_S1_M3_0hP+I_004.fcs · SG171220_S2_M3_0hP+I_011.fcs · SG171220_S3_M3_0hP+I_004.fcs · SG171220_S4_M3_0hP+I_005.fcs · SG181129_S1_M1_0hP+I_004.fcs · SG181129_S1_M2_0hP+I_005.fcs · SG181129_S1_M3_0hP+I_006.fcs · SG181129_S2_001_M1_0hP+I_004.fcs · SG181129_S2_001_M2_0hP+I_005.fcs · SG181129_S2_001_M3_0hP+I_006.fcs · SG181129_S2_M1_0hP+I_004.fcs · SG181129_S2_M2_0hP+I_005.fcs · SG181129_S2_M3_0hP+I_006.fcs · SG181204_S1_M1_0hP+I_004.fcs · SG181204_S1_M3_0hP+I_005.fcs · SG181204_S2_M1_0hP+I_004.fcs · SG181204_S2_M3_0hP+I_005.fcs · SG20170627_0hP+I_012.fcs |
| · 4hP+Iono | SG180130_S2_4hP+I_016.fcs · SG180130_S3_4hP+I_020.fcs · SG180413_S2_M1_old_4hP+I_001.fcs · SG180413_S2_M2_old_4hP+I_001_002.fcs · SG180413_S2_M3_old_4hP+I_003.fcs · SG180413_S2_M4_yg_4hP+I_001_001.fcs · SG180413_S2_M5_yg_4hP+I_002_005.fcs · SG180413_S2_M6_yg_4hP+I_003_006.fcs · SG180423_S1_old1_4hP+I_012.fcs · SG180423_S1_old2_4hP+I_013.fcs · SG180423_S1_yg1_4hP+I_014.fcs · SG180423_S1_yg2_4hP+I_015.fcs · SG180423_S2_old1_4hP+I_018.fcs · SG180423_S2_old2_4hP+I_019.fcs · SG180423_S2_yg1_4hP+I_020.fcs · SG180423_S2_yg2_4hP+I_021.fcs · SG180504_M1_Kidney_4hP+I_020.fcs · SG180504_M1_Liver_4hP+I_015.fcs · SG180504_M1_Lung_4hP+I_013.fcs · SG180504_M1_Spleen_4hP+I_011.fcs · SG180504_M2_Kidney_4hP+I_021.fcs · SG180504_M2_Liver_4hP+I_017.fcs · SG180504_M2_Lung_4hP+I_014.fcs · SG180504_M2_PBMCs_4hP+I_019.fcs · SG180504_M2_Spleen_4hP+I_012.fcs · SG180516_M1_Kidney_4hP+I_010.fcs · SG180516_M1_Liver_4hP+I_003.fcs · SG180516_M1_Lung_4hP+I_008.fcs · SG180516_M1_PBMC_4hP+I_007.fcs · SG180516_M1_Spleen_4hP+I_001.fcs · SG180516_M2_Kidney_4hP+I_011.fcs · SG180516_M2_Liver_4hP+I_004.fcs · SG180516_M2_Lung_4hP+I_009.fcs · SG180516_M2_Spleen_4hP+I_002.fcs · SG180613_S2_NZBxW_1_4hP+I_001.fcs · SG180613_S2_NZBxW_2_4hP+I_002.fcs · SG180914_S2_M1_4hP+I_001.fcs · SG180914_S2_M2_4hP+I_002.fcs · SG180914_S2_M3_4hP+I_003.fcs · SG181012_S1_M1_4hP+I_001.fcs · SG181012_S1_M2_4hP+I_002.fcs · SG181012_S2_M1_4hP+I_005.fcs · SG181012_S2_M2_4hP+I_006.fcs · SG181012_S3_M1_4hP+I_009.fcs · SG181012_S3_M2_4hP+I_010.fcs · SG181012_S4_M1_4hP+I_014.fcs · SG181012_S4_M2_4hP+I_015.fcs · SG181026_S1_4hP+I_M1_001.fcs · SG181026_S1_4hP+I_M2_002.fcs · SG181026_S2_4hP+I_M1_001.fcs · SG181026_S2_4hP+I_M2_002.fcs · SG181026_S3_4hP+I_M1_001.fcs · SG181026_S3_4hP+I_M2_002.fcs · SG181026_S4_4hP+I_M1_001.fcs · SG181026_S4_4hP+I_M2_002.fcs · SG170530_Stain2_4hP+I_015.fcs · SG170530_Stain3_4hP+I_019.fcs · SG170704_4hP+I_001.fcs · SG170915_S1_4hP+I_013.fcs · SG170915_S2_4hP+I_023.fcs · SG170915_S3_4hP+I_017.fcs · SG170915_S4_4hP+I_020.fcs · SG170915_S5_4hP+I_027.fcs · SG171220_S1_M1_4hP+I_001.fcs · SG171220_S1_M2_4hP+I_002.fcs · SG171220_S1_M3_4hP+I_003.fcs · SG171220_S2_M1_4hP+I_006.fcs · SG171220_S2_M2_4hP+I_007.fcs · SG171220_S2_M3_4hP+I_008.fcs · SG171220_S3_M1_4hP+I_001.fcs · SG171220_S3_M3_4hP+I_002.fcs · SG171220_S4_M1_4hP+I_001.fcs · SG171220_S4_M2_4hP+I_002.fcs · SG171220_S4_M3_4hP+I_003.fcs · SG181129_S1_M1_4hP+I_001.fcs · SG181129_S1_M2_4hP+I_002.fcs · SG181129_S1_M3_4hP+I_003.fcs · SG181129_S2_001_M1_4hP+I_001.fcs · SG181129_S2_001_M2_4hP+I_002.fcs · SG181129_S2_001_M3_4hP+I_003.fcs · SG181129_S2_M1_4hP+I_001.fcs · SG181129_S2_M2_4hP+I_002.fcs · SG181129_S2_M3_4hP+I_003.fcs · SG181204_S1_M1_4hP+I_001.fcs · SG181204_S1_M2_4hP+I_002.fcs · SG181204_S1_M3_4hP+I_003.fcs · SG181204_S2_M1_4hP+I_001.fcs · SG181204_S2_M2_4hP+I_002.fcs · SG181204_S2_M3_4hP+I_003.fcs · SG20170627_4hP+I_011.fcs |
| · 5hP+Iono | SG170501_Stain1_Mo1_5hP+I_011.fcs · SG170501_Stain1_Mo2_5hP+I_001_012.fcs · SG170501_Stain1_Mo3_5hP+I_002_013.fcs · SG170501_Stain2_Mo1_5hP+I_001.fcs · SG170501_Stain2_Mo2_5hP+I_001_002.fcs · SG170501_Stain2_Mo3_5hP+I_002_003.fcs · SG170501_Stain3_Mo1_5hP+I_001.fcs · SG170501_Stain3_Mo2_5hP+I_001_002.fcs · SG170501_Stain3_Mo3_5hP+I_002_003.fcs · SG170501_Stain4_Mo1_5hP+I_001.fcs · SG170501_Stain4_Mo2_5hP+I_002.fcs · SG170501_Stain4_Mo3_5hP+I_003.fcs |
| · IonoTitration | SG181204_IonoTitrat_0_006.fcs · SG181204_IonoTitrat_1000_001.fcs · SG181204_IonoTitrat_125_004.fcs · SG181204_IonoTitrat_250_003.fcs · SG181204_IonoTitrat_500_002.fcs · SG181204_IonoTitrat_62_5_005.fcs |
